We report the implementation of an electrostatic Einzel lens (Boersch) phase plate in a prototype transmission electron microscope dedicated to aberration-corrected cryo-EM. The combination of phase plate, Cs corrector and Diffraction Magnification Unit (DMU) as a new electron-optical element ensures minimal information loss due to obstruction by the phase plate and enables in-focus phase contrast imaging of large macromolecular assemblies. As no defocussing is necessary and the spherical aberration is corrected, maximal, non-oscillating phase contrast transfer can be achieved up to the information limit of the instrument. A microchip produced by a scalable micro-fabrication process has 10 phase plates, which are positioned in a conjugate, magnified diffraction plane generated by the DMU. Phase plates remained fully functional for weeks or months. The large distance between phase plate and the cryo sample permits the use of an effective anti-contaminator, resulting in ice contamination rates of <0.6 nm/h at the specimen. Maximal in-focus phase contrast was obtained by applying voltages between 80 and 700 mV to the phase plate electrode. The phase plate allows for in-focus imaging of biological objects with a signal-to-noise of 5-10 at a resolution of 2-3 nm, as demonstrated for frozen-hydrated virus particles and purple membrane at liquid-nitrogen temperature. 相似文献
In a paper recently published (ibid., vol.12, p. 30-8, 1993), Chiang and Sullivan compare a new criterion for image registration called CBC (coincident bit counting) with two criteria that the authors proposed some years ago, namely SSC and DSC (stochastic and deterministic sign change criteria). The authors' nonparametric approach was demonstrated to outperform the conventional image registration criteria for robust registration in the fact that the value of their similarity measure did not take the specific pixel values into account. In light of this observation, Chiang and Sullivan have built the CBC criterion. The CBC method compares the number of coincident bits between the corresponding pixels in two different frames for a fixed amount of displacement. While the authors consider that the CBC is of interest and deserves to be studied, they feel that the comparison made by Chiang and Sullivan was not entirely accurate. Here the authors comment on this comparison and suggest possible further studies. 相似文献
Discusses the evolution of the Psychological Review from its inception in 1894 as a general journal to its current status as a forum for theoretical discussion. A historical overview of Psychological Review is presented, and its 2 main subfields, cognition and perception, are outlined. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
We introduce a novel algorithm for online estimation of Acoustic Impulse Responses (AIRs) which allows for fast convergence by exploiting prior knowledge about the fundamental structure of AIRs. The proposed method assumes that the variability of AIRs of an acoustic scene is confined to a low-dimensional manifold which is embedded in a high-dimensional space of possible AIR estimates. We discuss various approaches which exploit a training data set of AIRs, e.g., high-accuracy AIR estimates from the acoustic scene, to learn a local affine subspace approximation of the AIR manifold. The model is motivated by the idea of describing the generally nonlinear AIR manifold locally by tangential hyperplanes and its validity is verified for simulated data. Subsequently, we describe how the manifold assumption can be used to enhance online AIR estimates by projecting them onto an adaptively estimated subspace. Motivated by the assumption of manifolds being locally Euclidean, the parameters determining the adaptive subspace are learned from the nearest neighbor AIR training samples to the current AIR estimate. To assess the proximity of training data AIRs to the current AIR estimate, we introduce a probabilistic extension of the Euclidean distance which improves the performance for applications with non-white excitation signals. Furthermore, we describe how model imperfections can be tackled by a soft projection of the AIR estimates. The proposed algorithm exhibits significantly faster convergence properties in comparison to a high-performance state-of-the-art algorithm. Furthermore, we show an improved steady-state performance for speech-excited system identification scenarios suffering from high-level interfering noise and nonunique solutions.
Extrusions of hollow profiles with weld seams were conducted using the magnesium alloy ME21 applying various extrusion ratios. Subsequent analysis of the profiles’ microstructure was performed comparing weld free with weld seam containing material using (polarized) light optical microscopy (LOM). Additionally, the local texture and microstructure in the weld-free material as well as in the weld seam region has been examined with a scanning electron microscope coupled with electron backscatter diffraction technique (SEM-EBSD). The weld-free material and the weld seam are characterized by recrystallized microstructures, whereas few residual cast grains were identified. The local texture distinctively changes from the weld-free material to the weld seam. The texture of the weld-free material is comparable with the typical ME21 sheet texture. In the weld seam area, a pole density is found, which is distributed towards the transverse direction (TD) combined with a split and broadening of the pole density in the extrusion direction (ED). This texture influences the mechanical anisotropy due to the dependence of the activation of basal 〈a〉-slip and \( \{ 10\bar{1}2\} \;\langle 10\bar{1}1\rangle \)-extension twinning on the loading direction in favorably oriented grains. 相似文献
In order to clarify the influence of intrauterine growth restriction on systemic hemodynamics, catecholamine response, and regional distribution of brain energy metabolites per se and during mild hypoxic episodes a study was performed in thirty newborns with a well-characterized state of intrauterine and intra-natal development. Thirty animals were divided into fifteen normal weight piglets (NW) and fifteen intrauterine growth restricted (IUGR) piglets according to their birth weight. Category "NW" covered animals with a birth weight of > 40th percentile; IUGR category covered animals with a birth weight of > 5th and < 10th percentiles. Animals were anesthetized with halothane in 70% nitrous oxide and 30% oxygen and after immobilization artificially ventilated. The acid-base balance and blood gas values at baseline conditions were similar within the different groups investigated and consistent with other data obtained from anesthetized and artificially ventilated newborn piglets. Mild hypoxic hypoxia which was induced by lowering the FiO2 from 0.35 to 0.15 resulted in reduced arterial pO2 (NW: from 115 +/- 37 mmHg to 39 +/- 7 mmHg; IUGR: from 117 +/- 23 mmHg to 39 +/- 3 mmHg; p < 0.05), but arterial pH and pCO2 remained unchanged. Under baseline conditions arterial blood pressure, cardiac output, and myocardial contractility, expressed as dp/dt(max) and plasma catecholamine values were similar in all groups studied. Heart rate was slightly increased in IUGR (p < 0.05). Mild hypoxia led to a strong increase of myocardial contractility in NW as well as IUGR piglets to 2.4 and 2.7 fold and remained increased during recovery (p < 0.05). Moreover, total peripheral resistance was enhanced at the end of recovery period in IUGR animals (p < 0.05). There was a significant increase of epinephrine (E) in NW animals in comparison to sham-operated animals (p < 0.05). Interestingly, during reoxygenation the further increase in E and norepinephrine (NE) levels were enhanced in the animals which suffered from mild hypoxia (p < 0.05). Regional distribution of brain tissue metabolites was partly affected by intrauterine growth restriction. In particular, brain tissue glucose content was strongly reduced by 65 to 72 per cent in all brain regions investigated. Mild hypoxia led to an increase of about 30 percent in NW animals (p < 0.05). In IUGR piglets the percentage increase of brain glucose content was on an average more pronounced but with considerably higher variance. Also, a strong increase of brain lactate content appeared here (p < 0.05). In contrast, brain tissue ATP was quite similar in all groups studied, but brain creatine phosphate was significantly reduced in some forebrain structures of IUGR piglets after mild hypoxia (figure 2, p < 0.05). In summary, this investigation provides information on cardiovascular functions and brain metabolites of normal weight and naturally occurring growth restricted newborn piglets. Mild hypoxemia was well-tolerated from both animal groups. It is suggested that lactate may play a significant role as a source for brain energy production in the newborn IUGR piglets. 相似文献