Layer-by-layer self-assembled mutilayer films of gold nanoparticles for surface-assisted laser desorption/ionization mass spectrometry

Layer-by-layer (LBL) self-assembled multilayer films of gold nanoparticles (AuNPs) on a silicon wafer were demonstrated to be promising substrates for surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS) of peptides and environmental pollutants for the first time. LBL multilayer films, (AuNPs/PAHC)n, consisting of alternating layers of ammonium citrate capped AuNPs and poly(allylamine hydrochloride) (PAHC) were prepared on a silicon surface. Silicon plates with aggregated AuNPs were more suitable than those with dispersed AuNPs for the SALDI-MS of peptides. The number of particle layers had a significant effect on the laser desorption/ionization of angiotensin I; the peak intensity of the peptide (molecular ion amount) increased with an increase in the number of layers of AuNPs. As a result, the (AuNPs/PAHC)5 multilayer films increased the sensitivity of the angiotensin I to subfemtomoles and raised the useful analyte mass range, thus making it possible to detect small proteins (a 12 kDa cytochrome c). The signal enhancement when using (AuNPs/PAHC)5 may be due to (i) the high absorption of the UV laser light at 337 nm by the AuNP layers, (ii) the low thermal conductivity due to the AuNPs being covered with a thin monolayer of PAHC, and (iii) the increase in the surface roughness (approximately 100 nm) with the number of AuNP layers. Thus, laser-induced rapid high heating of AuNPs for effective desorption/ionization of peptides is possible. In addition, it was found that (AuNPs/PAHC)5 could be used to extract environmental pollutants (pyrene and dimethyldistearylammonium chloride) from very dilute aqueous solutions with concentrations less than 10(-10) mg/mL, and the analytes trapped in the LBL film could be identified by introducing the film directly into the SALDI mass spectrometer without needing to elute the analytes out of the film.     

下一代光交换网络的智能控制平面技术

For the next generation optical switching networks, the intelligent control plane is a key enabling technique. In this paper, we review our recent research activities related to Generalized Multi-Protocol Label Switching ( GMPLS ) and OpenFlow-based control planes for both Wavelength Switched Optical Networks (WSON) and Elastic Optical Networks (EON).     

Mixing mechanism of three‐tip kneading block in twin screw extruders

In recent years, twin screw extruders have been applied to various kinds of polymer processing. It has been important to find their optimum geometrical configurations and operational processing conditions for the best performance of extrusions and products. Many engineers have been evolving numerical and the experimental methods to characterize the mixing performance for twin screw extruders. We have carried out three‐dimensional flow simulations of kneading blocks in intermeshing co‐rotating twin screw extruders by using the finite element method to quantify their ability in distributive and dispersive mixing. We discuss their performance in distributive mixing for three different type of kneading blocks in terms of the residence time distribution and the nearest distance between markers at various periods of time, by using the marker tracking method. Those numerical techniques and applications of mixing indices have enabled us to quantify and evaluate their abilities in distributive mixing of kneading blocks in twin screw extruders.     

Oxidation of Low-Oxygen Silicon Carbide Fibers (Hi-Nicalon) in Carbon Dioxide

Polycarbosilane-derived low-oxygen SiC fibers, Hi-Nicalon, were heat-treated for 36 ks at temperatures from 1273 to 1773 K in CO₂ gas. The oxidation of the fibers was investigated through the examination of mass change, crystal phase, resistivity, morphology, and tensile strength. The mass gain, growth of β-SiC crystallites, reduction of resistivity of the fiber core, and formation of protective SiO₂ film were observed for the fibers after heat treatment in CO₂ gas. SiO₂ film crystallized into cristobalite above 1573 K. Despite the low oxygen potential of CO₂ gas ( p _O2= 1.22 Pa at 1273 K − 1.78 × 10² Pa at 1773 K), Hi-Nicalon fibers were passively oxidized at a high rate. There was a large loss of tensile strength in the as-oxidized state at higher temperatures because of imperfections in the SiO₂ film. On the other hand, the fiber cores showed better strength retention even after oxidation at 1773 K.     

Generative adversarial network for road damage detection

Machine learning can produce promising results when sufficient training data are available; however, infrastructure inspections typically do not provide sufficient training data for road damage. Given the differences in the environment, the type of road damage and the degree of its progress can vary from structure to structure. The use of generative models, such as a generative adversarial network (GAN) or a variational autoencoder, makes it possible to generate a pseudoimage that cannot be distinguished from a real one. Combining a progressive growing GAN along with Poisson blending artificially generates road damage images that can be used as new training data to improve the accuracy of road damage detection. The addition of a synthesized road damage image to the training data improves the F‐measure by 5% and 2% when the number of original images is small and relatively large, respectively. All of the results and the new Road Damage Dataset 2019 are publicly available ( https://github.com/sekilab/RoadDamageDetector ).     

Periodic Accumulation of Voids Observed in Calcium Phosphate Glass-Ceramics

After the crystallization of calcium phosphate glass, a pattern of stripes like the rings of a tree is observed in the glass-ceramic. The pattern of stripes, formed only from a particular glass composition and under specific crystallization conditions, is made up of voids resulting from a difference in density between the original glass and the resulting glass-ceramic. The size and distribution pattern of these voids must be controlled to achieve maximum mechanical properties.     

Recent plant experience and research into fireside corrosion in Japan

The increasing use of heavy oil as a fuel in power stations has given rise to intensified research in the field of fireside corrosion in Japan. The problems which have to be tackled are described in terms of experiences gained in the operation of oil and coal fired boilers. The main types of damage encountered are nonuniform wastage of tubes in connection with carburization. In this context significant interdependences have been found in connection with coal deposition (especially sodium and sulphur contents). On the basis of this experiences and of the results of corrosion tests in the laboratory and in the field it has been found that the measure offering the best prospect for corrosion control might be the use of coextruded tubing which would allow a combination of high corrosion resistance and high creep strength. In addition to that some modified alloys (e.g. Alloy 800 H, CR 30 A or NF709) offer an interesting potential whereby the high chromium content (more than 20%) is the main criterium.     

Relationship of the structure of ureteral muscularis to in vitro spontaneous contractions

We investigated the anatomical structure of the smooth muscle layers of the mid portion of the canine ureter. In transverse sections the inner muscle bundles appear to be oriented longitudinally and the outer muscle bundles appear to be oriented in a circular configuration. In sections cut parallel with the longitudinal axis of the ureter, the inner and outer bundles appear to be oblique and are oriented in a mesh-line manner. In oblique sections, cut parallel with the helically oriented ureteral muscle, bundles appear to be oriented in a lengthwise configuration. These anatomical studies show that canine ureteral smooth muscle is composed of spiral fibers in a mesh structure and that helical muscle strips which include a significant percentage of continuous bundles have a greater capacity to generate spontaneous contractions in vitro than do transverse and longitudinal muscle strips.