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91.
Characterization of Crude and Partially Purified Lipase from Geotrichum candidum Obtained with Different Nitrogen Sources 下载免费PDF全文
Rafael Resende Maldonado Eduardo Luiz Pozza Elizama Aguiar-Oliveira Fátima Aparecida Almeida Costa Francisco Maugeri Filho Maria Isabel Rodrigues 《Journal of the American Oil Chemists' Society》2016,93(10):1355-1364
Lipases from Geotrichum candidum were produced in two different medium: A = 12 % (w/v) clarified corn steep liquor (CCSL) + 0.6 % (w/v) soybean oil (SO) and B = 3.5 % (w/v) yeast hydrolysate (YH) + 0.7 % (w/v) SO. Lipases were partially purified from both media by hydrophobic interaction chromatography using 3.0 mol L?1 of NaCl as mobile phase, and they were characterized in the crude and partially purified forms. The recovery of lipase activity from CCSL and YH via HIC were 96 and 94.3 %, and the purification factors were 44.3 and 86.7‐fold, respectively. All evaluated lipases had similar optimum pH (7.0–7.7), but, for the CCSL crude lipase, optimum temperature (47 °C) was 10 °C higher than others lipases evaluated. CCSL crude lipase possessed a higher thermo stability than YH crude lipase, e.g., at 37 °C (pH 7.0) the half‐life of CCSL crude lipase was 19.25 h and at pH 8.0 (30 °C) the half‐life was 48 h, which are five and ten times higher than with YH crude lipase, respectively. On the other hand, the YH crude lipase possessed a higher catalytic constant (kcat = 2.3 min?1) but with almost the same catalytic efficiency (Km/kcat = 32.12 mg mL min?1) in relation to CCSL crude lipase. The lipases differ in biocatalytic properties between substrates, suggesting that the two lipases can be employed for different applications. 相似文献
92.
Marta Louano Joana Oliveira Isabel Martins Rui Vaz Isaura Tavares 《International journal of molecular sciences》2022,23(7)
Pain transmission at the spinal cord is modulated by noradrenaline (NA)-mediated actions that arise from supraspinal areas. We studied the locus coeruleus (LC) to evaluate the expression of the cathecolamine-synthetizing enzyme tyrosine hydroxylase (TH) and search for local oxidative stress and possible consequences in descending pain modulation in a model of hydrocephalus, a disease characterized by enlargement of the cerebral ventricular system usually due to the obstruction of cerebrospinal fluid flow. Four weeks after kaolin injection into the cisterna magna, immunodetection of the catecholamine-synthetizing enzymes TH and dopamine-β-hydroxylase (DBH) was performed in the LC and spinal cord. Colocalization of the oxidative stress marker 8-OHdG (8-hydroxyguanosine; 8-OHdG), with TH in the LC was performed. Formalin was injected in the hindpaw both for behavioral nociceptive evaluation and the immunodetection of Fos expression in the spinal cord. Hydrocephalic rats presented with a higher expression of TH at the LC, of TH and DBH at the spinal dorsal horn along with decreased nociceptive behavioral responses in the second (inflammatory) phase of the formalin test, and formalin-evoked Fos expression at the spinal dorsal horn. The expression of 8-OHdG was increased in the LC neurons, with higher co-localization in TH-immunoreactive neurons. Collectively, the results indicate increased noradrenergic expression at the LC during hydrocephalus. The strong oxidative stress damage at the LC neurons may lead to local neuroprotective-mediated increases in NA levels. The increased expression of catecholamine-synthetizing enzymes along with the decreased nociception-induced neuronal activation of dorsal horn neurons and behavioral pain signs may indicate that hydrocephalus is associated with alterations in descending pain modulation. 相似文献
93.
Anna-Katharina Hofer Julia Rabitsch Dagmara Jutrzenka-Trzebiatowska Christoph Hofstetter Isabel Gavalda-Velasco Josef Schlacher Martin Schwentenwein Raul Bermejo 《International Journal of Applied Ceramic Technology》2022,19(1):174-180
Fabrication of 3D-printed ceramic parts with high complexity and high spatial resolution often demands low wall thickness as well as high stiffness at the green state, whereas printing simpler geometries may tolerate thicker, more compliant walls with the advantage of a rapid binder-burn-out and sintering process. In this work, the influence of the binder system on the thermophysical properties of 3D-printed stabilized zirconia ceramics was investigated. Samples were fabricated with the lithography-based ceramic manufacturing (LCM) technology using two different photosensitive ceramic suspensions (LithaCon 3Y230 and LithaCon 3Y210), with the same ZrO2 powder. A significant difference in stiffness in the green state (~3 MPa vs. ~32 MPa for LithaCon 3Y230 and LithaCon 3Y210, respectively) was measured, associated with a rather loose or a linked network formed in the binder due to photopolymerization. Both materials reached high relative densities, that is, >99%, exhibiting a homogeneous fine-grained microstructure. No significant differences on the coefficient of thermal expansion (11.18 ppm/K vs. 11.17 ppm/K) or Young's modulus (207 GPa vs. 205 GPa) were measured, thus demonstrating the potential of tailoring binder systems to achieve the required accuracy in 3D-printed parts, without detrimental effects on material's microstructure and thermophysical properties at the sintered state. 相似文献
94.
Melis ArinPetra Lommens Nursen AvciSimon C. Hopkins Klaartje De BuysserIoannis M. Arabatzis Ioanna FasakiDirk Poelman Isabel Van Driessche 《Journal of the European Ceramic Society》2011,31(6):1067-1074
In this work, aqueous chemical solution deposition route suited for inkjet printing is used for the synthesis of photocatalytically active TiO2 coatings. Environmentally friendly precursor solutions with electromagnetic ink-jet printing, allows cheap and simple processing of TiO2 films on glass. The hydrolysis reaction of water sensitive titanium alkoxide (Ti-alkoxide) precursor is controlled by adding complexing agents as citric acid and triethanolamine prior to water addition, and aqueous stable solutions are achieved. The pH of the solutions is brought to neutral to guarantee flexible processing, avoid damage to substrates and equipment. Solution parameters are adapted to obtain optimal gelation conditions and good jettability. The influence of processing parameters on the phase formation and surface morphology is studied by thermogravimetric analysis and differential thermal analysis (TGA/DTA), X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The photocatalytic activity of the films is evaluated by the degradation of methyl orange. 相似文献
95.
96.
The bulk modification of SBS rubber with maleic anhydride in a mixing chamber of a Haake rheomixer was studied. The effect of temperature, maleic anhydride, and benzoyl peroxide concentrations on the grafting efficiency was evaluated. High grafting efficiency was achieved when the ratio of peroxide and maleic anhydride concentration was high. On the other hand, on this condition high insoluble fraction was generated. The addition of a diamine, 4,4′‐diaminediphenylmethane to the reaction mixture minimizes the amount of insoluble polymer. However, the grafted MAH content also decreases. The graft copolymer was characterized by infrared spectroscopy and the grafting extension was determined by titration. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 2953–2960, 2002; DOI 10.1002/app.10355 相似文献
97.
This article concerns the in situ compatibilization of immiscible isotactic polypropylene/butadiene‐styrene‐butadiene triblock copolymer blends (i‐PP/SBS) by means of a reactive mixture. For this purpose, maleated PP (PP‐MAH) and SBS (SBS‐MAH) were used as functionalized polymers and 4,4′‐diaminediphenylmethane was used as a coupling agent between maleated polymers, resulting in a graft copolymer. Binary blends i‐PP/SBS, nonreactive ternary blends i‐PP/PP‐MAH/SBS, and reactive ternary blends i‐PP/PP‐MAH/SBS‐MAH with varying diamine and anhydride molar ratios were prepared. Torque measurements suggest a graft copolymerization during the melt blending for ternary reactive blends, but the extension of the grafting does not vary with the diamine and anhydride molar ratio, but with the elastomer concentration. The morphology of the blends was investigated by scanning electron microscopy. The morphology of binary and ternary nonreactive blends is similar, exhibiting elastomer domains disperse in the i‐PP matrix, whose sizes increase with elastomer concentration. On the other hand, the elastomer domain size in the ternary reactive blends is practically independent of the blends composition and of the diamine and anhydride molar ratio. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 85: 847–855, 2002 相似文献
98.
Isabel Mínguez-Mosquera Lourdes Gallardo-Guerrero María Roca 《Journal of the American Oil Chemists' Society》2002,79(1):93-99
Virgin olive oil is a highly valued product, and it is important to optimize extraction yield. The pectic composition and
the related enzymatic activities, present in the raw material, are variables that may affect that process. The pectinolytic
activities producing modifications in the pectic matter of olive fruits (variety Hojiblanca) during ripening and the associated
changes in texture were studied. Pectinesterase (PE) activity increased with ripeness until reaching a peak when anthocyanin
synthesis in the fruit became marked (turning color stage). From then on, it decreased. In contrast, polygalacturonase (PG)
activity in the ripe-green fruit decreased sharply when anthocyanin formation began (small reddish spots stage) and then increased,
reaching a maximum in the ripe-black fruit. Parallel changes were noted in the texture and pectic content of the olives, related
to endo- and exo-PG enzymatic activities, together with a decrease in the degree of esterification of the pectic matter, which
could be associated with PE action. The distribution of the pectic fractions in the raw material and the changes in them during
the olive oil extraction process showed the role of PE and PG in the fruits that was related to the yield of extracted oil. 相似文献
99.
Lourdes?Gallardo-Guerrero María?Roca M?Isabel Mínguez-MosqueraEmail author 《Journal of the American Oil Chemists' Society》2002,79(1):105-109
The present work has studied the content and type of pigments present in olive fruits and the respective oils and alperujos.
The concentration of isochromic pigment fractions—chlorophylls and carotenoids—decreased with fruit ripening, more markedly
in the former than in the latter. This implied that the ratio between the two pigment fractions also decreased in parallel
in the three products studied. However, the value in the oil was lower than in the fruit, and that in the alperujo was much
higher. During the extraction process, the release of acids may have caused pheophytinization reactions in the chlorophyll
fraction, increasing pheophytin content in alperujos and oils, whereas the carotenoid fraction was affected only in the alperujo.
Chlorophyll b derivatives were destroyed in greater proportion than chlorophyll a derivatives during transfer to the oil. During processing the destruction of lutein was greater than that of β-carotene.
The balance of matter between fruit, alperujo, and oil indicated that not all the fruit pigmentation was released from the
structures, and most remained occluded in the alperujo. The rest of the pigmentation, and particularly the chlorophyll fraction,
was partly destroyed during its transfer to the oil. 相似文献
100.
Dr. Sofia Gama Dr. Filipa Mendes Dr. Teresa Esteves Dr. Fernanda Marques Dr. António Matos Dr. José Rino Dr. Joana Coimbra Dr. Mauro Ravera Dr. Elisabetta Gabano Dr. Isabel Santos Dr. António Paulo 《Chembiochem : a European journal of chemical biology》2012,13(16):2352-2362
New [PtCl(pz*NN)]n+ complexes anchored by pyrazolyl‐diamine (pz*NN) ligands incorporating anthracenyl or acridine orange DNA‐binding groups have been synthesized so as to obtain compounds that would display synergistic effects between platination and intercalation of DNA. Study of their interaction with supercoiled DNA indicated that the anthracenyl‐containing complex L2Pt displays a covalent type of binding, whereas the acridine orange counterpart L3Pt shows a combination of intercalative and covalent binding modes with a strong contribution from the former. L2Pt showed a very strong cytotoxic effect on ovarian carcinoma cell lines A2780 and A2780cisR, which are, respectively, sensitive to and resistant to cisplatin. In these cell lines, L2Pt is nine to 27 times more cytotoxic than cisplatin. In the sensitive cell line, L3Pt showed a cytotoxic activity similar to that of cisplatin, but like L2Pt was able significantly to overcome cisplatin cross‐resistance. Cell‐uptake studies showed that L2Pt accumulates preferentially in the cytoplasm, whereas L3Pt reaches the cell nucleus more easily, as clearly visualized by time‐lapse confocal imaging of live A2870 cells. Altogether, these findings seem to indicate that interaction with biological targets other than DNA might be involved in the mechanism of action of L2Pt because this compound, despite having a weaker ability to target the cell nucleus than L3Pt , as well as an inferior DNA affinity, is nevertheless more cytotoxic. Furthermore, ultrastructural studies of A2870 cells exposed to L2Pt and L3Pt revealed that these complexes induce different alterations in cell morphology, thus indicating the involvement of different modes of action in cell death. 相似文献