Effect of hydrogen on dislocation structures around a mixed-mode fatigue crack tip in a single-crystalline iron–silicon alloy

The effect of hydrogen on dislocation structures around a mixed-mode fatigue crack tip in a single-crystalline iron–silicon alloy was investigated by cross-sectional electron backscatter diffraction and high-voltage electron microscopy. In contrast to the previously reported results in an inert environment, no dislocation cells were formed around the crack tip in a hydrogen environment. The microscopic features around the crack tip suggest that hydrogen promotes a mode I-type crack growth mechanism, which reasonably explains the observed macroscopic crack growth property.     

Modeling and deforming a virtual dense elastic object with the haptic device PHANToM

In recent years, there have been various problems in medical treatments, of which human error by the surgeon in an operation is one of the most serious. In order to minimize human error in an operation, we need a medical training system by which inexperienced surgeons can try operating again and again to improve their skill. In this research, we construct a system of modeling a virtual dense elastic object, and deforming that object using a haptic device called PHANToM. In the system which we construct, we use two PCs to distribute the process of calculation, and SCRAMNet+ is used to connect each PC. PHANToM is used to operate on the object and to express the force which is generated from the deformation of the object. We represent the dense object by using voxels and tetrahedrons, and the elastic object by using a spring-mass model. A virtual dense elastic object is obtained by computed tomography (CT) or magnetic resonance imaging (MRI) to express the patient’s organs.     

Simultaneous determination of aconitine analogues in Aconitum plants and foods that caused food poisoning by liquid chromatography with tandem mass spectrometry

A simple method for the simultaneous determination of four aconitine analogues (AC; aconitine, HA; hypaconitine, MA; mesaconitine, JA; jesaconitine) in Aconitum plants (Aconitum subcuneatum NAKAI) and a food that caused food poisoning was developed, using liquid chromatography tandem mass spectrometry (LC/MS/MS). Aconitine analogues were extracted with 1 mmol/L HCl and then cleaned up with an Oasis HLB cartridge. The LC separation was performed with an octadecylated silica column (Develosil ODS-HG-5, 2.0 mm i.d. x 50 mm) at a flow rate of 0.2 mL/min, using A solution (5 mmol/L ammonium acetate dissolved in 0.1% acetic acid) and B solution (acetonitrile-THF=1 : 3), 90%A (0 min)-->60%A (15 min)-->const. (2 min). Mass spectral acquisition was performed in the positive mode and the analogues were targeted using multiple reaction monitoring (MRM) with electrospray ionization (ESI). The recoveries of aconitine analogues were 93-99% from Aconitum plants. The detection limits of AC, HA, MA and JA were 0.4, 0.4, 0.3 and 0.5 ng/g, respectively. The aconitine analogues, except JA, were detected in food that caused food poisoning at the level of 2.6-29.7 microg/g. These results indicate that the developed method is suitable for the determination of aconitine analogues in Aconitum plants and foods that cause food poisoning.     

Tumor-Derived Small Extracellular Vesicles Involved in Breast Cancer Progression and Drug Resistance

Breast cancer is one of the most serious and terrifying threats to the health of women. Recent studies have demonstrated that interaction among cancer cells themselves and those with other cells, including immune cells, in a tumor microenvironment potentially and intrinsically regulate and determine cancer progression and metastasis. Small extracellular vesicles (sEVs), a type of lipid-bilayer particles derived from cells, with a size of less than 200 nm, are recognized as one form of important mediators in cell-to-cell communication. sEVs can transport a variety of bioactive substances, including proteins, RNAs, and lipids. Accumulating evidence has revealed that sEVs play a crucial role in cancer development and progression, with a significant impact on proliferation, invasion, and metastasis. In addition, sEVs systematically coordinate physiological and pathological processes, such as coagulation, vascular leakage, and stromal cell reprogramming, to bring about premetastatic niche formation and to determine metastatic organ tropism. There are a variety of oncogenic factors in tumor-derived sEVs that mediate cellular communication between local stromal cells and distal microenvironment, both of which are important in cancer progression and metastasis. Tumor-derived sEVs contain substances that are similar to parental tumor cells, and as such, sEVs could be biomarkers in cancer progression and potential therapeutic targets, particularly for predicting and preventing future metastatic development. Here, we review the mechanisms underlying the regulation by tumor-derived sEVs on cancer development and progression, including proliferation, metastasis, drug resistance, and immunosuppression, which coordinately shape the pro-metastatic microenvironment. In addition, we describe the application of sEVs to the development of cancer biomarkers and potential therapeutic modalities and discuss how they can be engineered and translated into clinical practice.     

Direct Inhibition of SARS-CoV-2 Spike Protein by Peracetic Acid

Peracetic acid (PAA) disinfectants are effective against a wide range of pathogenic microorganisms, including bacteria, fungi, and viruses. Several studies have shown the efficacy of PAA against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, its efficacy in SARS-CoV-2 variants and the molecular mechanism of action of PAA against SARS-CoV-2 have not been investigated. SARS-CoV-2 infection depends on the recognition and binding of the cell receptor angiotensin-converting enzyme 2 (ACE2) via the receptor-binding domain (RBD) of the spike protein. Here, we demonstrated that PAA effectively suppressed pseudotyped virus infection in the Wuhan type and variants, including Delta and Omicron. Similarly, PAA reduced the authentic viral load of SARS-CoV-2. Computational analysis suggested that the hydroxyl radicals produced by PAA cleave the disulfide bridges in the RBD. Additionally, the PAA treatment decreased the abundance of the Wuhan- and variant-type spike proteins. Enzyme-linked immunosorbent assay showed direct inhibition of RBD-ACE2 interactions by PAA. In conclusion, the PAA treatment suppressed SARS-CoV-2 infection, which was dependent on the inhibition of the interaction between the spike RBD and ACE2 by inducing spike protein destabilization. Our findings provide evidence of a potent disinfection strategy against SARS-CoV-2.     

Study on rough-surface biaxially oriented polypropylene film. I. Formation of β-form crystals in sheet cast with T-die extruder

Sheets were cast by extruding polypropylenes (PP) which contained γ-quinacridone, a β-crystal nucleator, at levels of 0–10 ppm using a 30 mm ϕ extruder with a T-die at extrusion temperatures of 200–260°C and chill roll temperatures of 30–90°C. The influences of raw resin characteristics such as γ-quinacridone content and MFI of the base PP and casting conditions such as extrusion temperature and chill roll temperature on the amount of β-crystals formed in the sheet were studied. The amount of the β-crystals formed was larger as the γ-quinacridone content was higher and the extrusion temperature was lower and almost independent of the chill roll temperature. As for the influence of MFI of the base PP, the amount of the β-crystals formed was maximum at MFI ≑ 8 dg/min.     

早稻田大学皮秒脉冲辐解装置

A compact pulse radiolysis apparatus using a BNL-type s-band photocathode RF gun is now under development at Waseda University. The laser pulse is used for excitation of the photocathode and for generation of white light, which is used for analyzing light in the pulse radiolysis.The system design is shown in Fig. 1. For absorption spectroscopy with wide wavelength region, the white-light continuum was used as analyzing light. The white light continuum was generated by focusing the high intense IR laser into the water cell. The available wavelength was 400-950 nm. Typical data obtained is shown in Fig.2 by using the system. We have performed a deconvolution technique to know the time resolution of our system. For this purpose, we have assumed the shapes of the electron pulse and the laser pulse as Gaussian distributions. Furthermore, the difference of velocity in a sample cell between the electron pulse and the laser pulse due to the refractive index of a sample was also taken into consideration and assuming that the hydrated electrons are formed as a step function and the response function is Gaussian.Thus, time resolution of the system was estimated by deconvolution of the experimental data.We have achieved a time resolution of about 26ps by measuring time profile of hydrated electrons in H2O.     

Synthesis and characterization of PEFC membranes based on fluorinated-polymer-alloy using pre-soft-EB grafting method

Polymer electrolyte fuel cell (PEFC) membranes based on thin film of crosslinked perfluorinated polymer-alloys (RX-FA) have been fabricated by soft electron beam (soft-EB) grafting with styrene monomers using soft-EB irradiation under nitrogen atmosphere at room temperature (RT). The characteristic properties of styrene-grafted materials (GRX-FA) and sulfonated materials (SRX-FA) have been measured by differential scanning calorimetry (DSC) and FT-IR spectroscopy, ionic conductivity and so on. The glass transition temperatures (dry state) of all obtained SRX-FA were about 105 ± 1 °C, which are higher than Nafion^®. The ion exchange capacities of SRX-FA have been achieved about 3.3 meq/g (dry). The ionic conductivity of obtained SRX-FA has showed about 0.17 S/cm at 60 °C with relative humidity (RH) of ∼95%. The ionic conductivities of the obtained SRX-FA were higher than that of conventional perfluoro-sulfonic acid membranes (PFSA). Fabricated membrane electrode assemblies (MEAs) based on the obtained SRX-FA have shown encouraging performance in the PEFC, compared with the conventional PFSA. The power density of obtained MEAs based on the SRX-FA was about 330-340 mW/cm² under 500 mA/cm² at 60 °C operation. Moreover, the maximum power densities of obtained MEAs based on the SRX-FA shows about 630 mW/cm² at 60 °C. On the other hand, the power density at 500 mA/cm² and maximum power density of MEA based on Nafion^®112 were about 320 and 590 mW/cm² at 60 °C. Thus, the power density of the obtained SRX-FA was higher than that of conventional PFSA.