Determination of hyoscyamine and scopolamine in serum and urine of humans by liquid chromatography with tandem mass spectrometry

A simple method was developed for the analysis of hyoscyamine and scopolamine in human serum and urine using liquid chromatography with tandem mass spectrometry (LC/MS/MS). Hyoscyamine and scopolamine in serum and urine were cleaned up with an Oasis HLB cartridge and a PSA cartridge. The LC separation was carried out on an ODS column, using linear gradient elution with 5 mmol/L IPCC-MS3-methanol as the mobile phase. The mass spectral acquisition was done in the positive ion mode by applying selected reaction monitoring (SRM). The recoveries of hyoscyamine and scopolamine were 86.0-105% from human serum and urine fortified at 0.2 ng/mL and 10 ng/mL. The detection limits of hyoscyamine and scopolamine were 0.02 ng/mL. Four serum and three urine samples of humans poisoned by eating Datura innoxia Mill. were analyzed by this method. Hyoscyamine and scopolamine were detected at the levels of 0.45-3.5 ng/mL in all serum samples and 170-670 ng/mL in all urine samples.     

Motional coherency in chain dynamics of polybutadiene studied by molecular dynamics simulations

Molecular dynamics simulations of 1,4-polybutadiene in bulk amorphous state were performed. Results were compared with the recent neutron spin-echo measurements. To investigate motional coherency the relaxation rates for the collective and self-motions, the collective and self-relaxation rates, were evaluated for the short and long time regimes of the normalized intermediate scattering functions. The scattering vector dependence of the collective relaxation rates estimated for both fast and slow processes indicated a minimum at scattering vector q = 1.5 Å⁻¹, corresponding to the position of a peak in the static structure factor. The self-relaxation rates increased monotonously with q. A phenomenon known as de Gennes narrowing was reproduced well in the simulation and found to be originated from the inter-molecular correlation. The collective relaxation rate evaluated for fast process appeared to modulate around a peak of q = 2.9 Å⁻¹, corresponding to the intra-molecular correlation.     

SST estimation within Syowa's station mask using MOS-1's visible and thermal infrared radiometers

Since February 1989 measurements from MOS-1 could be received at Syowa Station in Antarctica. The first set of MOS-1 visible and thermal infrared (VTIR) recordings from Syowa Station is analysed in this study. A method developed by Schüssel and Grassl is applied for ice and cloud filtering. For clear ocean pixels the sea surface temperatures are estimated by applying Muneyama's split window algorithm. A sea surface temperature composite of ten VTIR passes is presented.     

Characteristics of porous polymer composite columns prepared by radiation cast-polymerization

Porous polymer composite columns having porous structure were prepared by radiation cast-polymerization of hydrophilic monomers at low temperature and their characteristics were studied. The porosity of the polymer increased with decreasing monomer concentration. The elution time of water in the polymer column increased with increasing monomer concentration and with decreasing irradiation temperature. The elution time was dependent on the degree of hydration of the polymer. The polymer with a degree of hydration of 0.2 to 0.4 gave the minimum elution time. The elution time decreased with the addition of porous inorganic substances.     

Mie scattering from sphere structures in polymer blends

Polymer blends of transparent poly(methyl methacrylate) and polystyrene become opaque due to light scattering at the boundaries of the two polymers. The polymer blend is light brown when it is illuminated by white light. The coloring depends on the spherical domain structures existing in the polymer blend. The coloring was analyzed by using the rigorous Mie theory. The Mie results were compared with the semiempirical results previously reported by the authors. The wavelength dependence of theoretical scattering efficiencies on radii of scattering spheres from 0.05 to 1.2 μm was obtained for polystyrene spheres in poly(methyl methacrylate) matrix, and vice versa. The scattering at the short wavelength region is stronger than at the long wavelength region. The scattering efficiencies become almost constant in the visible wavelength region for sufficiently large spheres.     

Pose-Invariant Facial Expression Recognition Using Variable-Intensity Templates

In this paper, we propose a method for pose-invariant facial expression recognition from monocular video sequences. The advantage of our method is that, unlike existing methods, our method uses a simple model, called the variable-intensity template, for describing different facial expressions. This makes it possible to prepare a model for each person with very little time and effort. Variable-intensity templates describe how the intensities of multiple points, defined in the vicinity of facial parts, vary with different facial expressions. By using this model in the framework of a particle filter, our method is capable of estimating facial poses and expressions simultaneously. Experiments demonstrate the effectiveness of our method. A recognition rate of over 90% is achieved for all facial orientations, horizontal, vertical, and in-plane, in the range of ±40 degrees, ±20 degrees, and ±40 degrees from the frontal view, respectively. Electronic Supplementary Material  The online version of this article () contains supplementary material, which is available to authorized users.     

Synthesis of sulfonated organic–inorganic hybrids through the radical copolymerization of vinyl sulfonate esters and vinyl trialkoxysilanes

Novel organic–inorganic hybrids with sulfonic acid groups were prepared using random copolymers composed of vinyl sulfonate esters and vinyl trialkoxysilanes. Five vinyl sulfonate esters with different substituent groups were employed as protecting monomers for the production of the poly(vinyl sulfonic acid) component, and three vinyl trialkoxysilanes were used as cross-linkable monomers. Free radical and reversible addition-fragmentation chain transfer (RAFT) copolymerizations were performed for the production of random copolymers with two different functional groups. The selective deprotection of the sulfonate esters of the copolymers proceeded smoothly and resulted in the formation of copolymers with lithium vinyl sulfonate units and cross-linkable trialkoxysilane units. The co-condensation of the trialkoxysilane moieties in the deprotected copolymers with cross-linkers yielded transparent hybrid films that contained lithium sulfonate groups without aromatic rings or ester linkages.     

FLOW IN AGITATED THIN FILM HORIZONTAL EVAPORATOR

Abstract

We made a theoretical analysis for an axial flow of a working liquid of low viscosity without concern for the thermal effects. Variables studied were rotational speed of wiping blades, volumetric flow rate and viscosity.

When the viscosity is low, theoretical values are in proper accord with experimental ones. As a result, we can say that the residence time and the effective area for heat transfer are able to be controlled (when the viscosity is low) by selecting the rotational speed.     

Environmental stress cracking of poly(acrylonitrile-butadiene-styrene)

The environmental stress cracking (ESC) of acrylonitrile-butadiene-styrene (ABS) copolymer caused by a non-ionic surfactant (poly-oxyethylene alkylether) was studied by constant-load tensile creep tests and edge crack tension (ECT) tests. The fracture surfaces were investigated by a scanning electron microscope (SEM) and the morphology of the crack tip was investigated by a transmission electron microscope (TEM). It was found that the results of the creep tests performed in the non-ionic surfactant were very different from those performed in air. SEM images of the fracture surfaces showed that there were three different mechanisms of fracture and that specimens had a tendency to rupture by ESC when the stress was small. The results of the ECT tests and the TEM images showed that the change in the mechanism of the fracture was attributable to the change of morphology at the crack tip.     

Development of Arm Equipped Single Wheel Rover: Effective Arm-Posture-Based Steering Method

We have been proposing a new planetary rover system named SMC Rover. This system consists of one main body and detachable units, which can work as child rovers, and also become driving units of the main body. Each detachable unit named Uni-Rover consists of a single manipulator mounted on a wheel. Prototype models of the Uni-Rover and the SMC Rover have been already developed. This paper describes a new method of trajectory modeling for the Uni-Rover using the relation between arm posture and turning radius. At first, an intuitively recognizable model which uses zero slip angle is introduced. However, because this intuitive model has some problems, a new solution which considers the loss of friction on the wheel and on the caster is also discussed. The validity of the presented method is verified by experiments with an actual mechanical model. Moreover, an effective steering method which considers the margin of the energy stability is introduced.Kazuhiro Motomura is a Ph.D candidate at the Tokyo Institute of Technology (Tokyo Tech). In 2001 he was admitted to skip the fourth grade of the bachelor course in the Department of Mechano-Aerospace engineering at Tokyo Tech to enterthe master course in the Department of Mechanical and Aerospace Engineering at the same Institute. He received the B.E. degree in National Institution for Academic Degrees an University Evaluation in 2002, and received the M.E. degree in the Department of Mechanical and Aerospace Engineering at Tokyo Tech in 2003. He has been a research fellow of the Japan Society for the Promotion of Science from 2004. He awarded the Incentive Award for the presentation in the 17th RSJ Annual Conference in 2001, and the Finalists for the Best Video Award in the IEEE International Conference on Robotics and Automation in 2003. His research interests include design of robotic mechanisms and control of mobile robots. He studies about the development of planetary rovers.Atsushi Kawakami awarded the chance to skip fourth gradein the bachelor course of Department of Mechano-Aerospace engineering in Tokyo Institute of Technology, and entered the master course in 1997. He received the M.E. and Dr. E. degrees in Department of Mechanical Engineering Science in Tokyo Institute of Technology in 1999 and 2003, respectively. From 2003, he is a post-doctoral researcher. His research interest is in design of robotic mechanisms. He studies about the development of planetary rover. He awarded the Incentive Award for the presentation in the 17th RSJ Annual Conference in 2001, and the Finalists for the Best Video Award in the IEEE International Conference on Robotics and Automation in 2003. He also acts as an organizer and lecturerin several hands on robots competitions that aim at the promotion and education for the beginners.Shigeo Hirose was born in Tokyo in 1947. He received the B. E. degree with first class honors in Mechanical Engineering from Yokohama National University in 1971, and the M. E. and Dr. E. degrees in Control Engineering from the Tokyo Institute of Technology in 1973 and 1976, respectively. From 1976 to 1979 he was a Research Associate, from 1979 to 1992 an Associate Professor and since 1992 he has been a Professor in the Department of Mechanical and Aerospace Engineering at the Tokyo Institute of Technology. He is the Fellow of both IEEE and Japanese Society of Mechanical Engineers. His research interest is in design of novel robotic mechanisms and its control. He awarded more than 30 prizes from academic societies; these include the first Pioneer in Robotics and Automation Award in 1999 and Best Conference Paper Award in 1995 both from IEEE Robotics & Automation Society. Prof. Hirose has published more than 200 academic papers as well as several books, including Snake Inspired Robots (Kogyo-chosakai Publishing Co. Ltd, 1987, in Japanese), Robotics (Shokabo Publishing Co. Ltd., 1987, in Japanese), and Biologically Inspired Robots (Oxford University Press,1993).