Deletion and insertion of a 192-residue peptide in the active-site domain of glycosyl hydrolase family-2 beta-galactosidases

The monomeric multimetal-binding beta-galactosidase of Saccharopolyspora rectivirgula (srbg), a glycosyl hydrolase family-2 enzyme, has a unique sequence consisting of 192 amino acid residues with no similarity to known proteins. This 192-residue sequence (termed the "iota [iota] sequence") appears to be inserted into a sequence homologous to the active-site domain of the Escherichia coli lacZ enzyme (lacZbg). To assess the effects of the t sequence at specific sites of beta-galactosidase on the catalytic functioning and molecular properties of beta-galactosidase, deletion or insertion mutants of beta-galactosidases were constructed, expressed in LacZ- E. coli strains, and characterized: srbgdelta in which the iota sequence was deleted from srbg, and lacZbgI, in which the 192-residue iota sequence was inserted into the corresponding position (between Asp591 and Phe592) in the active-site domain of lacZbg. srbgdelta was a catalytically inactive, dimeric protein which retained multimetal-binding characteristics, suggesting that the iota sequence is very important for maintaining the structure necessary for the catalytic functioning and the monomeric structure of srbg but is not responsible for the unique metal ion requirements of srbg. On the other hand, lacZbgI existed as a mixture of a monomer, a tetramer, and higher multimers. The monomeric species was inactive, whereas the tetramer and other multimers were catalytically active (V(max )K(m) value, 25% of that of lacZbg) and highly specific for beta-D-galactoside. The tetrameric lacZbgI was activated by Mg2+ and Mn2+ with lowered metal affinities, and the stoichiometry of metal binding was unchanged from that of lacZbg. These results, along with the published stereo structure of lacZbg, suggest that, in lacZbgI, the inserted 192-residue iota peptide could fold independently of the lacZbg domains into a "sub-domain," lying distant from the active site and subunit interfaces.     

A novel gene delivery system in plants with calcium alginate micro-beads

We have produced micrometer-sized calcium alginate beads referred to as "bio-beads" that encapsulate plasmid DNA molecules carrying a reporter gene. In order to evaluate the efficiency of the bio-beads in mediating genetic transfection, protoplasts isolated from cultured tobacco cells (BY-2) were transfected with bio-beads containing a plasmid that carries the modified green fluorescent protein gene CaMV35S-sGFP. With the bio-beads treatment, approximately ten-fold higher GFP expression was observed after 24 h incubation compared to that with the conventional method using a naked plasmid solution. Transfection was up to 0.22% efficient. These results indicate that bio-beads have a possibility for efficient transformation in plants.     

Novel sugar phosphotransferase system applicable to the efficient labeling of the compounds synthesized via the non-mevalonate pathway in Escherichia coli

It is known that 1-deoxy-D-xylulose taken up by Escherichia coli is used as the precursor of the compounds synthesized via the non-mevalonate pathway, such as isoprenoids, probably after conversion into 1-deoxy-D-xylulose 5-phosphate. In this report, we show that a novel phospho(enol) pyruvate-dependent phosphotransferase system catalyzes the uptake and phosphorylation of 1-deoxy-D-xylulose.     

Implementation of Fault-Tolerant GridRPC Applications

A task parallel application is implemented with Ninf-G, a GridRPC system. A series of experiments are conducted on the Grid testbed in Asia Pacific for three months. Through tens of long executions, typical fault patterns were collected, and instability of the network throughput was determined to be a major reason of the faults. Several important points are stressed to avoid task throughput decline due to the fault-recovery operations: Timeout minimization for fault detection, background recovery, redundant task assignments, and so on. This study also issues a steer for design of the automated fault-tolerant mechanism in an upper layer of the GridRPC framework.     

Quantitative evaluation of display characteristics of AMOLED displays

Abstract— Understanding the display characteristics of OLEDs is not only of general interest but also of technological importance for expanding the application of OLEDs. The display characteristics of AMOLEDs were quantitatively evaluated and compared with LCD or CRT performance. The fast response time and high contrast ratio, which are attractive characteristics of OLEDs, were also retained under low temperature and bright ambient, respectively. Moreover the luminance and color barely changed with viewing angle at any gray‐scale level. The optical design of OLED diodes is important for the emission characteristics, luminance, and color reproducibility.     

Buckling Analysis of TF Coil Inner Leg for Central Solenoidless Tokamak

The central post is one of the critical components for the low aspect ratio tokamak, which endures not only a tremendous ohmic heating because it carries a rather high current, but also a large neutron heating and irradiation owing to the plasma operation. The DS copper alloy Glidcop AL-25[8] was chosen as the conductor material for its adequate mechanical properties and physics properties. The central post has a cylindrical structure with lots of cooling channels. The length of the central post for the next generation of nuclear fusion spherical tokamaks will be more than 10 m or 20 m. The structural stability is very crucial. When the applied load is larger than the structure critical buckling load, the device will lose its stability and collapse. In order to calculate the critical buckling load, a 1/6-segment finite element model was used and the force acting on the central post was simulated. The results showed that the vertical compressive stresses mainly affect the stability of the central post. The linear buckling analysis results with finite element method based on small deformation theory were given in this paper. The relation curves and functions for buckling factor, depending on the different lengths and the radius of the central post, the diameter of cooling channel and the maximum allowable current density, were also shown.     

Multilingual disaster information system: information delivery using graphic text for mobile phones

A multilingual disaster information system (MLDI) has been developed to overcome the language barrier during times of natural disaster. MLDI is a web-based system that includes templates in nine languages so that translated texts can be made available immediately. Mobile phone e-mail with graphic text is a useful tool for delivering multilingual disaster information. The visibility of graphic text on mobile phones was measured and found to be equivalent to the built-in font. However, visibility deteriorates as the character size becomes smaller, especially, on displays with poor resolution. This article also discusses the necessity of multilingual information and measures for a safe and barrier-free society.     

Flexible microelectronics: New application for thin‐film transistors in fingerprint sensors

Abstract— Thin‐film transistors (TFTs) are field‐effect transistors that can be used to create large‐scale‐integrated (LSI) circuits. The combination of high‐performance TFTs and transfer technology of the TFTs has the potential to foster the rise of a new flexible microelectronics industry. This paper discusses the current status of flexible microelectronics, using a TFT fingerprint sensor (FPS) as an example. Technology used in active‐matrix displays can easily be applied to the TFT FPS. TFT technology should not be confined to the display industry; its use should be expanded into the semiconductor industry. With the result presented in this paper, we declare a new era of flexible microelectronics open.     

The NMR sensitivity achievable with a slotted-tube resonator

The signal-to-noise ratio achievable with the slotted-tube resonator (STR), a fundamental type of high radiofrequency coil for nuclear magnetic resonance (NMR) experiments, was formulated in an equation. This equation is based on formulae presented by Hoult and Richards [J Magn Reson 24, 71 (1976)] and Hoult and Lauterbur [J Magn Reson 34, 425 (1979)]. The equation assumes that the sample is positioned within the homogeneous region of magnetic fluxB ₁ generated by the STR, and involves no unknown factors. The NMR sensitivity of an experiment with an STR can therefore be predicted, and the equation is applicable to any nuclear species, static field strength, and dimensions of the sample and STR.