Determination of aniline derivatives in oils related to the toxic oil syndrome by atmospheric pressure ionization-tandem mass spectrometry

In 1981, an unknown disease appeared in Spain, the Spanish Toxic Oil Syndrome. Nowadays and despite all efforts, the etiological agent is still unknown. Early studies showed a link between this illness and the consumption of denatured rapeseed oil fraudulently processed and marketed as edible oil. Two families of aniline derivatives present in these oils (fatty acid anilides and acylated phenyl amino propanediol derivatives or PAPs) were found to be good chemical markers of toxic oils. In this work, a new method has been developed to analyze these aniline derivatives in oil samples by HPLC-MS and HPLC-MS/MS with an API source. For their quantification, three different internal standards were used, one for anilides and two for PAPs. Quantification limits were 8 ppm for anilides and 0.2 ppm for PAPs. Anilides and PAPs were found in marker-positive samples at levels up to 50,000 and 330 ppm, respectively. The relative abundance of the different fatty acid anilides and PAPs correlates with the fatty acid composition of the oils. More than 2,600 different samples were analyzed by this method in the most exhaustive screening of suspected toxic oils carried out to date.     

Contribution of starter culture to the sensory characteristics of fermented Almagro eggplants

Trial fermentations were performed using three experimental starter cultures with a view to selecting the most appropriate starter for use in the manufacture of Almagro eggplants. The lactic acid bacterial strains used in the starters had previously been isolated from spontaneous fermentations. The combined action of the obligate heterofermentative species Lactobacillus brevis and the facultative heterofermentative species L. plantarum yielded Almagro eggplants with sensory properties preferred by panelists. Conversely, another obligate heterofermentative species, namely, L. fermentum, present in certain starter formulations tested, appeared to encounter difficulty growing during fermentation and exerted little influence on the sensory characteristics of the eggplants produced.     

n-Alkane content of intramuscular lipids of Iberian fresh ham from different feeding systems and crossbreeding

n-Alkane content of intramuscular lipids (Biceps femoris muscle) of the Iberian pig have been determined. Thirty-four pigs, divided into four groups, based in the feeding system (Montanera, fed on acorns and pasture; and Pienso, fed on a concentrate feed) and in the genotype (Iberian pure pigs; and Iberian crossbred with Duroc 50%) were studied. n-Alkane content of intramuscular lipids has not been affected by neither crossbreeding nor feeding, although the analysis of feeds administered to the pigs showed greater n-alkane values in pasture (consumed by animals in montanera), than in acorns and concentrate feed.     

新型单摄像机镜像双目视觉传感器的研究

通过分析玻璃厚度激光折射测量原理误差,并以双目立体视觉传感器三维测量模型为基础,提出一种自动补偿玻璃板上下表面倾斜的单像机双目视觉传感器模型.变传统的双CCD摄像机结构为单CCD摄像机结构,加装一组对称光学反射镜,镜像出一对虚拟摄像机,可在同一个CCD像面上采集到存在视差的两幅图像,从而消除玻璃板上下表面倾斜角引起的系统误差;建立了标定数学模型,并确定视觉传感器测量模型的标定方法.     

微型多槽道平板热管传热特性分析及最大传热量预测

对三角形槽道的微型平板热管,进行流动、传热性能的理论分析和模拟.建立了微型平板热管的一维稳态模型,以分析工质在热管内的轴向分布,液体和蒸汽压力、流速的轴向变化,并计算其最大传热量.该模型更精确地考虑了具有互相连通蒸汽空间的平板热管,汽液界面剪切摩擦力对其传热性能影响,模型预测结果与实验数据取得较好的符合,能够更准确地预测微热管的最大传热量.该模型可用来帮助微型多槽道平板热管的结构设计.     

茂金属耐热聚乙烯管材料的结构与性能

对3种茂金属耐热聚乙烯（PE-RT）管材料（ M 1,M 2和M 3）的基本物性、热性能、相对分子质量及其分布、力学性能、流变性能等进行分析表征,考察了3种PE-RT管材料的性能差异。结果表明: 3种PE-RT管材料均属于中密度聚乙烯产品,熔体流动速率相近;3种PE-RT管材料中,国产料M 1,M 2的分子质量分布较宽,熔点较低,结晶度小,韧性较好;进口料M 3的熔体拉伸强度较大,可拉伸性最佳,弯曲弹性模量高,具有良好的刚性。     

Kinetic study of ozonation of molasses fermentation wastewater

A kinetic study of molasses wastewater ozonation was carried out in a stirred tank reactor to obtain the rate constants for the decolorization reaction and the regime through which ozone is absorbed. First, fundamental mass transfer parameters such as ozone solubility, volumetric mass transfer coefficients and ozone decomposition kinetics were determined from semi-batch experiments in organic-free solutions with an ionic composition similar that of industrial wastewater. The influence of operating variables such as the stirring rate and gas flow rate on the kinetic and mass transfer parameters was also studied. The application of film theory allows to establish that the reactions between ozone and colored compounds in wastewater take place in the fast and pseudo-first-order regime, within the liquid film. The decolorization rate constants were evaluated at pH 8.7 and 25 degrees C, varying from 0.6 x 10(7) to 3.8 x 10(7)L mol(-1)s(-1), depending on the stirring rate and the inlet gas flow.     

基于HSI空间彩色差的快速水平集分割方法*

为提高分割速度、改善分割效果,从两方面进行改进:首先对传统的快速水平集分割进行改进,得到基于CV模型的快速水平集;其次,通过引入HSI空间中的彩色差概念,建立一种新的、尤其适合彩色图像的快速水平集分割方法。实验表明,新提出的基于HSI彩色差的快速水平集分割方法,对彩色图像具有良好的分割效果。与传统水平集分割相比,新方法在分割性能和运算时间两方面均有明显提高。     

基于运动图像序列的异常行为检测*

针对公共重点区域的智能监视问题,研究了一种基于运动历史图像（motion history image,MHI）的行人异常行为检测方法。利用运动图像序列得到的MHI获取视频帧中运动目标的运动方向,由运动方向的变化分类确定人体运动模式和行为是否异常,同时给出相应的实验结果。结果表明,该方法实现简单,具有较好的实时性与鲁棒性,可以作为实时监控系统中异常行为检测的有效方法。     

Transepithelial transport across Caco-2 cell monolayers of antihypertensive egg-derived peptides. PepT1-mediated flux of Tyr-Pro-Ile

This paper examines the in vitro transepithelial transport of antihypertensive peptides derived from egg proteins using Caco-2 cell monolayers. Ovokinin (FRADHPFL) was absorbed intact through the Caco-2 cell epithelium, although it was also susceptible to the action of brush-border aminopeptidases that yielded shorter fragments prior to their transport. The tripeptide YPI was resistant to cellular peptidases and transported through the monolayer, what suggests that the reduction in systemic blood pressure caused by this peptide may be mediated by effects at tissue level. Its pathway for transepithelial absorption was examined using inhibitors of the different mechanisms for oligopeptide transport in the intestinal tract. The main route involved in the transepithelial flux of YPI is probably the peptide H(+)-coupled transporter PepT1. These results highlight the potential of antihypertensive peptides to be used in the formulation of functional foods.