Researchers" and users" perceptions of the relative standing of biomedical papers in different journals

Journal citation impact factors, which are frequently used as a surrogate measure of research quality, do not correlate well with UK researchers" subjective views of the relative importance of journals as media for communicating important biomedical research results. The correlation varies with the sub-field: it is almost zero in nursing research but is moderate in more “scientific” sub-fields such as multiple sclerosis research, characterised by many authors per paper and appreciable foreign co-authorship. If research evaluation is to be based on journal-specific indicators, then these must cover different aspects of the process whereby research impacts on other researchers and on healthcare improvement. This revised version was published online in June 2006 with corrections to the Cover Date.     

Comparing the Canadian and American WAIS-III normative systems in inpatient neuropsychiatry and forensic psychiatry.

Canadian clinicians have used American norms for adult intelligence tests for more than 60 years. However, the availability of the Canadian norms for the WAIS-III prompts clinicians in Canada to make a decision regarding which norms to use in clinical practice. The purpose of this study is to compare the interpretive effects of applying American and Canadian normative systems in a sample of 100 forensic psychiatry and neuropsychiatry inpatients. The Canadian normative system yielded scores that were systematically lower than the American scores. Thus, clinicians may conclude greater impairment in intellectual ability when using Canadian norms. The index scores that were most similar were Processing Speed followed by Verbal Comprehension. With the exception of these two indices, clinically different conclusions might be derived on 13-21% of individual IQ or Index scores for these patients. The scores that differed the most were the Full Scale IQ and the Performance IQ. The subtests, in general, were highly similar across the normative systems, although scores using the Canadian norms were systematically lower. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Awareness of faces is modulated by their emotional meaning.

A central question in perception is how stimuli are selected for access to awareness. This study investigated the impact of emotional meaning on detection of faces using the attention blink paradigm. Experiment 1 showed that fearful faces were detected more frequently than neutral faces, and Experiment 2 revealed preferential detection of fearful faces compared with happy faces. To rule out image artifacts as a cause for these results, Experiment 3 manipulated the emotional meaning of neutral faces through fear conditioning and showed a selective increase in detection of conditioned faces. These results extend previous reports of preferential detection of emotional words or schematic objects and suggest that fear conditioning can modulate detection of formerly neutral stimuli. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Development of GIS-Based Model to Estimate Relative Reductions in Crop Yield due to Salinity and Waterlogging

A model is introduced that utilizes geographic information systems (GIS) to predict relative reductions in crop yield due to salinity and waterlogging at a field-scale by incorporating spatially and temporally variable crop, climatic, and irrigation data to simulate crop yields. This model utilizes soil and water data commonly collected in field-scale studies. The model’s algorithms are integrated into a GIS (ARCVIEW 3.2) as an extension. The result is a model that does not require extraordinary data collection but will provide practical insight into the spatial effects of salinity and waterlogging on crop yields.     

Induction of human breast cancer cell apoptosis from G2/M preceded by stimulation into the cell cycle by Z-1,1-dichloro-2,3-diphenylcyclopropane

We have shown previously that Z-1,1-dichloro-2,3-diphenylcyclopropane (a.k.a. Analog II, A(II)) inhibits human breast cancer cell proliferation regardless of estrogen receptor status or estrogen sensitivity, and that its cellular targets include microtubules. In the present study, we investigated the apoptosis-inducing effects of A(II). MCF-7, MCF-7/LY2, and MDA-MB-231 cells all showed nuclear fragmentation in response to 100 microM A(II) when stained with Hoechst 33342 and examined by fluorescence microscopy. Pulsed field gel electrophoretic analysis showed that each of the cell lines also developed specific high molecular weight DNA fragments: a low level of 1-2 Mb fragments appeared after 6 hr, while 30-50 kb fragments accumulated subsequently. At 24 hr of drug exposure, the majority of cells became nonadherent, and the 30-50 kb fragments were restricted to detached MCF-7 and MDA-MB-231 cells. Both adherent and detached MCF-7/LY2 cells exhibited these fragments. A previous study by single-color (propidium) flow cytometry demonstrated that A(II) blocks MDA-MB-231 cells in G2/M of the cell cycle. More refined analyses in the present study showed this same result for MDA-MB-231 cells, but MCF-7 and MCF-7/LY2 cells did not reveal apparent drug-induced cell cycle block. A(II) demonstrated growth inhibitory, cell cycle-perturbing, and hypodiploidy-inducing activity against other human breast carcinoma lines, i.e. BT-20, CAMA-1, and SKBR-3, but no such actions in the non-tumorigenic, "normal" human breast epithelial line MCF-10A. Bromodeoxyuridine labeling and two-color flow cytometric analysis, however, suggested that A(II) caused stimulation into S phase, and that G2/M was the phase of the cell cycle from which cells apoptosed. A(II) caused cell rounding, detachment from the growth matrix, and nuclear shrinkage and fragmentation in parallel with biochemical changes. Cycloheximide inhibited A(II)-induced cell death, indicating that its toxicity requires de novo protein synthesis.     

In vitro translation of a 2.3-kb splicing variant of the hamster pgp1 gene whose presence in transfectants is associated with decreased drug resistance

Adherent macrophage populations derived from monocytes isolated from peripheral blood were evaluated for their ability to "shed" the membrane-associated receptor for TNF-alpha (TNFR) following exposure to a calcium ionophor (A23187) and a synthetic chemotactic peptide (fMLP) reagent. A soluble fraction of TNFR was detected in "cell-free" supernatant produced by stimulated macrophage populations applying 125I-TNF-alpha and biotinylated TNF-alpha ligand-binding analysis (96-well format) in combination with conventional autoradiographic techniques. Approximate molecular weight of the shed TNFR glycoprotein fraction was estimated to be 75 kDa based on interpretation of nondenaturing PAGE gels transferred laterally onto sheets of nitrocellulose membrane subsequently probed by ligand-binding analysis applying 125I-TNF-alpha and biotinylated TNF-alpha as detection modalities. Immunorecognition techniques were also employed to detect TNFR fragments shed from macrophages using biotinylated anti-TNFR Type II (75 kDa) monoclonal antibody in combination with conjugated strepavidin:HRPO and a chemiluminescent substrate reagent. In an effort to identify the class of enzyme directly mediating TNFR Type II (75 kDa) shedding, a spectrum of carboxyl- (e.g., aspartate), hydroxyl- (e.g., serine), thiol (e.g., cysteine), and metalo- (e.g., Ca2+, Mg2+) protease-inhibiting agents were evaluated. Experimental findings implied that a carboxy (aspartate) peptidase, and possibly to a lesser extent, serine (hydroxyl), and thiol (cysteine) peptidases participate in macrophage TNFR Type II (75 kDa) shedding phenomena. Subsequent investigations demonstrated that the carboxy (aspartate) peptidase cathepsin-D promoted liberation of TNFR Type II (75 kDa) in unactivated populations of adherent macrophages. In an effort to complement these observations, a protein fraction with presumed carboxy (aspartate) protease activity was isolated from the cell-free supernatant generated by activated populations of adherent macrophages using immobilized pepstatin-A beaded agarose. Exposure of unstimulated populations of adherent macrophages to the partially purified pepstatin-A binding protein fractions resulted in the liberation of a soluble TNFR Type II (75 kDa) fragment based on interpretation of ligand-binding and immunorecognition analysis of samples developed by SDS-PAGE/PAGE format and transferred onto sheets of nitrocellulose membrane. The molecular weight of the macrophage pepstatin-A binding protein fraction was estimated to be 47-52 kDa with lesser bands also visible at approximately 26-32 kDa, and 100 kDa based on SDS-PAGE analysis. Nondenaturing hemoglobin-PAGE substrate gel analysis of protein fractions possessing pepstatin-A binding-avidity detected a protease with a molecular weight of approximately 47-52 kDa that proteolytically digested hemoglobin, in addition to a synthetic cathepsin-D specific peptide substrate. Collective interpretation of these experimental findings directly corresponds with many of the physical (molecular) and functional (biochemical) characteristics known to be associated with the leukocyte carboxy (aspartate) peptidase cathepsin-D, which is a non-metaloprotease known to exert relatively limited proteolytic activity.     

Frequency response subtraction for simple measurement of intrinsiclaser dynamic properties

The authors describe a new technique for extracting the intrinsic laser-diode dynamic properties accurately. This simple technique eliminates the need for accurate microwave calibration of the test equipment and problems of microwave reflections, nonideal frequency response of laser mount, and detector. The effect of the parasitic components of the laser diode are also eliminated from the results so that measurements of important dynamic properties of the laser can be found up to high frequencies (10-20 GHz) on standard laser diodes. The techinque being used to measure variations of resonance peak and damping factor at different bias levels for a standard bulk active region 1.3 μm laser diode is shown     

16 Gbit/s all-optical demultiplexing using four-wave mixing

A novel, purely optical technique for demultiplexing high-speed time-division multiplexed data is proposed and demonstrated. The technique uses optical fibre nonlinearity induced four-wave mixing with a data signal and a probe signal located at different wavelengths. Using only semiconductor laser light sources, 1:4 demultiplexing of 20 ps long, subpicojoule, 2/sup 15/-1 RZ pulses at a data rate of 16 Gbit/s with less than 1 dB penalty is demonstrated.<>