A taxonomy of variability realization techniques

Development of software product families relies heavily on the use of variability to manage the differences between products by delaying design decisions to later stages of the development and usage of the constructed software systems. Implementation of variability is not a trivial task, and is governed by a number of factors. In this paper, we describe the factors that are relevant in determining how to implement variability, and present a taxonomy of variability realization techniques. Copyright © 2005 John Wiley & Sons, Ltd.     

Design of a General‐Purpose European Compound Screening Library for EU‐OPENSCREEN

This work describes a collaborative effort to define and apply a protocol for the rational selection of a general‐purpose screening library, to be used by the screening platforms affiliated with the EU‐OPENSCREEN initiative. It is designed as a standard source of compounds for primary screening against novel biological targets, at the request of research partners. Given the general nature of the potential applications of this compound collection, the focus of the selection strategy lies on ensuring chemical stability, absence of reactive compounds, screening‐compliant physicochemical properties, loose compliance to drug‐likeness criteria (as drug design is a major, but not exclusive application), and maximal diversity/coverage of chemical space, aimed at providing hits for a wide spectrum of drugable targets. Finally, practical availability/cost issues cannot be avoided. The main goal of this publication is to inform potential future users of this library about its conception, sources, and characteristics. The outline of the selection procedure, notably of the filtering rules designed by a large committee of European medicinal chemists and chemoinformaticians, may be of general methodological interest for the screening/medicinal chemistry community. The selection task of 200K molecules out of a pre‐filtered set of 1.4M candidates was shared by five independent European research groups, each picking a subset of 40K compounds according to their own in‐house methodology and expertise. An in‐depth analysis of chemical space coverage of the library serves not only to characterize the collection, but also to compare the various chemoinformatics‐driven selection procedures of maximal diversity sets. Compound selections contributed by various participating groups were mapped onto general‐purpose self‐organizing maps (SOMs) built on the basis of marketed drugs and bioactive reference molecules. In this way, the occupancy of chemical space by the EU‐OPENSCREEN library could be directly compared with distributions of known bioactives of various classes. This mapping highlights the relevance of the selection and shows how the consensus reached by merging the five different 40K selections contributes to achieve this relevance. The approach also allows one to readily identify subsets of target‐ or target‐class‐oriented compounds from the EU‐OPENSCREEN library to suit the needs of the diverse range of potential users. The final EU‐OPENSCREEN library, assembled by merging five independent selections of 40K compounds from various expert groups, represents an excellent example of a Europe‐wide collaborative effort toward the common objective of building best‐in‐class European open screening platforms.     

ENDOW (efficient development of offshore wind farms): modelling wake and boundary layer interactions

While experience gained through the offshore wind energy projects currently operating is valuable, a major uncertainty in estimating power production lies in the prediction of the dynamic links between the atmosphere and wind turbines in offshore regimes. The objective of the ENDOW project was to evaluate, enhance and interface wake and boundary layer models for utilization offshore. The project resulted in a significant advance in the state of the art in both wake and marine boundary layer models, leading to improved prediction of wind speed and turbulence profiles within large offshore wind farms. Use of new databases from existing offshore wind farms and detailed wake profiles collected using sodar provided a unique opportunity to undertake the first comprehensive evaluation of wake models in the offshore environment. The results of wake model performance in different wind speed, stability and roughness conditions relative to observations provided criteria for their improvement. Mesoscale model simulations were used to evaluate the impact of thermal flows, roughness and topography on offshore wind speeds. The model hierarchy developed under ENDOW forms the basis of design tools for use by wind energy developers and turbine manufacturers to optimize power output from offshore wind farms through minimized wake effects and optimal grid connections. The design tools are being built onto existing regional‐scale models and wind farm design software which was developed with EU funding and is in use currently by wind energy developers. Copyright © 2004 John Wiley & Sons, Ltd.     

Confirmatory analysis of steroids in muscle using liquid chromatography-tandem mass spectrometry

A method is described for screening and confirmation of synthetic and endogenous steroids in muscle tissue. The method is sensitive, selective, and rapid and the consumption of organic solvents is low, compared to previously published methods. The procedure involves hydrolysis, defattening with heptane and final clean up with SPE using C(18) cartridge. After filtration, the analytes are analysed by LC/MS/MS and quantification is performed using deuterated internal standards. Decision limits (CCα) varied from 0.02 to 0.33 μg kg(-1) and the detection capabilities (CCβ) were <0.50 μg kg(-1). The mean within-laboratory reproducibility ranged 5-22% (%RSD(IR)). Endogenous steroids (e.g. testosterone, epitestosterone and androstenedione) have been included in the method, to provide an insight into their levels, as the presence of these steroids was detected several times during analysis of imported meat.     

Photoswitchable Superabsorbency Based on Nanocellulose Aerogels

Chemical vapor deposition of a thin titanium dioxide (TiO₂) film on lightweight native nanocellulose aerogels offers a novel type of functional material that shows photoswitching between water‐superabsorbent and water‐repellent states. Cellulose nanofibrils (diameters in the range of 5–20 nm) with native crystalline internal structures are topical due to their attractive mechanical properties, and they have become relevant for applications due to the recent progress in the methods of their preparation. Highly porous, nanocellulose aerogels are here first formed by freeze‐drying from the corresponding aqueous gels. Well‐defined, nearly conformal TiO₂ coatings with thicknesses of about 7 nm are prepared by chemical vapor deposition on the aerogel skeleton. Weighing shows that such TiO₂‐coated aerogel specimens essentially do not absorb water upon immersion, which is also evidenced by a high contact angle for water of 140° on the surface. Upon UV illumination, they absorb water 16 times their own weight and show a vanishing contact angle on the surface, allowing them to be denoted as superabsorbents. Recovery of the original absorption and wetting properties occurs upon storage in the dark. That the cellulose nanofibrils spontaneously aggregate into porous sheets of different length scales during freeze‐drying is relevant: in the water‐repellent state they may stabilize air pockets, as evidenced by a high contact angle, in the superabsorbent state they facilitate rapid water‐spreading into the aerogel cavities by capillary effects. The TiO₂‐coated nanocellulose aerogels also show photo‐oxidative decomposition, i.e., photocatalytic activity, which, in combination with the porous structure, is interesting for applications such as water purification. It is expected that the present dynamic, externally controlled, organic/inorganic aerogels will open technically relevant approaches for various applications.     

Photoacoustic holographic imaging of absorbers embedded in silicone

Light absorbing objects embedded in silicone have been imaged using photoacoustic digital holography. The photoacoustic waves were generated using a pulsed Nd:YAG laser, λ=1064 nm, and pulse length=12 ns. When the waves reached the silicone surface, they were measured optically along a line using a scanning laser vibrometer. The acoustic waves were then digitally reconstructed using a holographic algorithm. The laser vibrometer is proven to be sensitive enough to measure the surface velocity due to photoacoustic waves generated from laser pulses with a fluence allowed for human tissue. It is also shown that combining digital holographic reconstructions for different acoustic wavelengths provides images with suppressed noise and improved depth resolution. The objects are imaged at a depth of 16.5 mm with a depth resolution of 0.5 mm.     

Comparison between infrared and Raman spectroscopic analysis of maturing rabbit cortical bone

The molecular composition of the organic and inorganic matrices of bone undergoes alterations during maturation. The aim of this study was to compare Fourier transform infrared (FT-IR) and near-infrared (NIR) Raman microspectroscopy techniques for characterization of the composition of growing and developing bone from young to skeletally mature rabbits. Moreover, the specificity and differences of the techniques for determining bone composition were clarified. The humeri of female New Zealand White rabbits, with age range from young to skeletally mature animals (four age groups, n = 7 per group), were studied. Spectral peak areas, intensities, and ratios related to organic and inorganic matrices of bone were analyzed and compared between the age groups and between FT-IR and Raman microspectroscopic techniques. Specifically, the degree of mineralization, type-B carbonate substitution, crystallinity of hydroxyapatite (HA), mineral content, and collagen maturity were examined. Significant changes during maturation were observed in various compositional parameters with one or both techniques. Overall, the compositional parameters calculated from the Raman spectra correlated with analogous parameters calculated from the IR spectra. Collagen cross-linking (XLR), as determined through peak fitting and directly from the IR spectra, were highly correlated. The mineral/matrix ratio in the Raman spectra was evaluated with multiple different peaks representing the organic matrix. The results showed high correlation with each other. After comparison with the bone mineral density (BMD) values from micro-computed tomography (micro-CT) imaging measurements and crystal size from XRD measurements, it is suggested that Raman microspectroscopy is more sensitive than FT-IR microspectroscopy for the inorganic matrix of the bone. In the literature, similar spectroscopic parameters obtained with FT-IR and NIR Raman microspectroscopic techniques are often compared. According to the present results, however, caution is required when performing this kind of comparison.     

The metallurgy,mechanics, modelling and assessment of dissimilar material brazed joints

At the heart of any procedure for modelling and assessing the design or failure of dissimilar material brazed joints there must be a basic understanding of the metallurgy and mechanics of the joint. This paper is about developing this understanding and addressing the issues faced with modelling and predicting failure in real dissimilar material brazed joints and the challenges still to be overcome in many cases. An understanding of the key metallurgical features of such joints in relation to finite element modelling is presented in addition to a study of the mechanics and stress state at an abrupt interface between two materials. A discussion is also presented on why elastic singularities do not exist based on a consideration of the assumption of an abrupt change in material properties and plasticity in the vicinity of the joint. In terms of modelling real dissimilar material brazed joints; there are several barriers to accurately capturing the stress state in the region of the joint and across the brazed layer and these are discussed in relation to a metallurgical study of a real dissimilar material brazed joint. However, this does not preclude using a simplified modelling approach with a representative braze layer in design and failure assessment away from the interface. In addition modelling strategies and techniques for assessing the various failure mechanisms of dissimilar material brazed joints are discussed. The findings from this paper are applicable to dissimilar material brazed joints found in a range of applications; however the references listed are primarily focussed on work in fusion research and development.     

Alkoxide route to La0.5Sr0.5CoO3 epitaxial thin films on SrTiO3

An all alkoxide based sol–gel route was investigated for preparation of epitaxial La_0.5Sr_0.5CoO₃ (LSCO) films on 100 SrTiO₃ (STO) substrates. Films with 20–30 to 80–100 nm thickness were prepared by spin-coating 0.2–0.6 M (metal) solutions on the STO substrates and heat treatment to 800 °C at 2 °C min^− 1, 30 min, in air. The films were epitaxial with a cube-on-cube alignment and the LSCO cell was strained to match the STO substrate of 3.905 Å closely; a and b = 3.894 Å and 3.897 Å for the 20–30 and 80–100 nm films, respectively. The c-axis was compressed to 3.789 Å and 3.782 Å for the 20–30 and 80–100 nm films, respectively, which resulted in an almost unchanged cell volume as compared to polycrystalline film and nano-phase powders prepared in the same way. The SEM study showed mainly very smooth, featureless surfaces, but also some defects. A film prepared in the same way on an -Al₂O₃ substrate was dense and polycrystalline with crystallite sizes in the range 10–50 nm and gave cubic cell dimensions of a_c = 3.825 Å. The conductivity of the ca 30–40 nm thick polycrystalline film was 1.7 mΩcm, while the epitaxial 80–100 nm film had a conductivity of around 1.9 mΩcm.