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11.
T. Isa T. Fukase M. Sasaki M. Koyano N. Taniguchi T. Kimura Y. Isobe H. Negishi 《Journal of Low Temperature Physics》2002,127(1-2):63-79
We have measured electrical resistivity, Hall coefficient, thermoelectric power, and magnetization for charge-density wave (CDW) material 1T-TaS2 single crystals grown by varying the excess sulfur content x
es. We have revealed that a small mid-gap state is formed inside the Mott gap and that anomalous low temperature transport is not governed by the Mott gap state itself but by the mid-gap state. The electric properties of the mid-gap state are modified by increasing x
es (or hole doping), and we have found the insulator-metal transition occurs by hole doping below 60K. 相似文献
12.
Combination of biomolecular interaction analysis and mass spectrometric amino acid sequencing 总被引:6,自引:0,他引:6
Natsume T Nakayama H Jansson O Isobe T Takio K Mikoshiba K 《Analytical chemistry》2000,72(17):4193-4198
We describe an approach for the combination of biomolecular interaction analysis (BIA) and electrospray tandem mass spectrometry (ESI/MS/MS) to obtain sequence information on the affinity-bound proteins on the sensor chip of BIA. The procedure is illustrated with stable and unstable interactions of recombinant proteins, i.e., histidine-tagged protein-Ni2+/NTA and 1,4,5-inositol trisphosphate receptor-ligand interactions. The E. coli lysates expressing the recombinant proteins were passed through the sensor chips, and biomolecular interactions were monitored in real time. The molecules detected on the sensor chip were digested by delivering proteolytic enzyme to the sensing flow cells. The resulting on-chip digested peptide mixture at the mid- to low-femtomole level was recovered on a microcapillary reversed-phase precolumn by an on-line system and analyzed using HPLC-MS/MS. In both cases, unambiguous sequence information on the recombinant proteins isolated on the sensor chip was obtained from only a single run of analysis. The combined BIA-MS/MS may prove to be a general and versatile system to discover novel biomolecular interactions and to analyze protein complexes. 相似文献
13.
Yoshino T Sotome I Ohtani T Isobe S Oshita S Maekawa T 《Journal of electron microscopy》2000,49(3):483-486
Barley cells cut from a sprout were exposed to either air or high-pressure xenon gas for 3 days and the surface of those cells was observed by atomic force microscopy (AFM) to examine the effect of the gas treatment. This method enabled the direct observation of the fresh surface of the barley cells in solution at high resolution. The cuticle layer was preserved on the primary cell wall of 0.48 MPa xenon gas-treated barley cells, while air-treated barley cells lost the cuticle layer from the primary cell wall. These findings indicate that the high-pressure xenon gas treatment is effective to preserve the cuticle layer attached to the primary cell wall. AFM is a powerful tool for the observation of the surface structure of living plant cells in solution. 相似文献
14.
J Suzuki M Isobe S Yamazaki S Horie Y Okubo M Sekiguchi 《Canadian Metallurgical Quarterly》1998,40(2):307-313
BACKGROUND & AIMS: Interleukin 12 (IL-12) is a heterodimeric, macrophage-derived cytokine that is elevated in Crohn's disease (CD). Epstein-Barr virus-induced gene 3 (EBI3) is a recently characterized human glycoprotein that is homologous to the 40-kilodalton chain of IL-12 and forms a heterodimer with the 35-kilodalton chain of IL-12. We investigated the expression of EBI3 in colonic mucosa of normal control subjects, patients with ulcerative colitis (UC), and patients with CD. METHODS: Colonic tissue was analyzed for messenger RNA (mRNA) expression by quantitative polymerase chain reaction and for protein expression by immunohistology and Western blotting. RESULTS: EBI3 mRNA was present in intestinal biopsy specimens from healthy subjects and patients with CD but was elevated only in active UC. EBI3 levels in UC specimens correlated with histological scores of activity and T-cell infiltration. EBI3-positive cells that had a shape consistent with that of macrophages were identified in the lamina propria, and protein was detected by Western blotting. CONCLUSIONS: EBI3 is a novel IL-12-related cytokine that is expressed by macrophage-like cells in normal intestine and CD and has enhanced expression in active UC but not in active CD. 相似文献
15.
It is well known that a nonlinear system with a white Gaussian noise input can be characterized in terms of kernels using the celebrated Wiener theory. In a practical use of the method, however, one may encounter difficulty in obtaining higher order kernels except for the first few because of, for instance, the excessive computational requirement. In this paper, we give an integro-differential formula on the kernels and as its application, an algorithm to identify a cascade system of a linear, a memoryless nonlinear, and linear subsystems, which we call a sandwich system as a whole. According to the formula, kernels up to the second order for different power levels of the input noise are required to identify the subsystems. Impulse response functions of the two linear subsystems can be obtained under appropriate normalization conditions, while the nonlinear subsystem is estimated in the form of a truncated Hermite polynomial expansion. As illustrated examples, two such systems are identified using the algorithm. 相似文献
16.
17.
The main objective of this study is to devise a technique, which, when implemented into finite-element codes, is efficiently applicable to impact collapse analyses of framed structures. In this study, the formerly developed adaptively shifted integration (ASI) technique for the linear Timoshenko beam element is modified into the ASI-Gauss technique by placing the numerical integration points of the two consecutive elements forming an elastically deformed member in such a way that stresses and strains are evaluated at the Gaussian integration points of the two-element member. On comparison with the ASI technique, the ASI-Gauss technique proves its higher accuracy and efficiency in elastic range. Moreover, instead of applying impact loads in the form of nodal forces, we consider the impact phenomenon by means of contacts between the elements involved and the elemental contact algorithm is verified from the point of conservation of energy. Impact analyses considering member fracture with different sets of parameters are performed using a high-rise framed structure and a small aircraft. From the results obtained, we can observe propagation phenomena of impact loads and shock waves. Also, a proper difference in impact damage is obtained by different sets of parameters. The results also indicate that the mass of the aircraft has a stronger influence on impact damage than its velocity. Moreover, soon after impact, tensile stresses are observed in the columns that were compressed by dead loads before impact. 相似文献
18.
Lithium Batteries: Carbon‐Rich Active Materials with Macrocyclic Nanochannels for High‐Capacity Negative Electrodes in All‐Solid‐State Lithium Rechargeable Batteries (Small 25/2016) 下载免费PDF全文
19.
M Isobe Y Kumura Y Murata S Takaki A Tominaga K Takatsu Z Ogita 《Canadian Metallurgical Quarterly》1992,14(3):755-758
The chromosomal location of the human gene for the alpha subunit of interleukin-5 receptor (IL5RA) has been determined. The human IL5RA gene was localized to the short arm of chromosome 3 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines. The IL5RA gene has been further localized to human chromosome region 3p24-3p26 by in situ hybridization of a molecularly cloned IL5RA cDNA fragment to metaphase chromosomes. The results suggest that the IL5RA locus is unlinked to other members of the hematopoietic receptor family. Assignment of the IL5RA gene to chromosome 3 at bands p26-p24 raises the possibility that it may be altered by certain nonrandom chromosomal abnormalities arising in human hematopoietic malignancies and solid tumors. 相似文献
20.
Yasunori Imada Takafumi Shido Ryuichiro Ohnishi Kiyoshi Isobe Masaru Ichikawa 《Catalysis Letters》1996,38(1-2):101-108
SiO2-impregnated complete and incomplete cubane-type molybdenum oxide clusters such as [(RhCp*)4Mo4O16] (I) and [(RhCp*)2Mo3O9(OMe)4] (II) were photoreduced with a UHP-Hg lamp (> 365 nm) in CO, resulting in the formation of catalytically active species for propene metathesis at 300 K. The CO-photoreduced Mo oxide clusters were characterized by EXAFS, IR, XPS and TPD technique. The results suggest that the Mo-O4c (four-centered bridging oxygens) in I were specifically reduced with CO under the illumination to produce two sets of subcarbonyl species, e.g., those characteristic of the IR bands at 2061 and 2021 cm–1, which were thermally inactive for the13CO exchange reaction but very active under illumination at 300 K, possibly assignable to Mo(CO)x (x = 2, 3), whereas those at 2092 and 2035 cm–1 due to Rh(CO)2, which were readily exchangeable with13CO at 300 K in dark. Removal of both carbonyls attached on Rh and Mo in photoreduced I and II by evacuation at 375–440 K led to the formation of oxygen-deficient Mo4+/Mo5+ sites, which exhibited high catalytic activities in propene metathesis at 300 K to produce an equimolar mixture of ethene and 2-butenes. The CO-photoreduced incomplete cubane Mo oxide cluster (II) exhibited higher activities and higher trans/cis ratios of 2-butenes in the reaction, compared with those on the photoreduced I. 相似文献