Colored Fluorescent Silk Made by Transgenic Silkworms

Silk is a protein fiber used to weave fabrics and as a biomaterial in medical applications. Recently, genetically modified silks have been produced from transgenic silkworms. In the present study, transgenic silkworms for the mass production of three colors of fluorescent silks, (green, red, and orange) are generated using a vector originating from the fibroin H chain gene and a classical breeding method. The suitability of the recombinant silks for making fabrics is investigated by harvesting large amounts of the cocoons, obtained from rearing over 20 thousand silkworms. The application of low temperature and a weakly alkaline solution for cooking and reeling enables the production of silk fiber without loss of color. The maximum strain tolerated and Young's modulus of the fluorescent silks are similar to those of ordinary silk, although the maximum stress value of the recombinant silk is slightly lower than that of the control. Fabrics with fluorescent color are demonstrated using the recombinant silk, with the color persisting for over two years. The results indicate that large amounts of genetically modified silk can be made by transgenic silkworms, and the silk is applicable as functional silk fiber for making fabrics and for use in medical applications.     

Characterization of plasma membrane H+-ATPase from salt-tolerant yeast Candida versatilis

Plasma membrane was isolated from the salt-tolerant yeast Candida versatilis and the ATPase in plasma membrane was characterized. The ATPase was a typical H⁺-ATPase with similar properties to the Saccharomyces cerevisiae and Zygosaccharomyces rouxii enzymes. It was reacted with antibody (IgG) raised against S. cerevisiae plasma membrane H⁺-ATPase. The ATPase activity was not changed by adding NaCl and KCl to the assay solutions, but was increased by NH, especially by ammonium sulfate. In vivo stimulation of ATPase activity was observed by the addition of NaCl into the culture medium, as observed in Z. rouxii. No in vivo activation of H⁺-ATPase by glucose metabolism was observed in C. versatilis cells and the activity was independent of the growth phase, like Z. rouxii and unlike S. cerevisiae cells.     

Study on an after-laying test for a long-distance GIL

Technologies for the after-laying test of long-distance SF₆ gas insulated transmission line (GIL) have been developed for the detection of partial discharge (PD) and contact defects. We constructed a long (168 m) test line in a deep tunnel and clarified the propagation characteristics of PD signals through the GIL as well as the spatial sensitivity and S/N characteristics of PD sensors for locating metallic particles. We have also proposed the application of a fiber-optic temperature distribution sensor for locating contact defects and confirmed the validity of this sensor for the GIL test line     

On the basic principles of radar polarimetry: the targetcharacteristic polarization state theory of Kennaugh, Huynen'spolarization fork concept, and its extension to the partially polarizedcase

Basic principles of radar polarimetry are introduced. The target characteristic polarization state theory is developed first for the coherent case using the three stage, the basis transformation, and the power (Mueller) matrix optimization procedures. Kennaugh's and Huynen's theories of radar target polarimetry are verified for the monostatic reciprocal case. It is shown that there exist, in total, five unique pairs of characteristic polarization states for the symmetric scattering matrix of which two pairs, the cross-polarization null and copolarization max pairs, are identical, whereas the cross-pol max and the cross-pol saddlepoint pairs are distinct. The theory is verified by an example for which next to the polarization fork the copolarized and cross-polarized power density plots are also presented. The partially polarized case for completely polarized wave incidence is presented and compared with the results for the coherent and the partially coherent cases, the latter of which is still unresolved     

The new two-dimensional electron gas base HBT (2DEG-HBT):two-dimensional numerical simulation

The bipolar/FET characteristics of the 2DEG-HBT are analyzed extensively by a two-dimensional numerical simulator based on a drift-diffusion model. For bipolar operations at high collector current densities, it is confirmed that the cutoff frequency f_T is determined mainly by the collector transit time of holes and by the charging time of the extrinsic base-collector capacitance C _bc^EXT. The charging times of the emitter and base regions and the base transit time are shown to be negligible. A high cutoff frequency F_T (88 GHz) and current gain h_FE (760) are obtained for an emitter size of 1×10 μm², and undoped collector thickness of 150 nm, and a collector current density J_c of 10⁵ A/cm². The FET operation of the same 2DEG-HBT structure shows a threshold voltage V_th of 0.74 V, the transconductance G_m^max of 80 mS/mm, and maximum cutoff frequency F_T^max of 15 GHz. The dependence of the device performance on material parameters is analyzed extensively from a device design point of view     

Increase in photovoltaic performances of dye-sensitized solar cells—Modification of interface between TiO2 nano-porous layers and F-doped SnO2 layers

In order to improve the physical and chemical contacts between a porous TiO₂ layer and an F-doped SnO₂ transparent conductive layer (FTO), the surface of the FTO layer is polished. After polishing, the surface roughness decreased. However, light transmittance and sheet resistance did not vary largely. The short circuit current (J_sc) and efficiencies increased after the FTO was polished. It was found that the interfacial charge transfer between a TiO₂ layer and an FTO layer decreased by impedance measurement, which suggests that contacts between an FTO and a TiO₂ layer are improved because of the flatted surfaces or removal of electrical impurities. We propose one of the industrially important phenomena that surface polishing of FTO is one of the ways to increase photovoltaic performances for DSCs.     

Development of cube-type SOFC stacks using anode-supported tubular cells

Tubular SOFCs have shown many desirable characteristics such as high thermal stability during rapid heat cycling and large electrode area per unit volume, which can accelerate to realize SOFC systems applicable to portable devices and auxiliary power units for automobile. So far, we have developed anode-supported tubular SOFCs with 0.8–2 mm diameter using Gd-doped CeO₂ (GDC) electrolyte, NiO-GDC anode and (La, Sr)(Co, Fe)O₃ (LSCF)-GDC cathode. In this study, a newly developed cube-type SOFC stack which consists of three SOFC bundles was designed and examined. The bundle consists of three 2 mm diameter tubular SOFCs and a rectangular shaped cathode support where these tubular cells are arranged in parallel. The performance of the stack whose volume is less than 1 cm³ was shown to be 2.8 V OCV and over 1 W at 1.6 V under 500 °C. Cathode loss factor due to current collection from cathode matrix was also estimated using a proposed model.     

Japanese programs on novel concepts in PV

Japanese R&;D activities in photovoltaics (PV) and our R&;D activities with III-V compound multijunction (MJ) solar cells are presented. We have realized high-efficiency InGaP/InGaAs triple-junction solar cells with an efficiency of 36.5–37% (AM1.5G, 200 suns) and concentrator triple-junction solar cell modules with an outdoor efficiency of 27% as a result of designing a grid structure, developing low optical loss Fresnel lens and homogenizers, and designing low thermal conductivity modules. Our challenge now is to develop low-cost and high output power concentrator MJ solar cell modules with an output power of 400 W/m² for terrestrial applications.     

A 120-W Boost Converter Operation Using a High-Voltage GaN-HEMT

A boost converter with a 940-V/4.4 A GaN-HEMT as the main switching device was demonstrated to show the possibility of using high-voltage GaN-HEMTs in power electronic applications. The demonstrated circuit achieved an output power of 122 W and a power efficiency of 94.2% under a drain peak voltage as high as 350 V and a switching frequency of 1 MHz. The dual field-plate structure realized high-voltage switching operation with high power efficiency as dynamic on-resistance was suppressed by an increase of the current collapse phenomena.     

Morphological changes of Candida albicans induced by micafungin (FK463), a water-soluble echinocandin-like lipopeptide

Micafungin (FK463), a novel water-soluble echinocandin-like lipopeptide, exerted fungicidal action toward growing cells of Candida albicans at concentrations of 0.1 microg ml(-1) or above. The drug at these levels induced osmotically fragile cells and the resulting fungicidal effect was partially reversed when cultures were grown in the presence of an osmotic stabilizer. Candida cells incubated with fungicidal concentrations of micafungin gradually increased in size and/or became deformed. An electron microscopic study of such micafungin-treated cells revealed morphological alterations in the cell wall; deformation in contour, abnormal septum formation and decrease in thickness of the intermediate layer of the cell wall were prominent. In addition, the structure of cell membranes as well as of membranous cytoplasmic organelles was slightly impaired. These data suggest that micafungin principally affects the normal formation of the cell wall in growing Candida cells.