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81.
    
To examine the effect of trehalose accumulation on response to saline stress in Saccharomyces cerevisiae, we constructed deletion strains of all combinations of the trehalase genes ATH1, NTH1 and NTH2 and examined their growth behaviour and intracellular trehalose accumulation under non‐stress and saline‐stress conditions. Saline stress was induced in yeast cells by NaCl addition at the exponential growth phase. All deletion strains showed similar specific growth rates and trehalose accumulation to their parent strain under non‐stress conditions. However, under the saline stress condition, one single deletion strain, nth1Δ, two double deletion strains, nth1Δ ath1Δ and nth1Δ nth2Δ, and the triple deletion strain nth1Δnth2Δ ath1Δ, all of which carry the nth1Δ deletion, showed increased trehalose accumulation as compared to the parent and other deletion strains. In particular, our statistical analysis revealed that the triple deletion strain showed a higher growth rate under the saline stress condition than the parent strain. Moreover, some deletion strains showed further trehalose accumulation under non‐stress conditions by overexpression of the TPS1 or TPS2 genes encoding the enzymes related to trehalose biosynthesis at the mid‐exponential phase. Such increased trehalose accumulation prior to NaCl addition could improve the growth of these strains under saline stress. Our results indicate that high trehalose accumulation prior to NaCl addition, rather than after NaCl addition, is necessary to achieve high growth activity under stress conditions. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   
82.
    
In Saccharomyces cerevisiae, Chs4p directly interacts with chitin synthase III (Chs3p) to act as a post-translational regulator of the Chs3p complex. We identified four Chs4p homologous proteins in Schizosaccharomyces pombe which we named Chr1, Chr2, Chr3 and Chr4 (putative chitin synthase regulatory factor). We assessed the functions of these proteins and found that while overproduction of Chr1, Chr2 or Chr3 did not affect the cellular morphology of wild-type Sz. pombe cells, overproduction of Chr4 caused the cells to form multi-septa and delayed their growth. All multiple disruptants of chr1, chr2, chr3 and chr4 grew normally under a variety of growth conditions. However, while chitin synthase II (Chs2) normally localizes exclusively at the septum, in many chr4-disrupted cells it was found in the cytoplasm and the septa. Chs2 did localize at the abnormal septa caused by the overproduction of chr4+. Chr4-13Myc expression was unaffected by the different media or growth conditions in both wild-type and the chs2 disruptant. Chs2 expression was also unaltered by the absence of Chr4. Moreover, Chr4-13Myc localized mostly at the tips and the septum during vegetative growth in chs2, chr1, chr2 and chr3 disruptants as well as in wild-type. Thus, chr4+ is involved in septum formation and is required for the proper localization of Chs2 at the septum in Sz. pombe.  相似文献   
83.
DNA microarray analysis was performed to examine the stress tolerance mechanism of a Saccharomyces cerevisiae recombinant strain exhibiting high trehalose accumulation and heat stress tolerance. Results suggest that the upregulation of sugar transporter genes is one of the key events for heat stress tolerance of the recombinant strain.  相似文献   
84.
    
Pr:LuAG thin film was deposited by pulsed laser deposition. Before and after thermal treatment, each film was characterized using X‐ray diffraction, scanning electron microscope, and cathode luminescence. Thermal treatment led this film to crystallization of Pr:LuAG with garnet structure. And ultra‐violet (UV) emission was obtained from the Pr:LuAG film pumped by irradiating electron beam. We applied this technique to produce a product of a UV light source using Pr:LuAG thin film target pumped by electron beam.  相似文献   
85.
Most studies use the facial expression to recognize a user’s emotion; however, gestures, such as nodding, shaking the head, or stillness can also be indicators of the user’s emotion. In our research, we use the facial expression and gestures to detect and recognize a user’s emotion. The pervasive Microsoft Kinect sensor captures video data, from which several features representing facial expressions and gestures are extracted. An in-house extensible markup language-based genetic programming engine (XGP) evolves the emotion recognition module of our system. To improve the computational performance of the recognition module, we implemented and compared several approaches, including directed evolution, collaborative filtering via canonical voting, and a genetic algorithm, for an automated voting system. The experimental results indicate that XGP is feasible for evolving emotion classifiers. In addition, the obtained results verify that collaborative filtering improves the generality of recognition. From a psychological viewpoint, the results prove that different people might express their emotions differently, as the emotion classifiers that are evolved for particular users might not be applied successfully to other user(s).  相似文献   
86.
Numerical calculations have been performed to elucidate unconventional electronic transport properties in disordered nanographene ribbons with zigzag edges (zigzag ribbons). The energy band structure of zigzag ribbons has two valleys that are well separated in momentum space, related to the two Dirac points of the graphene spectrum. The partial flat bands due to edge states make the imbalance between left- and right-going modes in each valley, i.e. appearance of a single chiral mode. This feature gives rise to a perfectly conducting channel in the disordered system, i.e. the average of conductance 〈g〉 converges exponentially to 1 conductance quantum per spin with increasing system length, provided impurity scattering does not connect the two valleys, as is the case for long-range impurity potentials. Ribbons with short-range impurity potentials, however, through inter-valley scattering, display ordinary localization behavior. Symmetry considerations lead to the classification of disordered zigzag ribbons into the unitary class for long-range impurities, and the orthogonal class for short-range impurities. The electronic states of graphene nanoribbons with general edge structures are also discussed, and it is demonstrated that chiral channels due to the edge states are realized even in more general edge structures except for armchair edges.  相似文献   
87.
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Appropriate monitoring and maintenance become important as an oil‐immersed transformer ages. Generally, dissolved gas analysis (DGA) is effective for the online diagnosis, and if an abnormality is detected, frequency response analysis (FRA) is effective for the offline diagnosis to localize the failed part. FRA diagnosis detects a slight change of waveforms; however, external factors may influence the results. Here, we discuss the influence of an insulating oil presence, tap position, and measurement lead wire length as possible parameters that influence the FRA characteristics. We confirmed that the measurement parameters examined here greatly influenced the FRA characteristics. In the actual diagnosis, it is important to remove these external parameters as much as possible, and keep a detailed record of the measurement parameters. © 2013 Wiley Periodicals, Inc. Electr Eng Jpn, 186(1): 18–25, 2014; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.22313  相似文献   
88.
为研究多孔隙岩石的变形特性,提出一个适用于多孔隙岩石的非线形流变模型(本构方程).本模型是由弹簧与阻尼器串连组成,弹簧部分采用作者曾经提出的本构方程;代表黏性部分的阻尼器设定加载前黏度很小,随着荷载的增加而逐渐加大.在低应力水平下的蠕变试验中,由于弹性系数没有明显的下降,因而设定阻尼器的应变与蠕变应变相同.为验证模型的准确性,对泥质砂岩、大谷凝灰岩、田下凝灰岩与河津凝灰岩4类岩石分别进行理论计算与试验测试.对于各类岩石,计算得到的应力-应变曲线与实测曲线基本一致,而且用此流变模型,发现气干与湿润状态下杨氏模量具有明显的差异.研究结果表明,在这2种状态下,杨氏模量的差异是由于非弹性应变(即阻尼器的应变)不同而引起的.在湿润状态下,阻尼器的应变增加越大,杨氏模量则变得越小.即使在较低的应力条件下,阻尼器的应变比以往研究中所认为的应变要大很多.  相似文献   
89.
自适应光学技术能够校正大气湍流造成的光束畸变,但常规自适应光学系统需要波前传感器测量波前信息.在本文中系统不使用波前传感器,而是以图像清晰度作为优化指标,使用无模型的随机并行梯度优化算法,对大气湍流引起的波前误差进行时实补偿,结果表明此系统算法经过多次迭代后达到很好的校正效果.  相似文献   
90.
It is important to detect a structural change in a time series quickly as a trigger to remodeling the forecasting model. The well-known Chow test has been used as the standard method for detecting change, especially in economics. However, we have proposed the application of the sequential probability ratio test (SPRT) for detecting the change in single-regression modeled time-series data. In this article, we show experimental results using SPRT and the Chow test when applied to time-series data that are based on multiple regression models. We also clarify the effectiveness of SPRT compared with the Chow test in its ability to detect change early and correctly, and its computational complexity. Moreover, we extend the definition of the point at which structural change is detected with the SPRT method, and show an improvement in the accuracy of change detection.  相似文献   
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