Improved bioabsorbability of synthetic hydroxyapatite through partial dissolution-precipitation of its surface

Even though synthetic hydroxyapatite (HAp) has a chemical composition similar to the mineral phase of bone, it is minimally absorbed and replaced by bone tissue. This could be because HAp is composed of compactly arranged apatite crystals with homogenously large grains. In this study, the surface and non-stoichiometry of the synthetic HAp crystals was modified by partial dissolution and precipitation (PDP) to improve bioabsorbability of HAp. In vitro cell culture demonstrated that more osteoclasts were activated on PDP-HAp compared with HAp. In vivo implantation using a rabbit bone defect model revealed that PDP-HAp was gradually degraded and was replaced by bone tissue. Consistent with the in vitro results, more osteoclasts were activated in PDP-HAp than in HAp, indicating that the former was absorbed through the stimulation of osteoclastic activity. These results suggest that the PDP technique may have clinical utility for modifying synthetic HAp for use as superior bone graft substitutes.     

Combination of Runoff Simulation with Sediment Modeling in Rivers and Its Application in the Lower Yellow River

Abstract

Previous research on runoff show flow and sediment movement in rivers, and river bed variations are usually separated. This paper, from the point of view of the basin as a whole system, presents an integrated approach by combining runoff simulation with numerical model of sediment transport in rivers to simulate the whole processes of hydrological variables and flow-caused bed variations from the very upper part of the basin to the river mouth. To accomplish this purpose, BTOPMC, a rainfall-runoff model, and NUSTM-1D, a well-developed numerical model for sediment transport and river bed variations, are selected to form a combined model. The application of the proposed model to the lower Yellow River shows that it can properly simulate rainfall-caused runoff, the change of suspended sediment concentration along the river, and river bed variation.     

Myristoylation of hippocalcin is linked to its calcium-dependent membrane association properties

Hippocalcin, a recently identified Ca(2+)-binding protein of the recoverin family exclusively expressed in the hippocampus, has a primary structure containing three putative Ca(2+)-binding sites (EF-hands) and a possible NH2-terminal myristoylation site. 45Ca blots demonstrated that every three EF-hand domains, expressed as fusion proteins in Escherichia coli, bind Ca2+, indicating that hippocalcin binds 3 mol of Ca2+/mol of protein. To determine whether hippocalcin is myristoylated, hippocalcin mRNA was translated in vitro in the presence of [3H]myristic acid. 3H label was resistant to hydroxylamine treatment, and replacement of NH2-terminal glycine with alanine prevented 3H label incorporation, indicating that in vitro translated hippocalcin covalently bound [3H]myristic acid at the NH2-terminal glycine. In vitro translated hippocalcin is quantitatively myristoylated, as evidenced by an electrophoretic mobility shift of [35S]methionine-labeled protein on two-dimensional gels. Native hippocalcin comigrated precisely with the in vitro translated hippocalcin on two-dimensional gels, suggesting that native hippocalcin is myristoylated. Native and in vitro translated hippocalcins, but not non-myristoylated mutagenic (Gly1-Ala1) hippocalcin, displayed Ca(2+)-dependent membrane association, indicating that myristoylation participates in its Ca(2+)-dependent membrane association properties. In vitro translated hippocalcin bound to phospholipid vesicles somewhat, however, phospholipid association was insufficient for its membrane association properties, suggesting that the NH2-terminal myristoyl moiety on hippocalcin interacts with lipid bilayers and facilitates interaction with other membrane proteins.     

Evaluation of fatigue crack growth behavior of GLARE3 fiber/metal laminates using a compliance method

The objectives of this study were to investigate the effectiveness of a compliance method for analyzing the fatigue crack growth of GLARE3 fiber/metal laminates. The materials tested were GLARE3-5/4 (2.6 mm thick) and GLARE3-3/2 (1.4 mm thick). Centrally notched specimens with two kinds of notch length and two kinds of fiber orientation were fatigue tested under constant amplitude loading. The expression of the experimental stress intensity factor, K_exp, for the 2024-T3 aluminum-alloy layers of a GLARE3 is formulated and K_exp were obtained from the relationship between crack length and specimen compliance. The test results clarified the following: (1) da/dN–ΔK_exp relationships roughly show the linear relationship independent of the maximum stress level, specimen thickness, notch length, and fiber orientations, (2) the da/dN–ΔK_exp relationships approximately agree with the linear part and its extension of Paris–Erdogan’s law obtained for the da/dN–ΔK relationship of the 2024-T3 aluminum-alloy, (3) the compliance method is effective for analyzing fatigue crack growth in GLARE3 laminates.     

Reduction of lipophilicity at the lipophilic domain of RXR agonists enables production of subtype preference: RXRalpha-preferential agonist possessing a sulfonamide moiety

Retinoid X receptor agonists (RXR agonists, rexinoids) are interesting candidates for the treatment of cancers such as tamoxifen-resistant breast cancer and taxol-resistant lung cancer. However, well-known RXR agonists possess a strong lipophilic character. In addition, although RXR has three subtypes, no subtype-selective RXR agonists are known. Thus we aimed to produce less-lipophilic and subtype-selective RXR agonists. By designing sulfonamide-type RXR agonists, 4-[N-methanesulfonyl-N-(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-2-naphthyl)amino]benzoic acid (8 a) was found to prefer RXRalpha over RXRbeta and RXRgamma, although the potency is less than the potencies of well-known RXR pan-agonists. Moreover, our results suggest that the reduction of lipophilicity at the hydrophobic interaction region of RXR agonists enables production of RXR subtype preference. Our finding will be useful for the creation of more potent and less-lipophilic subtype-selective RXR agonists aimed at the reduction of undesirable side effects.     

Auto-resonant eniotron oscillator using a magnetron type cavity

In an ideal auto-resonant eniotron oscillator (ARPO), all the electrons are able to give almost all their kinetic energy to an electromagnetic wave, resulting in a conversion efficiency of nearly 100%. However, the electric field strength required to drive a highly efficient interaction is difficult to achieve in an ordinary rectangular or circular waveguide cavity because of the low interaction coupling between electrons and the electromagnetic wave in the cavity. In this aper, we describe our investigation of an ARPO with a magnetron type cavity in lace of that above. In this cavity, the coupling becomes sufficiently strong to confirm experimentally the romising features of an ARPO. Additionally, the experiments should be useful for developing highly efficient medium-power oscillators in the microwave region. Here we resent our rocedure for designing such an experimental tube and our computer simulation results for its operational characteristics