Anti-biofilm Activity of Human Milk Oligosaccharides in Clinical Strains of Streptococcus agalactiae with Diverse Capsular and Sequence Types

Group B Streptococcus (GBS) is an encapsulated Gram-positive bacterial pathogen that causes severe perinatal infections. Human milk oligosaccharides (HMOs) are short-chain sugars that have recently been shown to possess antimicrobial and anti-biofilm activity against a variety of bacterial pathogens, including GBS. We have expanded these studies to demonstrate that HMOs can inhibit and dismantle biofilm in both invasive and colonizing strains of GBS. A cohort of 30 diverse strains of GBS were analyzed for susceptibility to HMO-dependent biofilm inhibition or destruction. HMOs were significantly effective at inhibiting biofilm in capsular-type- and sequence-type-specific fashion, with significant efficacy in CpsIb, CpsII, CpsIII, CpsV, and CpsVI strains as well as ST-1, ST-12, ST-19, and ST-23 strains. Interestingly, CpsIa as well as ST-7 and ST-17 were not susceptible to the anti-biofilm activity of HMOs, underscoring the strain-specific effects of these important antimicrobial molecules against the perinatal pathogen Streptococcus agalactiae.     

The Innate Immune Glycoprotein Lactoferrin Represses the Helicobacter pylori cag Type IV Secretion System

Chronic infection with Helicobacter pylori increases risk of gastric diseases including gastric cancer. Despite development of a robust immune response, H. pylori persists in the gastric niche. Progression of gastric inflammation to serious disease outcomes is associated with infection with H. pylori strains which encode the cag Type IV Secretion System (cag T4SS). The cag T4SS is responsible for translocating the oncogenic protein CagA into host cells and inducing pro-inflammatory and carcinogenic signaling cascades. Our previous work demonstrated that nutrient iron modulates the activity of the T4SS and biogenesis of T4SS pili. In response to H. pylori infection, the host produces a variety of antimicrobial molecules, including the iron-binding glycoprotein, lactoferrin. Our work shows that apo-lactoferrin exerts antimicrobial activity against H. pylori under iron-limited conditions, while holo-lactoferrin enhances bacterial growth. Culturing H. pylori in the presence of holo-lactoferrin prior to co-culture with gastric epithelial cells, results in repression of the cag T4SS activity. Concomitantly, a decrease in biogenesis of cag T4SS pili at the host-pathogen interface was observed under these culture conditions by high-resolution electron microscopy analyses. Taken together, these results indicate that acquisition of alternate sources of nutrient iron plays a role in regulating the pro-inflammatory activity of a bacterial secretion system and present novel therapeutic targets for the treatment of H. pylori-related disease.     

"Where similarity beats redundancy: The importance of context, higher order similarity, and response assignment": Correction to Eidels, Townsend, and Pomerantz (2008).

Reports an error in "Where similarity beats redundancy: The importance of context, higher order similarity, and response assignment" by Ami Eidels, James T. Townsend and James R. Pomerantz (Journal of Experimental Psychology: Human Perception and Performance, 2008[Dec], Vol 34[6], 1441-1463). The DOIs for the article and the supplemental materials were incorrectly listed. The correct DOIs are provided in the erratum. (The following abstract of the original article appeared in record 2008-16831-008.) People are especially efficient in processing certain visual stimuli such as human faces or good configurations. It has been suggested that topology and geometry play important roles in configural perception. Visual search is one area in which configurality seems to matter. When either of 2 target features leads to a correct response and the sequence includes trials in which either or both targets are present, the result is a redundant-target paradigm. It is common for such experiments to find faster performance with the double target than with either alone, something that is difficult to explain with ordinary serial models. This redundant-targets study uses figures that can be dissimilar in their topology and geometry and manipulates the stimulus set and the stimulus-response assignments. The authors found that the combination of higher order similarity (e.g., topological) among the features in the stimulus set and response assignment can effectively overpower or facilitate the redundant-target effect, depending on the exact nature of the former characteristics. Several reasonable models of redundant-targets performance are falsified. Parallel models with the potential for channel interactions are supported by the data. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A new interpretation of depth-age profiles

Dosimetry and other techniques provide depth-age profiles in materials as diverse as sediments and ice. A frequent characteristic feature of these plots is a non-linear curve with a foreshortened depth scale. Whilst the age analyses from the dosimetry do not present a problem, many authors have made subsequent assumptions on the basis of the curve shape with respect to climate and environmental changes. Such claims should be strongly questioned, as by using a model which includes the effects of compaction, most of the original curves transform into linear plots corresponding to constant depositional rates. The corrections and the implications for models of climate change are discussed.     

Estimating the Hydraulic Conductivity of Landfilled Municipal Solid Waste Using the Borehole Permeameter Test

This paper reports the in situ field saturated hydraulic conductivity of municipal solid waste at a landfill in Florida. The saturated hydraulic conductivity (Ks) was estimated at 23 locations using the borehole permeameter test, a method commonly used for determination of the Ks of unsaturated soil. The Ks of the landfilled waste was found to range from 5.4×10?6 to 6.1×10?5?cm/s. The Ks was found to be on the lower end of the range of Ks reported by previous studies. The hydraulic conductivity of the waste decreased with depth, the likely result of greater overburden pressures associated with deep locations of the landfill. Permeability values (kw) of the landfilled waste calculated based on Ks were compared with permeability values estimated using air as the fluid (air permeability, ka). Values of ka were found to be approximately three orders of magnitude greater than those of kw. The lower permeability of the waste to water was primarily attributed to entrapped gas. Other factors such as potential clogging of media and short-circuiting of air along the well may also have contributed to the differences in ka and kw.     

Performance and design of InGaAs /InP photodiodes for single-photon counting at 1.55 microm

The performance of selected, commercially available InGaAs/InP avalanche photodiodes operating in a photon-counting mode at an incident wavelength of 1.55 mum is described. A discussion on the optimum operating conditions and their relationship to the electric field distribution within the device is presented.     

The crying infant

The crying infant is a common presenting complaint and a difficult diagnostic dilemma that may represent the primary manifestation of a serious or even life-threatening condition. Although many children experience an exacerbation of the normal crying tendencies or minor ailments typical of the early months of life, a significant number of infants have underlying pathologic conditions requiring immediate intervention. This article briefly reviews current and past research on this phenomenon and presents differential diagnoses and recommendations for the evaluation and management of the acute crying episode.     

Foundations of psychological assessment: Implications for cognitive assessment in clinical science.

We examine psychological assessment within the broader framework of psychology's efforts to build and test useful scientific theories. In the first section, we consider in detail a number of fundamental epistemological, conceptual, and methodological issues that tend either to inhibit or to foster theoretical progress in psychology. In light of these issues, we then recommend that psychology adopt an information-based, quantitative approach to theory building and testing. This approach should help us model the dynamic, stochastic processes underlying human behavior. In the second section, we explore the implications of the issues and strategies that we outlined in the first section for the future of clinical assessment, with a particular focus on the clinical assessment of cognitive processes. We conclude by advocating a conceptual and methodological integration of clinical and cognitive neuroscience in psychology. (PsycINFO Database Record (c) 2010 APA, all rights reserved)