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71.
Kazuo Hasue Shoji Nakahara Jun Morimoto Tetsuo Yamagami Yoichi Okamoto Toru Miyakawa 《Propellants, Explosives, Pyrotechnics》1995,20(4):187-191
To find an effective laser source to ignite energetic materials, the absorption spectra of some energetic materials are obtained by means of a photoacoustic spectroscopy (PAS). In this experiment, PAS covers the wavelength region of 400 nm-1600 nm in which no other conventional method can take absorption spectra for powdered energetic materials. Photoacoustic spectra of 18 energetic materials are reported. In general, energetic materials tested showed peaks in 600 nm–800 nm and 1400 nm–1600 nm ranges. It is found that the energy required to initiate explosives in the case of ruby laser initiation were correlated with their photoacoustic signal intensities. 相似文献
72.
Takanori Furuta Ichiro Nakagawa Shohei Yokoyama Yudai Morisaki Yasuhiko Saito Hiroyuki Nakase 《International journal of molecular sciences》2022,23(7)
Mitochondrial membrane potential regulation through the mitochondrial permeability transition pore (mPTP) is reportedly involved in the ischemic postconditioning (PostC) phenomenon. Melatonin is an endogenous hormone that regulates circadian rhythms. Its neuroprotective effects via mitochondrial melatonin receptors (MTs) have recently attracted attention. However, details of the neuroprotective mechanisms associated with PostC have not been clarified. Using hippocampal CA1 pyramidal cells from C57BL mice, we studied the involvement of MTs and the mPTP in melatonin-induced PostC mechanisms similar to those of ischemic PostC. We measured changes in spontaneous excitatory postsynaptic currents (sEPSCs), intracellular calcium concentration, mitochondrial membrane potential, and N-methyl-D-aspartate receptor (NMDAR) currents after ischemic challenge, using the whole-cell patch-clamp technique. Melatonin significantly suppressed increases in sEPSCs and intracellular calcium concentrations. The NMDAR currents were significantly suppressed by melatonin and the MT agonist, ramelteon. However, this suppressive effect was abolished by the mPTP inhibitor, cyclosporine A, and the MT antagonist, luzindole. Furthermore, both melatonin and ramelteon potentiated depolarization of mitochondrial membrane potentials, and luzindole suppressed depolarization of mitochondrial membrane potentials. This study suggests that melatonin-induced PostC via MTs suppressed the NMDAR that was induced by partial depolarization of mitochondrial membrane potential by opening the mPTP, reducing excessive release of glutamate and inducing neuroprotection against ischemia-reperfusion injury. 相似文献
73.
Takashi Kitaguchi Yasumitsu Ogra Yuji Iwashita Kazuo T. Suzuki 《European Food Research and Technology》2008,227(5):1455-1460
Buckwheat (Fagopyrum esculentum Moench) and quinoa (Chenopodium quinoa Willdenow) are widely used as food ingredients. The nutritional characteristics of these plants, i.e., high contents of proteins
and amino acids suggest that selenium (Se) is preserved as selenoamino acid derivatives, in particular, selenomethionine (SeMet)
in proteins, similar to selenized yeast. Therefore, buckwheat and quinoa are expected to be a good nutritional source of Se.
Selenized buckwheat and quinoa were cultivated on Se-fortified soil using sparingly soluble Se salts, such as barium selenate
and barium selenite. Se concentration in the edible parts of these plants was determined, and Se extraction efficiency with
enzyme or alkali was evaluated. In addition, the chemical species of Se in the low molecular weight fraction of these plants
were determined by HPLC-ICP-MS. Total Se concentrations in the edible parts of selenized buckwheat and quinoa were 170.4 ± 2.9 μg/g
and 102.7 ± 2.4 μg/g wet weight, respectively. Thus, these selenized seeds were found to be Se accumulators. The results indicate
that Se in selenized buckwheat exists mainly as SeMet, while Se in selenized quinoa exists not only as SeMet but also as selenate
(Se(VI)) and non-protein forms. 相似文献
74.
Involvement of Anthocyanins and other Phenolic Compounds in Radical-Scavenging Activity of Purple-Fleshed Sweet Potato Cultivars 总被引:7,自引:0,他引:7
T. Oki M. Masuda s. Furuta y. Nishiba N. Terahara I. Suda 《Journal of food science》2002,67(5):1752-1756
ABSTRACT: The 80% ethanol extracts of 5 purple-fleshed sweet potato cultivars were separated into 2 fractions, anthocyanins- and phenolic compounds-rich fractions, to clarify the contribution of these constituents to the radical-scavenging activity. The separation was accomplished with an ethyl acetate liquid/liquid extraction. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity in each fraction and the contributors varied according to the cultivars. The dominant DPPH radical-scavengers in "Ayamurasaki" and "Kyushu-132" were anthocyanins rather than phenolic compounds, while those in "Miyanou-36" and "Bise" were phenolic compounds, such as chlorogenic acid. Furthermore, the high-performance liquid chromatography analysis of anthocyanins showed that "Ayamurasaki" and "Kyushu-132" were rich in anthocyanins with peonidin aglycon, whereas "Miyanou-36,""Bise," and "Tanegashimamurasaki" contained cyanidin aglycon. 相似文献
75.
Kinetic Study of the Prooxidant Effect of α-Tocopherol. Hydrogen Abstraction from Lipids by α-Tocopheroxyl Radical 总被引:1,自引:0,他引:1
Aya Ouchi Masaharu Ishikura Kensuke Konishi Shin-ichi Nagaoka Kazuo Mukai 《Lipids》2009,44(10):935-943
A kinetic study of the prooxidant effect of α-tocopherol was performed. The rates of allylic hydrogen abstraction from various
unsaturated fatty acid esters (ethyl stearate 1, ethyl oleate 2, ethyl linoleate 3, ethyl linolenate 4, and ethyl arachidonate 5) by α-tocopheroxyl radical in toluene were determined, using a double-mixing stopped-flow spectrophotometer. The second-order
rate constants (k
p) obtained are <1 × 10−2 M−1 s−1 for 1, 1.90 × 10−2 M−1 s−1 for 2, 8.33 × 10−2 M−1 s−1 for 3, 1.92 × 10−1 M−1 s−1 for 4, and 2.43 × 10−1 M−1 s−1 for 5 at 25.0 °C. Fatty acid esters 3, 4, and 5 contain two, four, and six –CH2– hydrogen atoms activated by two π-electron systems (–C=C–CH2–C=C–). On the other hand, fatty acid ester 2 has four –CH2– hydrogen atoms activated by a single π-electron system (–CH2–C=C–CH2–). Thus, the rate constants, k
abstr/H, given on an available hydrogen basis are k
p/4 = 4.75 × 10−3 M−1 s−1 for 2, k
p/2 = 4.16 × 10−2 M−1 s−1 for 3, k
p/4 = 4.79 × 10−2 M−1 s−1 for 4, and k
p/6 = 4.05 × 10−2 M−1 s−1 for 5. The k
abstr/H values obtained for 3, 4, and 5 are similar to each other, and are by about one order of magnitude higher than that for 2. From these results, it is suggested that the prooxidant effect of α-tocopherol in edible oils, fats, and low-density lipoproteins
may be induced by the above hydrogen abstraction reaction. 相似文献
76.
Mutational analysis of structure--activity relationships in human tumor necrosis factor-alpha 总被引:1,自引:0,他引:1
Yamagishi Jun-ichi; Kawashima Hitoshi; Matsuo Noriyuki; Ohue Mayumi; Yamayoshi Michiko; Fukui Toshikazu; Kotani Hirotada; Furuta Ryuji; Nakano Katsuji; Yamada Masaaki 《Protein engineering, design & selection : PEDS》1990,3(8):713-719
To determine the region of human tumor necrosis factor-alpha(TNF-), essential for cytotoxic activity against mouse L-M cells,single amino-acid-substituted TNF- mutant proteins (muteins)were produced in Escherichia coli by protein engineering techniques.An expression plasmid for TNF- was mutagenized by passage throughan E.coli mutD5 mutator strain and by oligonucleotide-directedmutagenesis. Approximately 100 single amino-acid-substitutedTNF- muteins were produced and assayed for cytotoxic activity.The cytotoxic activities of purified TNF- muteins, e.g. TNF-31T,-32Y, -82D, -85H, -115L, -141Y, -144K and -146E, were < 1%of that of parent TNF-. These results indicate that the integrityof at least four distinct regions of the TNF- molecule is requiredfor full biological activity. These regions are designated asfollows: region I, from position 30 to 32; region II, from position82 to 89; region III, from position 115 to 117; region FV, fromposition 141 to 146. In addition, TNF-141Y could not completelycompete with parent TNF- for binding to the receptor. This demonstratesthat region IV, and at least aspartk acid at position 141, mustbe involved in the TNF receptor binding site. 相似文献
77.
Yusuke Furuta Hiroyuki Nishikawa Takahiro Satoh Yasuyuki Ishii Tomihiro Kamiya Ryota Nakao Satoshi Uchida 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2009,267(12-13):2285-2288
Fabrication of high-aspect-ratio microstructures was performed by proton beam writing (PBW) using a microbeam line at Takasaki Ion Accelerators for Advanced Radiation Application (TIARA), JAEA Takasaki, JAPAN. As one of the applications of the high-aspect-ratio structures micro-machined by PBW, we utilized the high-aspect pillars for electric-micro filters of microbes such as Escherichia coli and Yeast based on the dielectrophoretic force. The filter is equipped with high-aspect pillars with a height of ~20 μm and a diameter of ~1 μm on a glass plate. Evaluation of the dielectrophoresis (DEP) device for capturing E. coli and Yeast was made using either observation by optical microscope or photoluminescence (PL) measurements. 相似文献
78.
Yoshihiko Hibi Katsuyuki Fujinawa Seiji Nishizaki Kazuo Okamura Masaharu Tasaki 《Soils and Foundations》2010,50(1):143-159
Diffusion, dispersion, and advection are important processes in multi-gas systems in soils. To date, both Fick's model and the Dusty Gas (DG) model have been used to model the movement of gases in these systems. Dispersion is included in the dispersion-advection equation with Fick's Model for the movement of gases in gas-phase of soil, yet the movement of gases in multi-component gas-soil systems is considered to be expressed more accurately by the DG model than by Fick's model. However to date, no study has investigated the necessity of considering dispersion in the Dusty Gas (DG) model. We carried out column experiments for nitrogen-methane, nitrogen-carbon dioxide, and carbon dioxide-methane binary gas systems in sandy soil, and also did simulations on the same systems using both Fick's model and the DG model. A comparison of the results of the column experiments with our simulations confirmed that there was no need to consider the dispersion in the advection-diffusion equations with the DG model when the velocity of gas was 0.05-0.4 cm/s in Toyoura sand. Furthermore, our experiments and simulations with the DG model showed that, rather than dispersion, tortuosity should be taken into account in application of the DG model to the above condition. 相似文献
79.
80.