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Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) GS28 and syntaxin 5 can be reciprocally coimmunoprecipitated from Golgi extracts, suggesting that they exist in a protein complex. When Golgi extract is preincubated with soluble NSF attachment proteins (alpha-SNAP) and N-ethylmaleimide-sensitive factor (NSF) under conditions that allow ATP hydrolysis by NSF, GS28 and syntaxin 5 become dissociated. GS28 and syntaxin 5 remain in a protein complex when Golgi extract is preincubated with similar amounts of alpha-SNAP and NSF under conditions that prevent ATP hydrolysis by NSF, suggesting that ATP hydrolysis by NSF is necessary for dissociating the GS28-syntaxin 5 complex. Since preincubation of Golgi extract with either alpha-SNAP or NSF alone has no effect on the GS28-syntaxin 5 complex, a concerted action of alpha-SNAP and NSF therefore mediates the dissociation of the GS28-syntaxin 5 complex. Furthermore, GS28 but not syntaxin 5 is capable of binding to immobilized alpha-SNAP when the GS28-syntaxin 5 complex is dissociated. 相似文献
73.
A method of determining the optimum beam intensities for compensation using multiple static multileaf collimator fields is presented. In this method a histogram of the number of beam pixels against beam intensity is generated for the intensity-modulated beam (IMB). The intensity of each beam to be used is chosen to minimize the mean square deviation between each bin in the histogram and the closest beam intensity. This method has been applied to sample IMBs possessing one maximum and two maxima. For both cases, the use of uniform beam intensity increments is shown to be close to optimal. In the case with two maxima, the efficacy of irradiating both peaks simultaneously, rather than separately, has been studied and shown to be of potential benefit. The optimum intensities for an IMB for breast radiotherapy are also presented. 相似文献
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VN Titov 《Canadian Metallurgical Quarterly》1997,43(4):195-207
System of lipoproteins serves for the transport of fatty acids in the blood. Triacylglycerols are the transport form of saturated (mono-unsaturated and trans-forms) fatty acids forming crystal phase. Phospolipids and cholesterol ethers are transport forms of polyunsaturated acids in the polar and crystal phases, respectively. Even in the forms of complex lipids saturated and polyunsaturated fatty acids are transported in the blood by various apoproteins. ApoB-48 transports saturated fatty acids to hepatocytes in the form of chilomicrons. ApoA-1 transports transports polyunsaturated fatty acids directly to cells including hepatocytes. ApoA-100 contains two lipid binding domains. It transports saturated fatty acids and their cholesterol ethers associated with the third and fifth domains, respectively. For the structural function polyunsaturated fatty acids are transported by ApoA-1 high density lipoproteins in the polar phase and they penetrate into cells via phospholipid re-esterification. 相似文献
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Complete clinical and physiologic examination of 415 tunnelers exposed to intensive vibration in cooling microclimate proved that extreme functioning of thermoregulation results in shorter development, quicker progression of vibration disease and abortive types of the disease. The data obtained were helpful to base treatments and prophylaxis including special clothes, appropriate therapy. 相似文献
79.
The role of a marker mutation and other genes in a decrease in viability was studied in the Drosophila melanogaster vg line. In flies of the C-S line, chromosome 2 was substituted by the homologous chromosome of the vg flies. In addition, the flies of the mutant phenotype with mutant genes partially or completely substituted by the wild-type C-S genes were obtained in saturating crosses C-S x vg. In the reciprocal variant of chromosome 2 substitution, the flies of the C-S phenotype with chromosomes 1, 3, and 4 from the vg line were obtained. Chromosome 2 of the vg line, introduced into C-S fly karyotype, proved to substantially reduce the heat resistance and life span of flies. In the case of reciprocal replacement (C-S line chromosome 2 substituted for the homologous chromosome of vg flies), a significant increase in viability was observed, which, however, never reached the level characteristic of the C-S line. As the vg genotype became saturated with C-S genes, the heat resistance and life span of flies increased substantially. However, even the complete saturation of mutant chromosomes with wild-type genes never resulted in the equal viability of vg and C-S flies. These data suggest that the low viability of the vg mutant is largely accounted for by the gene composition of the second chromosome and, primarily, by the presence of the vg gene. Nevertheless, there is evidence that, along with the pleiotropic effect of the marker mutation, other genes not linked to chromosome 2 are responsible for the studied physiological properties of the vg flies. 相似文献
80.