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91.
Extracted lipids from sesame (Sesamum indicum) seeds of three varieties were determined by high-performance liquid chromatography (HPLC) for endogenous antioxidants. The molecular species and fatty acid (FA) distribution of triacylglycerol (TAG) isolated from total lipids in sesame seeds were analyzed by a combination of argentation thin-layer chromatography (TLC) and gas chromatography (GC), and were investigated in relation to their antioxidant distribution. γ-Tocopherol was present in highest concentration, and δ-, and α-tocopherols were very small amounts. Sesamin and sesamolin were the main lignan components. A modified argentation-TLC procedure, developed to optimize the separation of the complex mixture of total TAG, provided 12 different groups of TAG, based on both the degree of unsaturation and the total acyl-chain length of FA groups. With a few exceptions, the major TAG components were SM2 (6.5–6.7%), SMD (19.8–20.7%), M2D (15.0–26.3%), MD2 (23.6–35.0%), and D3 (7.7–10.7%) (where S denotes a saturated FA, M denotes a monoene, D denotes a diene, and T denotes a triene). It seems that the three varieties were highly related to each other based on the FA composition of the TAG as well as the distribution pattern in the different TAG molecular species. These results suggest that there are no essential differences in the oil components among the three varieties.  相似文献   
92.
Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA‐protein complex. Visualizing the mRNA is crucial for understanding the mechanisms underlying mRNA processing and elucidating its structure and recent advances in mRNA imaging allow detection of real‐time mRNA localization in living cells. However, these techniques revealed only the location of mRNA but cannot visualize the conformation of mRNA‐protein complex in cells. On the other hand, transmission electron microscopy has been used to visualize the structure of the Balbiani ring‐derived large mRNA, but their observations were limited to the insect cells. In this study, we visualized the structure of mRNA‐protein complex in human culture cells by using immuno‐electron microscopy. Through immuno‐detection, an mRNA exon junction binding complex Y14, and its binding protien Upf2, different gold particle patterns were imaged with transmission electron microscopy and analyzed. Characteristic linear and stacked particle orientation were observed. Across the nuclear membrane, only linear aggregation pattern was observed, whereas the stacked aggregation pattern was detected in the cytoplasm. Our method is able to visualize mRNA‐conformation and applicable to many cell types, including mammalian cells, where genes can easily be manipulated.  相似文献   
93.
Volatility of 137 Cs and 106Ru from borosilicate glass containing actual high-level waste was measured in an almost closed stainless-steel canister. The temperature dependence of the volatility of 137Cs was close to that obtained in our previous study using 134Cs. The volatility of 106Ru was about one-fifth that of 137Cs at 600° and 800°C. The air contamination by 137Cs and 106Ru in the canister at 400°C was estimated at 1.8 × 102 and 2 × 10 Bq/cm3, respectively, when it was assumed that the glass contained a realistic amount of 137Cs and 106Ru expected in commercial waste glass. These results are useful for predicting safety in a storage facility under operation.  相似文献   
94.
Summary A facile method to determine the distribution of methyl groups in regioselectively methylated celluloses on anhydroglucose unit was developed using solution 1H-NMR analysis. The determination was deduced from the assignment of the signals of each proton, which directly attached to the glucopyranose ring carbon for partially methylated 2,3-di-O-methylcellulose samples observed in D2O. These data for the distribution of the methyl groups corresponded to those based on the gas-chromatographic analysis. This method using the 1H-NMR spectroscopy can facilitate the measurements of the distribution of methyl groups for randomly substituted and commercially available O-methylcelluloses. Received: 24 August 2000/ Revised version: 10 July 2001/ Accepted: 23 December 2001  相似文献   
95.
Regiospecific distributions of fatty acids of triacylglycerols (TAG) and phospholipids (PL) separated from broad beans (Vicia faba) of four cultivars (Minpo, Sanuki, Nintoku and Sanren) were investigated. The major lipid components were PL (47.5–50.5 wt‐%) and TAG (47.7–50.1 wt‐%), while steryl esters, hydrocarbons, free fatty acids, diacylglycerols and monoacylglycerols were present in minor proportions (1.6–2.4 wt‐%). The PL components isolated from the four cultivars were phosphatidylcholine (56.4–58.4 wt‐%), phosphatidylethanolamine (20.3–21.7 wt‐%) and phosphatidylinositol (16.6–18.6 wt‐%). Phosphatidylinositol was unique in that it had the highest saturated fatty acid content among these PL. The principal characteristics of the fatty acid distribution in the TAG and PL were evident in the beans: Unsaturated fatty acids were predominantly concentrated in the sn‐2 position while saturated fatty acids primarily occupied the sn‐1 or sn‐3 position in these lipids. The lipid components and fatty acid distributions were almost the same in the four cultivars and were not influenced by genetic variability and planting location. These results could be useful information to both consumers and producers for the manufacture of traditional broad bean foods in Japan.  相似文献   
96.
This paper describes BiCMOS level-converter circuits and clock circuits that increase VLSI interface speed to 1 GHz, and their application to a 704 MHz ATM switch LSI. An LSI with a high speed interface requires a BiCMOS multiplexer/demultiplexer (MUX/DEMUX) on the chip to reduce internal operation speed. A MUX/DEMUX with minimum power dissipation and a minimum pattern area can be designed using the proposed converter circuits. The converter circuits, using weakly cross-coupled CMOS inverters and a voltage regulator circuit, can convert signal levels between LCML and positive CMOS at a speed of 500 MHz. Data synchronization in the high speed region is ensured by a new BiCMOS clock circuit consisting of a pure ECL path and retiming circuits. The clock circuit reduces the chip latency fluctuation of the clock signal and absorbs the delay difference between the ECL clock and data through the CMOS circuits. A rerouting-Banyan (RRB) ATM switch, employing both the proposed converter circuits and the clock circuits, has been fabricated with 0.5 μm BiCMOS technology. The LSI, composed of CMOS 15 K gate logic, 8 Kb RAM, I Kb FIFO and ECL 1.6 K gate logic, achieved an operation speed of 704-MHz with power dissipation of 7.2 W  相似文献   
97.
To investigate the aging behavior of Li2+xTiO3+y, the materials Li2.0TiO3+y, Li2.2TiO3+y and Li2.4TiO3+y were stored under moist air at ambient temperature. Under these conditions the weight of Li2.0TiO3+y increased by only 1% in 100 days, which provides evidence for the high chemical stability of Li2TiO3. In comparison, under the same storage conditions the weights of Li2.2TiO3+y and Li2.4TiO3+y increased by 14% and 18%, respectively. The observed weight gain is attributed to the uptake of water, and to the reaction of a by-product of Li2+xTiO3+y with water and carbon dioxide. The weight gain curves were evaluated by the Jander equation. From the analysis, it was possible to obtain the diffusion coefficient of water through Li2TiO3 particles at ambient temperature. The determined value was 2 × 10−17 m2/s.  相似文献   
98.
The concept of dispositional resistance to change has been introduced in a series of exploratory and confirmatory analyses through which the validity of the Resistance to Change (RTC) Scale has been established (S. Oreg, 2003). However, the vast majority of participants with whom the scale was validated were from the United States. The purpose of the present work was to examine the meaningfulness of the construct and the validity of the scale across nations. Measurement equivalence analyses of data from 17 countries, representing 13 languages and 4 continents, confirmed the cross-national validity of the scale. Equivalent patterns of relationships between personal values and RTC across samples extend the nomological net of the construct and provide further evidence that dispositional resistance to change holds equivalent meanings across nations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
99.
In the present study, we investigated whether infants' own visual experiences affected their perception of the visual status of others engaging in goal-directed actions. In Experiment 1, infants viewed video clips of successful and failed goal-directed actions performed by a blindfolded adult, with half the infants having previously experienced being blindfolded. The results showed that 12-month-old infants who were previously blindfolded preferred to look longer at the demonstrator's successful actions, whereas no such preference was observed in 8-month-old infants. In Experiment 2, infants watched the same 2 actions when the adult demonstrator was not blindfolded. The responses of 12-month-old infants were the opposite of those observed in Experiment 1: They showed a preference for the failed actions. These findings suggest that previous experience influenced the subsequent perception of others' goal-directed actions in the 12-month-old infants. We favor the interpretation that the preference for the successful actions in the 12-months-old infants provided with blindfolded experience demonstrates the influence of perceptual experience on considering the visual status of others engaging in goal-directed actions. (PsycINFO Database Record (c) 2011 APA, all rights reserved)  相似文献   
100.
This paper proposes an inductive synthesis algorithm for a recursive process. To synthesize a process, facts, which must be satisfied by the target process, are given to the algorithm one by one since such facts are infinitely many in general. When n facts are input to the algorithm, it outputs a process which satisfies the given n facts. And this generating process is repeated infinitely many times. To represent facts of a process, we adopt a subcalculus of μ-calculus. First, we introduce a new preorder d on recursive processes based on the subcalculus to discuss its properties. pd q means that pf implies qf, for all formulae f in the subcalculus. Then, its discriminative power and relationship with other preorders are also discussed. Finally, we present the synthesis algorithm. The correctness of the algorithm can be stated that the output sequence of processes by the algorithm converges to a process, which cannot be distinguished from the intended one (if we could know it) by a given enumeration of facts, in the limit. A prototype system based on the algorithm is stated as well.  相似文献   
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