Rapid, sensitive procedure to determine buspirone levels in rat brains using gas chromatography with nitrogen-phosphorus detection

Reported here is a rapid, sensitive and relatively inexpensive procedure using gas chromatography with nitrogen-phosphorus detection (GC-NPD) to quantify buspirone levels in brains of rats. The analyte was directly extracted from brain homogenate with toluene after basification and then subjected to GC-NPD analysis using a capillary column. The calibration curves were linear over the range of 10 to 320 ng per 2 ml of brain homogenate, with typical r2 values >0.99. The assay was highly reproducible and gave peaks with excellent chromatographic properties.     

Patients with rheumatoid arthritis: study of the correlation between density of glucocorticoid receptors in synovial cells and clinical response to steroidal treatment

BACKGROUND: The target cellular response to glucocorticoids is proportional to the concentrations or affinity of specific receptors to these substances. AIM: To look for a correlation between glucocorticoid receptor concentrations in synovial wall cells and the clinical response to steroidal treatment in patients with rheumatoid arthritis. PATIENTS AND METHODS: Twenty eight patients with rheumatoid arthritis were studied. Each subject was subjected to a synovial biopsy, in which a dry radioautographic technique for diffusible compounds was used. Patients were treated afterwards with three 500 mg intravenous pulses of methilprednisolone. RESULTS: A mean of 44.8% of synovial cells (range 30.1-62.8%) had binding sites for 3H dexamethasone. All patients had a significant clinical improvement after methylprednisolone. Multiple regression analysis did not show a correlation between clinical response and glucocorticoid receptor concentration. CONCLUSIONS: The lack of association between glucocorticoid receptor concentrations and clinical response could be due to the large steroid dose used, that saturated all available receptors.     

Physiologo-biochemical characteristics if Gluconobacter oxydans and prospects for its use in biotechnology and biosensor systems (review)

Gluconobacter oxydans possesses a unique organization of metabolic systems, which are characterized by reduction of major dissimilation pathways, surface localization of main oxidative enzymes responsible for partial oxidation of carbon substrates, high performance of electron-transport chains, and accumulation of partially oxidized metabolites in the medium. These features allow us to use the cells of these microorganisms in biotechnology for production of several food products and medicines. The use of G. oxydans in biosensors for estimation of concentrations of sugars, aldoses and polyalcohols is promising. Physiological and biochemical features of these microorganisms enabling their use in biotechnology and receptor elements of biosensors are reviewed.     

基于受控时间Petri网的车辆检测过程模型

针对汽车检测线运行效率评估及确保给定数量车辆检测时间最短的问题,通过分析检测过程中的驱动事件和状态演变,给出了车辆调度的约束条件,在此基础上把模型中的时间约束与受控Petri网相结合,提出了受控时间Petri网（Controlled Time Petri Nets）模型,采用该模型对检测线调度过程中的节拍控制与车辆调度进行可视化的建模与分析。实践表明对于多批次车辆检测任务采用该过程模型可明显缩短检测时间。     

OPNET环境下的PIM—SM组播路由协议仿真

IPv6组播技术作为下一代互联网的核心技术之一,相对于IPv4组播技术具有更大的优越性。文章首先对PIM-SM组播协议在IPv6上实现PIM-SM涉及的问题进行了论述,然后在OPNET网络仿真模型中实现了对IPv6和IPv4混合网络场景的配置,进行组播的仿真实验比较单播与组播网络的性能,系统地分析了PIM-SM协议在OPNET中的实现。     

基于加权决策树的蛋白质序列分类算法研究

针对蛋白质序列分类的需求,深入研究了蛋白质序列分类算法。对蛋白质序列的特征属性进行了大量的分析和研究,给出了蛋白质序列特征属性的描述形式。在此基础上设计了一种基于加权决策树的蛋白质序列分类算法,详细阐述了加权决策树的构造过程以及决策树的主要参数计算方法,而且根据蛋白质序列的特征,对决策树进行了改进,给出了加权决策树的实现方法。测试结果表明：设计的蛋白质序列分类算法具有较高的分类精度和较快的执行速度。     

Software structure evaluation based on the interaction and encapsulation of methods

Software structure is the most important factor affecting the quality of a program.The evaluation of software structure is a foundational technique for software development.However,the traditional evaluation criteria,such as "high cohesion and low coupling",are no longer appropriate for the new web applications,which are designed with a hierarchical framework.A novel approach is proposed in this paper to evaluate software quality based on the interaction and encapsulation of methods.In particular,two criteria for high quality software,the functional and hierarchical structure,are proposed to support widely used web applications.The cohesion and coupling and layer matching ratios between methods within a software package are calculated to determine whether the package is well implemented in terms of functional or hierarchical structure.This approach enables us to quantify the quality of a software system according to the structural types of its packages.Experiments were carried out on 18 versions of Struts and six open-source software packages.Our evaluation concurs with the intuition that minor upgrades,aimed at fixing bugs and adding minor functionality quickly,reduce software quality,whereas major upgrades,which normally clean up messy code and re-construct the software,improve software quality.     

石化行业自动化控制仪表常见故障分析及其处理

介绍化工生产过程中仪表故障及其处理措施。     

基于去调频技术的斜视聚束SAR成像方法

针对具有方位谱混叠现象的斜视聚束式合成孔径雷达(SAR)成像问题,本文研究了一种基于去调频技术的成像方法.文中首先分析了斜视聚束SAR中的方位谱混叠现象,从理论上找出了斜视角影响方位粗聚焦的根本原因.基于分析结果,引入了回波信号非线性相位校正预处理法,彻底消除了斜视角的影响,从而得到正确的粗聚焦结果.然后基于斜视成像模型,推导了预处理回波信号的方位粗聚焦和精聚焦处理过程.在精聚焦的方位滤波处理中,针对非线性相位校正预处理导致的场景聚焦深度限制问题,提出了一种改进的非线性调频变标(NLCS)算法,实现了回波信号的高精度方位压缩.仿真实验结果证明了文中理论分析的正确性和所提方法的有效性.