Sequence requirements for cytochromes P450IIA1 and P450IIA2 catalytic activity: evidence for both specific and non-specific substrate binding interactions through use of chimeric cDNAs and cDNA expression

Cytochrome P450s IIA1 and IIA2, encoded by the CYP2A1 and CYP2A2genes, display 88% amino acid sequence similarities. The dissimilaritiesof sequence between these two enzymes are primarily localizedwithin four discrete regions of the polypeptides that are separatedby regions of absolute sequence identity. IIA1 specificallyhydroxylates the prototype substrate testosterone at the 7 and6 position with a predominance of 7 metabolite. IIA2, on theother hand, hydroxylates this steroid at eight positions onthe molecule, with one of the most abundant metabolites being15hydroxytestosterone. To determine those amino acids responsiblefor the difference in testosterone hydroxylation specificities,chimeras were constructed between IIA1 and IIA2 cDNAs and expressedin cell culture using vaccinia-virus-mediated cDNA expression.Chimeras, in which the first 355 amino acids correspond to asingle enzyme, maintain the specificity associated with thatenzyme. Of six chimeras which have substitutions between aminoacids 161 and 276, two are inactive and the remaining four givesimilar metabolite profiles, in which both 7 and 15 hydroxylationspecificities have been lost. Two of these four chimeras arediametric apposites, suggesting that modification of eitherthe N-terminal or central regions of the enzymes results inconformational changes that prevent the specific binding interactionsresponsible for the narrow regioselectivity associated withIIA1 and 15-hydroxytestosterone formation associated with IIA2.     

An ionene with spirane structure (spiroionene)

The secondary diamine 1,3,5,7-tetrahydro[1,2c:4,5c'] benzodipyrrole (3) and 1,2,4,5-tetrabromomethylbenzene (1) form a polymeric ionene with spirane structure through a repetitive alkylation reaction. The structure of the product could be proven by¹³C-NMR spectroscopy by comparison with suitable reference compounds. Solutions in aqueous methanol exhibit a typical polyelectrolyte effect. Variation of the counterions produces sufficient solubility in organic solvents. From the crystal structure of a similar model compound one can conclude that the synthesized polymer has a rod-like shape.     

Effective electrode configuration for selective stimulation with inner eye prostheses

The quality of visual perception with retinal prostheses strongly depends on the local selectivity. Electrode arrays at the surface of the retina should excite exclusively cells within a local area but they are expected to co-stimulate bypassing axons originating from ganglion cells of the outer regions. Long electrodes parallel to these axons are shown to be good candidates for avoiding the co-stimulation phenomenon. Efficiency of focal excitation depends on the length and resistance of the electrodes.     

Cooperative MAC Design in Multi-hop Wireless Networks: Part I: When Source and Destination are within the Transmission Range of Each Other

Cooperative communication is regarded as a promising technology in future 5G wireless networks to enhance network performance by exploiting time and/or space diversity via distributed terminals. In this paper, we propose a cooperative medium access protocol which addresses three key aspects of cooperative communications from MAC layer perspective, namely, when to cooperate, whom to cooperate with and how to protect ongoing cooperative transmissions. To further improve the protocol performance in dense networks, three techniques are investigated to avoid potential collision among multiple contending relays. Both analysis and simulation results demonstrate that significant improvement in terms of throughput and packet delivery ratio can be achieved by the proposed cooperative protocol.     

Interacting effects of tillage method,nitrogen fertiliser and secondary drainage on winter wheat production on a calcareous clay soil

The response of winter wheat to nitrogen fertiliser within the range 0–200 kg ha^?1 in 40 kg increments applied either in April or in May in two consecutive seasons (1976–77 and 1977–78) was tested in a field experiment on a calcareous clay soil that was either direct-drilled, shallow tine cultivated (5–8 cm), or mouldboard ploughed (23 cm). These cultivation methods had been used on the same plots in the four preceding seasons (1973 to 1976) in a comparison of cultivation systems. A comparison was also made with direct-drilling on land that had been deep tine cultivated (17 cm) during the 1973–76 experiment. In the second season (1977–78) effects were examined of newly drawn mole drains, on land that had been direct-drilled or ploughed. In both seasons the effect of cultivation method on grain yield was small when nitrogen fertiliser was applied at 80–120 kg N ha^?1. Nitrogen top dressings in April gave heavier yields than the equivalent dressings in May, partly because of dry weather after the May applications in both years. There was no interaction between method of cultivation and amount of nitrogen applied in 1976–77, but a significant interaction was detected in 1977–78 which was probably associated with less nitrogen being available in the uncultivated soil during the winter and spring. The results show that the potential yield of direct-drilled crops may have been underestimated in some earlier comparisons of different methods of cultivation where small uniform top dressings of nitrogen were applied to each cultivation treatment. Mole drainage increased yield especially at low rates of nitrogen and after direct drilling, These results indicate that direct-drilled and ploughed land may differ in their drainage requirements.     

Noise in secondary electron emission: the low yield case

Studies concerning assessment of the image quality in scanning electron microscopes and studies evaluating the detective efficiency of the secondary electron (SE) detectors in these microscopes must be based on statistics of SE emission. The vast majority of previous studies have applied Poisson statistics, although their prerequisites have not been satisfied in most cases. This paper is concerned with the limits to the applicability of Poisson statistics to SE emission. Adequate definition of a non-Poisson factor in the variance of the number of SEs emitted is discussed, and a simple formula for this factor is derived for the low yield case in which both the primary and the backscattered electron are assumed not to release more than one SE. These conditions are met with conductive specimens composed of light elements at primary electron (PE) energies of tens of keV. For the lightest specimens, such as carbon, the non-Poisson factor can even be neglected for PEs >10 keV.     

A Scalable System Architecture for High-Throughput Turbo-Decoders

The need for higher data rates is ever rising as wireless communications standards move from the third to the fourth generation. Turbo-Codes are the prevalent channel codes for wireless systems due to their excellent forward error correction capability. So far research has mainly focused on components of high throughput Turbo-Decoders. In this paper we explore the Turbo-Decoder design space anew, both under system design and deep-submicron implementation aspects. Our approach incorporates all levels of design, from I/O behavior down to floorplaning taking deep-submicron effects into account. Its scalability allows to derive optimized architectures tailored to the given throughput and target technology. We present results for 3GPP compliant Turbo-Decoders beyond 100 Mbit/s synthesized on a 0.18 μm standard cell library.     

使用嵌入式MATLAB实现算法与系统级设计整合

引言本篇文章介绍了使用嵌入式MATLAB语言子集实现系统级整合验证的过程。本文通过一个无线多媒体通信系统中增强视频信号接收能力的自适应滤波器算法的例子     

Monodisperse Polymer Capsules: Tailoring Size,Shell Thickness,and Hydrophobic Cargo Loading via Emulsion Templating

The preparation of monodisperse polymer (polydopamine, PDA) capsules by a one‐step interfacial polymerization of dopamine onto dimethyldiethoxysilane (DMDES) emulsion droplets and removal of the DMDES templates with ethanol is reported. The diameters of the PDA capsules can be tailored from 400 nm to 2.4 µm by varying either the DMDES emulsion condensation time or the emulsion concentration used for templating. Further, capsules with defined nanometer‐scale shell thicknesses (ranging from ～10 to 30 nm) can be prepared by adjusting the emulsion concentration. This shell thickness can be increased by repeated interfacial polymerization of dopamine, with three cycles yielding capsules with a shell thickness of up to 140 nm (for a 0.6% v/v suspension). Functional substances, such as organically stabilized magnetic (Fe₃O₄) nanoparticles, quantum dots (CdSe/CdS), and hydrophobic drugs (thiocoraline), can be preloaded in the emulsion droplets, and following PDA coating and DMDES removal, these materials remain encapsulated in the polymer capsules. All of the unloaded and loaded PDA capsules are monodisperse and do not aggregate. This work provides new avenues for the preparation of polymer capsules with defined size and shell thickness and for the encapsulation of a range of hydrophobic substances.