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991.
The three-dimensional solution structure of des-[Phe(B25)] human insulin has been determined by nuclear magnetic resonance spectroscopy and restrained molecular dynamics calculations. Thirty-five structures were calculated by distance geometry from 581 nuclear Overhauser enhancement-derived distance constraints, ten phi torsional angle restraints, the restraints from 16 helical hydrogen bonds, and three disulfide bridges. The distance geometry structures were optimized using simulated annealing and restrained energy minimization. The average root-mean-square (r.m.s.) deviation for the best 20 refined structures is 1.07 angstroms for the backbone and 1.92 angstroms for all atoms if the less well-defined N and C-terminal residues are excluded. The helical regions are more well defined, with r.m.s. deviations of 0.64 angstroms for the backbone and 1.51 angstroms for all atoms. It is found that the des-[Phe(B25)] insulin is a monomer under the applied conditions (4.6 to 4.7 mM, pH 3.0, 310 K), that the overall secondary and tertiary structures of the monomers in the 2Zn crystal hexamer of native insulin are preserved, and that the conformation-averaged NMR solution structure is close to the structure of molecule 1 in the hexamer. The structure reveals that the lost ability of des-[Phe(B25)] insulin to self-associate is caused by a conformational change of the C-terminal region of the B-chain, which results in an intra-molecular hydrophobic interaction between Pro(B28) and the hydrophobic region Leu(B11)-Leu(B15) of the B-chain alpha-helix. This interaction interferes with the inter-molecular hydrophobic interactions responsible for the dimerization of native insulin, depriving the mutant of the ability to dimerize. Further, the structure displays a series of features that may explain the high potency of the mutant on the basis of the current model for the insulin-receptor interaction. These features are: a change in conformation of the C-terminal region of the B-chain, the absence of strong hydrogen bonds between this region and the rest of the molecule, and a relatively easy accessibility to the Val(A3) residue. 相似文献
992.
S. Bismo P. Duverneuil L. Pibouleau S. Domenech J. -P. Couderc 《Materials and Manufacturing Processes》1995,10(2):241-266
This paper presents the results of our work on a new type of CVD reactor, annular reactor. This equipment is able to deposit pure silicon and also in-situ phosphorus doped silicon on a large number of substrates 相似文献
993.
This paper presents an artificial intelligence approach of using evolutionary programming to estimate the transient and subtransient parameters of a generator under normal operation. The estimation using evolutionary programming is compared with that using a corrected extended Kalman filter. The comparisons with both simulation and micromachine test results show that evolutionary programming is robust to search the real values of parameters even when the data are highly contaminated by noise, while with the extended Kalman filter, the estimation tends to diverge with such data 相似文献
994.
This paper provides a preliminary examination of present and projected land use in Africa to estimate the potential availability of land in 2025 for use in producing biomass energy. Fifty countries are included in the analysis. Future cropland requirements are projected on the basis of average African cereal crop yield improvements since 1972, and minimum nutritional requirements are assumed to be met in 2025 without increasing imports above present absolute levels. Cropland, natural forests and other wilderness areas are excluded from consideration for biomass energy use. Woody biomass energy yields are estimated on the basis of nationally averaged precipitation, using a yield-precipitation correlation for commercial eucalyptus plantations in Brazil. The total African bioenergy production potential in 2025 is estimated to be about 18 EJ per year for a set of baseline assumptions that includes planting only 10% of the available non-crop, non-forest, non-wilderness area with biomass energy crops. A preliminary cost assessment suggests that much of this biomass could be produced for $1–2 GJ−1. A number of uncertainties in the modelling assumptions are examined through a sensitivity analysis. Despite limitations in the model used here, one robust conclusion is that Africa as a whole has a significant biophysical potential for producing biomass energy. This result suggests that more detailed country and sub-country level assessments would be worthwhile to understand better the practical prospects for future biomass energy production in Africa. 相似文献
995.
996.
The available models of saturated double-cage and deep-bar induction machines are the current state-space model and the flux state-space model, where state-space variables are selected either as stator current and currents of both rotor cages, or stator flux linkage and flux linkages of both rotor cages. This paper presents a number of models of saturated double-cage (deep-bar) induction machines where alternative sets of state-spate variables are selected. The method of main flux saturation modelling relies on recently introduced concept of `generalised flux space vector', which has originally been developed for modelling of saturated single-cage induction machines. The procedure and the novel models are verified by experimental study and simulation of self-excitation process in a double-cage induction generator 相似文献
997.
Piquemal F.P.M. Trapon G.R. Geneves G.P.J. 《IEEE transactions on instrumentation and measurement》1996,45(6):918-922
AC measurements of the longitudinal resistance, Rxx, of a quantum Hall effect (QHE) sample have been made in a frequency range from 10 Hz to 10 kHz. The results show no frequency effect on the minimum value of Rxx corresponding to the quantum numbers i=2 and i=4, within the measurement resolution of 0.5 mΩ. Therefore, the influence of frequency on the value of the quantized Hall resistance, RH, should not exceed a few parts in 109 . Some unwanted effects detected during the development of the resistance bridge have been pointed out 相似文献
998.
Laser-induced chemical vapour deposition of silicon films on SiO2/Si (1 0 0) and Si (1 0 0) substrates was studied using ArF laser irradiation of silane/argon gas mixture in parallel to the substrate. The optimal deposition conditions were specified by examination of film morphology at a wide range of irradiation and process parameters. At optimal conditions, specular films were obtained with no powder formation. The effect of deposition parameters, such as laser energy and repetition rate, on the deposition rate and the related film quality, was investigated. 相似文献
999.
High-stability current control in the 10 A range 总被引:1,自引:0,他引:1
This paper reports on the design and evaluation of a high-stability current control developed for energizing the C-field magnet of the high-field cesium standard experiment, currently in progress at the Politecnico di Torino. This source must supply about 0.8 kW dc power with a current stability in the 10-7 range for more than 1 h 相似文献
1000.
A melt-extraction technique, using a sharpened molybdenum wheel, has been used to produce fine oxide ceramic fibres. Wetting of the molybdenum wheel by molten ceramic is a key parameter in the melt-extraction process. Two types of fibre are generally obtained, depending on the extraction speed. At very low wheel speed, fine and uniform fibres of high quality are produced. However, when the wheel speed exceeds a critical velocity, Rayleigh waves are formed on the free surface of the fibres. Moreover, the average fibre thickness first increases with the wheel velocity, then passes through a maximum, decreasing at high velocity. This thickness variation is discussed in terms of both surface tension and viscosity of the liquid ceramics. 相似文献