Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells

Pleural mesothelial cells (PMCs) play a central role in the progression of pleural fibrosis. As pleural injury progresses to fibrosis, PMCs transition to mesenchymal myofibroblast via mesothelial mesenchymal transition (MesoMT), and produce extracellular matrix (ECM) proteins including collagen and fibronectin (FN1). FN1 plays an important role in ECM maturation and facilitates ECM-myofibroblast interaction, thus facilitating fibrosis. However, the mechanism of FN1 secretion is poorly understood. We report here that myosin 5b (Myo5b) plays a critical role in the transportation and secretion of FN1 from human pleural mesothelial cells (HPMCs). TGF-β significantly increased the expression and secretion of FN1 from HPMCs and facilitates the close association of Myo5B with FN1 and Rab11b. Moreover, Myo5b directly binds to GTP bound Rab11b (Rab11b-GTP) but not GDP bound Rab11b. Myo5b or Rab11b knockdown via siRNA significantly attenuated the secretion of FN1 without changing FN1 expression. TGF-β also induced Rab11b-GTP formation, and Rab11b-GTP but not Rab11b-GDP significantly activated the actin-activated ATPase activity of Myo5B. Live cell imaging revealed that Myo5b- and FN1-containing vesicles continuously moved together in a single direction. These results support that Myo5b and Rab11b play an important role in FN1 transportation and secretion from HPMCs, and consequently may contribute to the development of pleural fibrosis.     

Molding of PBO fabric reinforced thermoplastic composite to achieve high fiber volume fraction

In fiber‐reinforced plastic materials, the fiber volume fraction is one of the most important parameters, and it strongly influences the composite properties. However, it is hard to improve impregnation and the fiber volume fraction in fiber‐reinforced thermoplastics because thermoplastic resins have high melt viscosities. This study explored a reformative solution impregnation method for molding fabric‐reinforced thermoplastic composites with a high fiber volume fraction. The fiber volume fraction was significantly increased, to 60%, which is equal to that of fiber‐reinforced thermosetting plastic materials. A comparison indicated that a fiber‐reinforced thermoplastic and a fiber‐reinforced thermosetting plastic with the same reinforcing fiber had similar tensile properties and that the proposed molding method is effective in thermoplastic composite manufacture. POLYM. COMPOS., 34:953–958, 2013. © 2013 Society of Plastics Engineers     

Reactivity Analysis of New Multivalent Electrophilic Probes for Affinity Labeling of Carbohydrate Binding Proteins

We have designed and synthesized six different multivalent electrophiles as carbohydrate affinity labeling probes. Evaluation of the reactivity of the electrophiles against peanut agglutinin (PNA) and Ricinus communis agglutinin (RCA) showed that p- and m-aryl sulfonyl fluoride are effective protein reactive groups that label carbohydrate binding lectins in a ligand-dependent fashion at a nanomolar probe concentration. Analysis of the selectivity of affinity labeling in the presence of excess BSA as a nonspecific protein indicated that m-arylsulfonyl fluoride is a more selective protein-reactive group, albeit with attenuated reactivity. Further analysis showed that the labeling efficiency of the multivalent electrophilic probes can be improved by employing reaction conditions involving 25 °C instead of typically employed 4 °C. Both isomers of arylsulfonyl fluoride groups together represent promising affinity labels for target identification studies that could serve as more efficient alternatives to photoreactive groups.     

Fundamental evaluation of direct-cooling technology for in-wheel drive system

We are developing a small and lightweight direct-drive system toward the realization of in-wheel electric vehicles (EVs). To increase the power density of the motor, we developed a direct-cooling motor that immersed the core, coils, and magnets in cooling oil and improved cooling efficiency. Moreover, we performed measurements of frictional loss, measurement of pressure drop, and a continuous heat-run test on the test bench. The results demonstrated that direct cooling is effective and continuous operation can be achieved. Additionally, it was found that the coil temperature can be controlled by the flow rate of the cooling oil. In this paper, we report on the concept of direct cooling and the measurement results.     

Simultaneous brightness contrast with three different stimuli: 2D, 3D,and D-up

In this study, simultaneous brightness contrast was investigated using three different devices: 2D (paper), 3D (space), and D-up. The paper stimuli were made by printing papers, the space stimuli were produced by illumination in a two-rooms experimental booth, and the D-up stimuli were shown to subjects using a D-up viewer. The brightness of test patches was judged by the amounts of whiteness and blackness. The results were different among the devices; however, if the whiteness of the test patches was plotted for the luminance contrast of the test patch versus the surround, all the data points were expressed by nonlinear equations. Data from the space stimuli were expressed by only one equation; those from the paper stimuli were also expressed by the nonlinear equation but with different coefficients for each test patch. The results from D-up were between those of the space and paper experiments. All the results were explained in relation to the hypothesized illuminated space over the surrounds. The difference among devices was explained by the whiteness of the surround being transferred to the illumination.