Determination of fat in olestra-containing savory snack products by capillary gas chromatography

A quantitative method to determine fat in olestra-containing savory snack products was validated within the AOAC Peer-Verified Methods Program. The method may be used to demonstrate compliance with the guidelines of the U.S. Nutrition Labeling and Education Act for labeling products as "fat free" or "low fat." The method can measure total and saturated fat in savory snacks when present at levels of 0.2-10 g total fat and 0.1-3 g saturated fat per 30 g serving. The method is standardized to measure C6-C24 fatty acids. Extraction of olestra-containing savory snack samples with chloroform-methanol (modified AOAC Official Method 983.23) yields a lipid extract containing the total fat and olestra. The extracted lipid is hydrolyzed by lipase, yielding fatty acids and unreacted olestra. The fatty acids are precipitated as calcium soaps. Olestra is extracted from insoluble soaps with hexane and then discarded. The isolated soaps are converted back into fatty acids with hydrochloric acid and extracted with hexane. The isolated fatty acids are converted to methyl esters with boron trifluoride-methanol and quantitated by capillary gas chromatography using internal standard. Test samples were prepared by blending olestra-containing and full-fat (triglyceride) snacks to obtain 6 levels of spiking (0-10 g total fat added/30 g serving) in potato chips, potato crisps, cheese puffs, and nacho cheese-flavored corn chips. Results were linear (r2 > 0.997) between 0 and 10 g fat/30 g serving for each product matrix. Mean recovery was 101 +/- 6% standard deviation (SD) for total fat and 104 +/- 6% SD for saturated fat. Mean recovery by peer laboratory was 88 +/- 5% SD for total fat and 95 +/- 4% SD for saturated fat in potato chips (0-3 g total fat added/30 g serving). Two sets of 10 replicates of potato chips (0.5 g total fat/30 g serving and 0.16 g saturated fat/30 g serving) and potato crisps (0.5 g total fat/30 g serving and 0.16 g saturated fat/30 g serving) were analyzed by submitting and peer laboratories. Repeatability relative standard deviations ranged from 3.90 to 7.33% for total fat and from 4.01 to 11.53% for saturated fat. Reproducibility relative standard deviations were 7.33% (total fat, potato chips), 7.15% (total fat, potato crisps), 11.36% (saturated fat, potato chips), and 13.50% (saturated fat, potato crisps).     

The first measurement of an absolute surface concentration of reaction intermediates in ethylene hydrogenation

The first measurement of a turnover rate with respect to surface intermediate concentration in a high pressure heterogeneous catalytic reaction is reported. By using infrared-visible sum frequency generation to study the hydrogenation of ethylene on Pt(111), it was found that the surface concentration of -bonded ethylene, the key reaction intermediate, represented approximately 4% of a monolayer. Thus the absolute turnover rate per surface adsorbed ethylene molecule is 25 times faster than the rate measured per platinum atom. To explain these results, we propose a model of weakly adsorbed ethylene intermediates reacting on atop sites.     

Design rules for an integratable low-power amplifier/filter combination

In this paper design rules for a circuit topology in which there is an inseparable combination of an amplifier and a filter characteristic, are presented. By intentionally using the capacitance of an already present input sensor for the filtering, the total required integrated capacitance is much less than that in circuits, which have a separately designed amplifier and filter function. Consequently, it is possible to have the advantage of a better integratability. Moreover, less complexity in the design is achieved. The presented circuit shows a current-to-voltage conversion and an inherently controllable second-order low-pass filter characteristic. A discrete realization has been designed to test the circuit. This circuit operates down to a 1 V supply voltage and the transfer shows a 1.8 M currentto-voltage conversion with a bandwidth of 6 kHz. Measurement results of this circuit show that a 63 dB dynamic range can be achieved with a total required integrated capacitance of only 31 pF.     

A clinical evaluation in children of the Pharmacia ImmunoCAP system for inhalant allergens

BACKGROUND: The Pharmacia ImmunoCAP system (CAP) for assaying serum IgE specific antibodies was evaluated in a clinical setting against skin-prick test (SPT) performed using Dome/Hollister-Steir allergen extracts. The five common inhalant allergens D. pteronyssinus, D. farinae, mould mix, grass mix and cat epithelium were tested concurrently by both methods in 167 children aged 7.5-12 years. The specific SPT for D. pteronyssinus and D. farinae were also tested against the CAP house dust mite (HDM) mix. OBJECTIVE: The purpose of the study was to determine the sensitivity and specificity of the Pharmacia ImmunoCAP system for detecting serum IgE specific antibodies to inhalant allergens in a clinical setting, using SPT result as the "gold standard'. METHODS: The SPTs were performed using Dome/Hollister-Steir allergen extracts. The serum IgE specific antibodies were quantitated using the radioimmunoassay version of the Pharmacia ImmunoCAP system. A history of allergic disease was assessed using a validated questionnaire. RESULTS: SPT gave more positive reactions than CAP with the exception of cat epithelium. The concordance between SPT and CAP results was 91% over all the tests. The concordance with SPT was slightly higher for the specific CAP for D. pteronyssinus and D. farinae (93% and 95% respectively) than for the CAP HDM mix (86% and 90% respectively). There was a higher proportion of positive results for both SPT and CAP in the 115 children defined as having a history of allergic disease. Using SPT defined allergy as the gold standard, the sensitivity of the CAP system was 87% for the two specific house dust mites but was lower for cat epithelium (67%), mould mix (59%) and grass mix (46%). The sensitivity of the CAP system improved for D. pteronyssinus (96%) and the HDM mix (91%) when tested in subjects defined as having a history of allergy associated disease. The specificity of the CAP system showed less variation between allergens and ranged from 90-99%. CONCLUSION: The results of this study of children aged 7.5-12 years demonstrate that, for the inhalant allergens tested, the Pharmacia ImmunoCAP system performs well in the setting of known allergic disease.     

监控更广的网络领域

Ron Martin说:新技术是现代工业网络中的信息技术(IT)和操作技术(OT)的界限越来越模糊。因此,各方组织机构正在寻找能够监控他们以太网网络的新方法。     

Optimization of a proton exchange membrane fuel cell membrane electrode assembly

A computational framework for fuel cell analysis and optimization is presented as an innovative alternative to the time consuming trial-and-error process currently used for fuel cell design. The framework is based on a two-dimensional through-the-channel isothermal, isobaric and single phase membrane electrode assembly (MEA) model. The model input parameters are the manufacturing parameters used to build the MEA: platinum loading, platinum to carbon ratio, electrolyte content and gas diffusion layer porosity. The governing equations of the fuel cell model are solved using Netwon’s algorithm and an adaptive finite element method in order to achieve near quadratic convergence and a mesh independent solution respectively. The analysis module is used to solve the optimization problem of finding the optimal MEA composition for maximizing performance. To solve the optimization problem a gradient-based optimization algorithm is used in conjunction with analytical sensitivities. By using a gradient-based method and analytical sensitivities, the framework presented is capable of solving a complete MEA optimization problem with state-of-the-art electrode models in approximately 30 min, making it a viable alternative for solving large-scale fuel cell problems.     

命名和征服(英文)

This paper introduces a technique that has been used successfully in University College Dublin,Ireland and Fudan University in Shanghai,China,to teach the formal aspects of computer programming to undergraduate students.The technique,name and conquer,relies on the use of modelling small mathematical domains and allowing the results of these models to form the basis for the programming task.     

Matching with don't-cares and a small number of mismatches

In matching with don't-cares and k mismatches we are given a pattern of length m and a text of length n, both of which may contain don't-cares (a symbol that matches all symbols), and the goal is to find all locations in the text that match the pattern with at most k mismatches, where k is a parameter. We present new algorithms that solve this problem using a combination of convolutions and a dynamic programming procedure. We give randomized and deterministic solutions that run in time O(nk²logm) and O(nk³logm), respectively, and are faster than the most efficient extant methods for small values of k. Our deterministic algorithm is the first to obtain an O(polylog(k)⋅nlogm) running time.     

Utility function of TCP

Understanding the TCP congestion control mechanism from a global optimization point of view is not only important in its own right, but also crucial to the design of other transport layer traffic control protocols with provable properties. In this paper, we derive a global utility function and the corresponding optimal control law, known as TCP control law, which maximizes the global utility. The TCP control law captures the essential behaviors of TCP, including slow start, congestion avoidance, and the binary nature of congestion feedback in TCP. We find that the utility function of TCP is linear in the slow start phase and is proportional to the additive increase rate and approaches the well-known logarithm function as the data rate becomes large in the congestion avoidance phase. We also find that understanding the slow start phase with a fixed threshold is critical to the design of new transport layer control protocols to enable quality of service features. Finally, as an application, we design a Minimum Rate Guaranteed (MRG) traffic control law that shares the same utility function as the TCP control law. Our simulation study of the MRG control law indicates that it is indeed TCP friendly and can provide minimum rate guarantee as long as the percentage of network resource consumed by the MRG flows is moderately small.     

Immunity-based hybrid evolutionary algorithm for multi-objective optimization in global container repositioning

The development of evolutionary algorithms for optimization has always been a stimulating and growing research area with an increasing demand in using them to solve complex industrial optimization problems. A novel immunity-based hybrid evolutionary algorithm known as Hybrid Artificial Immune Systems (HAIS) for solving both unconstrained and constrained multi-objective optimization problems is developed in this research. The algorithm adopts the clonal selection and immune suppression theories, with a sorting scheme featuring uniform crossover, multi-point mutation, non-dominance and crowding distance sorting to attain the Pareto optimal front in an efficient manner. The proposed algorithm was verified with nine benchmarking functions on its global optimal search ability as well as compared with four optimization algorithms to assess its diversity and spread. Sensitivity analysis was also carried out to investigate the selection of key parameters of the algorithm. It is found that the developed immunity-based hybrid evolutionary algorithm provides a useful means for solving optimization problems and has successfully applied to the problem of global repositioning of containers, which is one of a constrained multi-objective optimization problem. The developed HAIS will assist shipping liners on timely decision making and planning of container repositioning operations in global container transportation business in an optimized and cost effective manner.