PURIFICATION AND PROPERTIES OF TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM MOBARAENSE

Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn²⁺ and NEM, and not affected obviously by Ba²⁺, Ca²⁺, Co²⁺, Cu²⁺, Fe²⁺, Fe³⁺, Mg²⁺, Mn²⁺, Na⁺ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases.     

粉煤灰降失水水泥浆在天然气井固井中的研究与应用

长庆天然气田属于低压、低渗透气田,存在承压能力较低的地层,固井设计要求全井段封固,并且采用双级双凝施工工艺。但由于用于低密度水泥浆中的漂珠生产量降低,价格上升,研究应用替代产品是固井技术发展的方向。在一级领浆中研究应用了粉煤灰水泥浆体系,其性能和固井质量可完全满足固井要求。粉煤灰水泥浆体系价格低和固井质量优良,使其成为漂珠水泥浆体系的替代配方。     

一种前馈型全摆幅恒跨导准恒增益CMOS运放设计

设计了一种适合在低电源电压下工作的前馈型输入级放大结构，在全摆幅的动态工作范围内，输入级跨导保持不变，采用负载电流补偿以保证增益近似恒定，输出采用前馈型AB类输结构，实现全摆幅输出。     

基于Delphi的报童模型的实现与动态仿真

已往库存论中的决策模型,它是把现实过程用数学的方法进行分析,得到决策值,但不能看到决策的过程,不能反映出现实生活中的不确定性、动态性。运用Delphi 7.0和SQL server 2000对销售过程进行了动态仿真,利用Newsboy模型的推广(S,s)订购策略来监控库存并做出决策,这样就体现出此过程的不确定性、动态性;且举出了一个仿真商品销售的实例,对其中的一些关键技术,如何仿真、算法的实现等进行了阐述。     

实用户外视频监控异常检测策略

树枝叶等植物的晃动是造成户外监控背景复杂性的主要因素。该文提出一种基于视频分析的运动对象检测策略，利用快慢双背射模型检测运动变化像素，并通过邻域像素的棚关性检测消除虚假运动对象。实验表明此算法对户外监控不但速度快而且检测效果理想，满足实用化要求。     

Tracking Control and Synchronization for Two-Dimension Discrete Chaotic Systems

1　ＩｎｔｒｏｄｕｃｔｉｏｎＳｉｎｃｅ 1 990 ,ｃｈａｏｔｉｃｓｙｎｃｈｒｏｎｉｚａｔｉｏｎ[1～ 4] ａｎｄｃｈａｏｔｉｃｃｏｎｔｒｏｌｌｉｎｇ[5～6] ｈａｖｅｒｅｃｅｉｖｅｄａｇｒｅａｔｄｅａｌｏｆａｔｔｅｎｔｉｏｎ[7～1 1 ] ,ａｎｄｃｈａｏｔｉｃｓｙｎｃｈｒｏｎｉｚａｔｉｏｎｉｓａｐｈｅｎｏｍｅｎｏｎｏｆｉｎｔｅｒｅｓｔｉｎｆｉｅｌｄｓｆｒｏｍｅｌｅｃｔｒｏｎｉｃｓｔｏｃｏｍｍｕｎｉｃａｔｉｏｎ[1 2 ] ,ａｎｄｆｒｏｍｂｉｏｐｈｙｓｉｃｓｔｏｎｅｕｒｏ ｓｃｉｅｎｃｅ[1 3] .Ｉｆｃｈａｏｔｉｃｓｙｎｃ…     

一种新的机器人手臂控制方法

从机器人手臂的关节电机力学出发，本文提出了一种独立于现行机器人关节控制器的离线补偿方法，通过对－２自由度机器人的数值研究，证明该法不仅能有效地克服由于惯性负载，关节间耦合与重力效应给控制系统带来的动态精度问题，而且还可以消除由于控制系统本身的固有特性所决定的系统的跟踪误差。