高效液相色谱-串联质谱法测定母乳中的 N-乙酰神经氨酸

目的建立高效液相色谱-串联质谱(HPLC-MS/MS)法测定母乳中N-乙酰神经氨酸的分析方法。方法用盐酸对样品进行酸水解释放出母乳中的结合态N-乙酰神经氨酸,高速离心后,采用C_(18)柱进行分离。以0.1%甲酸-乙腈溶液和0.1%甲酸-水溶液作为流动相进行梯度洗脱,电喷雾质谱检测,正离子多反应监测模式(MRM)进行定性和定量分析。结果在优化的条件下,N-乙酰神经氨酸在0.01~0.5 mg/L范围线性关系良好(r=0.9997)。以10、50、100 mg/kg 3个添加水平进行加标回收试验,N-乙酰神经氨酸的平均回收率为95.0%~98.7%,相对标准偏差为11.6%~14.5%,N-乙酰神经氨酸的检出限为0.21μg/kg。结论该方法简单、快速、重复性好、灵敏度高,可广泛用于母乳中N-乙酰神经氨酸的测定。     

页岩油评价中的若干关键问题及研究趋势

页岩储层的致密性限制了石油在其中的可动性、可动量,这是制约其勘探开发成效的瓶颈因素。而页岩油的可动性、可动量首先与页岩孔隙、喉道、裂缝及矿物组成密切相关,同时还与油-岩相互作用密切相关。前者事关页岩成储的可能性和机理,后者则事关石油在页岩中的赋存机理。因此,页岩的成储机理、石油在页岩中的赋存机理和石油在页岩中的可动性是页岩油研究中的3个关键科学问题;页岩油的富集性与其有机非均质性有关,页岩油的可采性/可压裂改造性与其无机非均质性有关,这是筛选页岩油“甜点”面临的2个关键技术问题。认识这3个关键的科学问题和2个关键的技术问题并建立定量的表征/评价技术是提高页岩油勘探开发成效的关键基础。结合近期的研究和国内外的成果,探讨了页岩油相关的研究现状和趋势,以期能够对推动页岩油理论研究的深入和勘探开发的实质性进展有所裨益。     

BP神经网络在多位置捷联寻北系统中的应用

为了精确拟合多位置捷联寻北系统采集的数据的曲线,计算陀螺初始位置和真北方向的夹角,简要介绍了多位置捷联寻北系统的工作原理,推导了寻北测量的数学模型,并分析了影响测量精度的因素,分别采用了最小二乘法和BP神经网络法,对两种方法的拟合精度和最终计算得到的寻北结果进行了比较。实验结果表明：与最小二乘法相比,BP神经网络拟合精度较高,拟合残差和较小,达到0.06位,残差的均方差达到34.45位,在计算相位角时,多次寻北结果的均值基本一致,但均方差明显由于最小二乘法,达到8″。满足寻北系统对数据拟合精度的要求。     

新型35kV快速跌落式熔断器的研制与应用

以河南油田为例,分析了35kV跌落式熔断器运行中存在的问题,介绍了加装隔弧挡板等几种解决方案,提出了35kV跌落式熔断器的改进方案,并通过试验和现场运行验证了实施效果。     

Reciprocating Probe Measurements of L-H Transition in LHCD H-Mode on EAST

As the power available in the initial phase of the ITER operation will be limited, accessing the high confinement mode (H-mode) with low heating power will be a critical issue. In the recent experiment on EAST, the H-mode was obtained for the first time with lower hybrid current drive (LHCD) wave only. Reciprocating Langmuir probe measurements at the outer midplane showed that the electron density n e and electron temperature T e in the scrape-off layer (SOL) were significantly reduced in the ELM-free phase, resulting in the increase of lower-hybrid wave (LHW) reflection. It was found that the power loss P loss was comparable during the L-H transition, by comparing the adjacent L-mode and H-mode discharge. The Dα emission, T e and n e decreased rapidly in the time scale of about 1 ms, and the radial electric field E r turned positive in this process near the last closed flux surface. Multiple L-H-L transitions were observed during a single shot when the applied LHW power was marginal to the threshold. The floating potential (Vf ) had negative spikes corresponding with the Dα signal, and E r oscillation evolved into several intermittent negative spikes just before the L-H transition. In some shots, dithering was observed just before the L-H transition.     

Radiative Divertor Plasma Behavior in L- and H-Mode Discharges with Argon Injection in EAST

Introducing strong radiative impurities into divertor plasmas has been considered as an important way to mitigate the peak heat load at the divertor target plate for ITER, and will be employed in EAST for high power long pulse operations. To this end, radiative divertor experiments were explored under both low (L) and high (H)-mode confinement regimes, for the first time in EAST, with the injection of argon and its mixture (25% Ar in D 2 ). The Ar injection greatly reduced particle and heat fluxes to the divertor in L-mode discharges, achieving nearly complete detached divertor plasma regimes for both single null (SN) and double null (DN) configurations, without increasing the core impurity content. In particular, the peak heat flux was reduced by a factor of ～6, significantly reducing the intrinsic in-out divertor asymmetry for DN, as seen by both the new infra-red camera and the Langmuir probes at the divertor target. Promising results have also been obtained in the H-modes with argon seeding, demonstrating a significant increase in the frequency and decrease in the amplitude of the edge localized modes (ELMs), thus reducing both particle and heat loads caused by the ELMs. This will be further explored in the next experimental campaign with increasing heating power for long pulse operations.     

淮北矿区多次跨采巷道破坏特征及控制对策研究

针对淮北矿区多次跨采巷道经反复维修仍无法满足正常使用的现状,以芦岭矿Ⅱ 82人行上山为研究对象,通过对巷道工程地质条件的综合分析,总结了该类巷道4种典型的破坏特征,即金属网压茬处破坏、底鼓、棚腿内扎、U型棚非对称破坏.采用数值模拟和理论分析方法研究了此类巷道的变形破坏机理,提出了包括新型中空注浆锚索、高性能超强锚杆和围岩注浆加固的巷道围岩强化控制技术.实践表明,采用中空注浆锚索与高性能锚杆相结合的组合控制技术可有效控制多次跨采巷道的剧烈变形,上方工作面跨采结束后巷道顶底移近量为126～393 mm,经简单维修即可满足正常使用.     

面向中小制造型企业的资源共享平台关键技术研究

为实现制造型企业运营所需的各类异构资源的优化配置和共享,研究了资源描述、评价和配置等关键技术,为资源共享平台的构建提供了一种解决方案。首先,根据制造型企业运营特点和资源需求,提出了平台系统的基本功能框架,设计了资源模型、资源评估和匹配度计算模型以及资源配置和管理工作流程。最后构建了平台结构框架。     

累托石对水中扑尔敏的吸附性能

对累托石对水中扑尔敏的吸附性能进行了研究,系统地研究了吸附时间、溶液浓度、pH值对其吸附行为产生的影响。实验结果表明:累托石对扑尔敏的吸附速率先快速增大后趋于平缓,反应系统达到吸附平衡的时间约为22 h;在等温吸附研究中,室温下累托石对扑尔敏的最大吸附量为121.76 mg/g,吸附等温线方程符合Langmuir等温模型;吸附行为符合拟二级动力学方程;在pH值为2～8时,吸附量随pH值的升高而增大。     

内蒙古北山造山带东段虎头山北地区地质构造特征及铜金找矿方向探讨

北山造山带是我国西北重要的矿集区,虎头山北地区位于该区东段,是寻找铜、金多金属矿床的有利区域。作者以板块构造理论和成矿系列理论为依据,根据区域典型矿床和测区铜金矿床的对比研究,总结了测区矿床的时空展布特征、成矿和演化规律,对测区控矿条件和找矿方向进行了讨论。测区古元古界北山岩群地层和志留系公婆泉组是铜、金矿化的主要含矿层位,燕山期花岗岩同期含矿热液是矿液来源,F1断裂不仅创造了容矿空间,为成矿热液提供了通道,也是重要的成矿控矿构造。结合区内物化探特征,在测区圈出4个成矿远景区和4个优选找矿靶区。