Comparison of the gel-forming ability and gel properties of α-lactalbumin,lysozyme and myoglobin in the presence of β-lactoglobulin under high pressure

α-Lactalbumin (α-La) and lysozyme (LZM) each contain four disulfide bonds but no free SH group, whereas myoglobin (Mb) possesses no disulfide bond or free SH group. In this work, the pressure-induced gelation of α-La, LZM and Mb in the absence and in the presence of β-lactoglobulin (β-Lg) was studied. Solutions of α-La, LZM and Mb (1–24%, w/v) did not form a gel when subjected to a pressure of 800 MPa and circular dichroism analysis revealed that both α-La and LZM are pressure-resistant proteins. In the presence of β-Lg (5%, w/v), however, a pressure-induced gel formed for α-La and LZM (each 15%, w/v) but not for Mb (15%, w/v). One- and two-dimensional SDS-PAGE demonstrated the disulfide cross-linking of proteins was responsible for the gelation. Although α-La and LZM are homologous and have the same disulfide bond arrangement, the texture and appearance of the gels formed from α-La/β-Lg and LZM/β-Lg were markedly different even when induced under the same experimental conditions. Microscopic analysis indicated that phase separation occurs during the gelation of LZM/β-Lg but not during the gelation of α-La/β-Lg. NMR relaxation measurement revealed that the association of water molecules with the protein matrix in the α-La/β-Lg gel is tighter compared to that in the LZM/β-Lg gel. These results indicate that the gel-forming ability of a globular protein under high pressure is related to the primary structure of the protein, and that the gel properties depend on the cross-linking reaction and on the phase behavior of protein dispersion under high pressure.     

Feature extraction of protein expression levels based on classification of functional foods with SOM

We investigated the relations between the physiological activities and protein expression levels of functional foods using a self-organizing map (SOM). The input vectors to the SOM were the protein expression levels and the physiological activity. A competitive node has two kinds of weights: one is for protein expression levels, and the other is for physiological activity. A winner node is decided by the distance between the values of protein expression levels described in the input vector and the corresponding weights only. Then all weights, including those for physiological activity in each node, are updated. Therefore each node has an artificially generated value of physiological activity. Finally, the nodes can be categorized by the abovementioned physiological activity. A well-trained SOM gives us information about the relations between physiological activities and protein expression levels. This work was presented in part at the 13th International Symposium on Artificial Life and Robotics, Oita, Japan, January 31–February 2, 2008     

Improved drift-flux correlation to enhance the prediction of void fraction in nuclear reactor fuel bundles at low flow and elevated pressure conditions

ABSTRACT

The EPRI (1991) and the Clark et al. (2014) drift-flux correlations implemented in RELAP5/MOD3 code were benchmarked against the low-pressure void fraction data collected by Clark et al. (2014) at Purdue University and the high-pressure data collected by Anklam and Miller (1982) at ORNL. The Clark et al. correlation (2014) was found to perform best at low pressures of Clark et al. tests (2014), but the accuracy was deteriorated at higher pressures of Anklam and Miller tests (1982). To account for this effect, a pressure scaling factor has been added to the distribution parameter correlation to reduce the distribution parameter peaking at high pressures. This factor is dependent on the density ratio and it eliminates the distribution parameter enhancement effect for pressures over 0.5 MPa. It was confirmed that the new correlation with the correction improved the void fraction prediction performance at a higher pressure of Anklam and Miller tests (1982). The new correlation provides a transition between the Clark et al. correlation (2014) at low pressures and the correlation of Ozaki et al. (2013) and Ozaki and Hibiki (2015) at high pressures, which is excellent both in physical characteristics and scalability of two-phase flow in rod bundles.     

Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG/OCIF inhibits osteoclastogenesis in vitro

The morphogenesis and remodeling of bone depends on the integrated activity of osteoblasts that form bone and osteoclasts that resorb bone. We previously reported the isolation of a new cytokine termed osteoclastogenesis inhibitory factor, OCIF, which specifically inhibits osteoclast development. Here we report the cloning of a complementary DNA of human OCIF. OCIF is identical to osteoprotegerin (OPG), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. Recombinant human OPG/OCIF specifically acts on bone tissues and increases bone mineral density and bone volume associated with a decrease of active osteoclast number in normal rats. Osteoblasts or bone marrow-derived stromal cells support osteoclastogenesis through cell-to-cell interactions. A single class of high affinity binding sites for OPG/OCIF appears on a mouse stromal cell line, ST2, in response to 1,25-dihydroxyvitamin D3. An anti-OPG/OCIF antibody that blocks the binding abolishes the biological activity of OPG/OCIF. When the sites are blocked with OPG/OCIF, ST2 cells fail to support osteoclastogenesis. These results suggest that the sites are involved in cell-to-cell signaling between stromal cells and osteoclast progenitors and that OPG/OCIF inhibits osteoclastogenesis by interrupting the signaling through the sites.     

日本川崎钢铁公司10年间轧钢技术研究的进展

川崎钢铁公司10年间在轧制理论、弹-塑性理论、摩擦学、有限元法(F刚)等数值解析的各种技术的基础上,以大幅提高设备生产率和提高高附加值产品的质量为目的,进行了以热带钢无头轧制技术为代表的各种开发.介绍了这些研究中几个主要的具体实例:(D热轧薄板技术:自由轧制、热轧摩擦技术和千叶钢铁所第三热带钢轧机中的新技术;(2)冷轧薄板技术:K-WRS轧机的开发和发展,提高不锈钢光洁度的技术;(3)H型钢轧制技术;(4)解析技术.     

Hot water resistance of glass‐fiber and glass‐bead reinforced thermoplastics

The hot water resistance of three kinds of short glass fiber or glass bead‐reinforced plastics [polyphenyleneether (PPE), polyphenylenesulfide (PPS), and polyoxymethylene (POM)] was studied by hot water immersion testing, tensile testing and water‐hammer fatigue testing. It was found that the degradation of the strength was observed only for the reinforced plastics under hot water immersion and that the change of the tensile strength was most drastic in glass fiber‐reinforced PPS (GFPPS). Scanning electron microscope (SEM) observations of the tensile fracture surface revealed that the change in tensile strength was attributable to the deterioration of the interface between the glass fiber and the matrix resin. The results of acoustic emission analysis also supported the conclusion that the change in strength was due to the deterioration of the interface. Although the change in the tensile strength of glass fiber‐reinforced PPE (GFPPE) was small compared with that of GFPPS, debonding between the glass fiber and the matrix resin and surface cracks was observed on the surface of the GFPPE specimens.