Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein

A universal method that improves protein stability and evolution has thus far eluded discovery. Recently, however, studies have shown that insertional fusion to a protein chaperone stabilized various target proteins with minimal negative effects. The improved stability was derived from insertion into a hyperthermophilic protein, Pyrococcus furiosus maltodextrin‐binding protein (PfMBP), rather than from changes to the target protein sequence. In this report, by evaluating the thermodynamic and kinetic stability of various inserted β‐lactamase (BLA) homologues, we were able to examine the molecular determinants of stability realized by insertional fusion to PfMBP. Results indicated that enhanced stability and suppressed aggregation of BLA stemmed from enthalpic and entropic mechanisms. This report also suggests that insertional fusion to a stable protein scaffold has the potential to be a useful method for improving protein stability, as well as functional protein evolution.     

A highly efficient polymer‐anchored palladium(II) complex catalyst for hydrogenation,Heck cross‐coupling and cyanation reactions

BACKGROUND: Precise architectures of steric and electronic properties of palladium species play a crucial role in designing highly functionalized catalyst systems responsible for target organic transformations. Pd catalysts supported on polymer materials have been employed extensively as catalysts not only for hydrogenation but also for coupling reactions in the production of fine chemicals. RESULTS: A new polymer‐anchored Pd(II) complex has been synthesized and characterized. The catalyst shows high catalytic activity in the hydrogenation of styrene oxide, Heck cross‐coupling and cyanation reactions of aryl halides. The effect of various reaction parameters were investigated to optimize reaction conditions. The catalytic system shows good activity in the hydrogenation of styrene oxide (conversion 98%) with a selectivity to 2‐phenylethanol (93%) which is higher than its homogeneous analogues. The catalyst also exhibits excellent catalytic activity for the Heck cross‐coupling and cyanation reactions of various substituted and non‐substituted aryl halides. CONCLUSIONS: Results demonstrate that the catalyst is robust and stable and can be recovered quantitatively by simple filtration and reused several times without loss of activity. Copyright © 2010 Society of Chemical Industry     

A real-coding jumping gene genetic algorithm (RJGGA) for multiobjective optimization

This paper presents a real jumping gene genetic algorithm (RJGGA) as an enhancement of the jumping gene genetic algorithm (JGGA) [T.M. Chan, K.F. Man, K.S. Tang, S. Kwong, A jumping gene algorithm for multiobjective resource management in wideband CDMA systems, The Computer Journal 48 (6) (2005) 749-768; T.M. Chan, K.F. Man, K.S. Tang, S. Kwong, Multiobjective optimization of radio-to-fiber repeater placement using a jumping gene algorithm, in: Proceedings of the IEEE International Conference on Industrial Technology (ICIT 2005), Hong Kong, 2005, pp. 291-296; K.F. Man, T.M. Chan, K.S. Tang, S. Kwong, Jumping-genes in evolutionary computing, in: Proceedings of the IEEE IECON’2004, Busan, 2004, pp. 1268-1272]. JGGA is a relatively new multiobjective evolutionary algorithm (MOEA) that imitates a jumping gene phenomenon discovered by Nobel Laureate McClintock during her work on the corn plants. The main feature of JGGA is that it only has a simple operation in which a transposition of gene(s) is induced within the same or another chromosome in the genetic algorithm (GA) framework. In its initial formulation, the search space solutions are binary-coded and it inherits the customary problems of conventional binary-coded GA (BCGA). This issue motivated us to remodel the JGGA into RJGGA. The performance of RJGGA has been compared to other MOEAs using some carefully chosen benchmark test functions. It has been observed that RJGGA is able to generate non-dominated solutions with a wider spread along the Pareto-optimal front and better address the issues regarding convergence and diversity in multiobjective optimization.     

Three cases of comprehensive dietary therapy and pharmacotherapy of patients with complex obesity-related diseases

The effect of weight loss with anorectic medications on sleep apnea, non-insulin-dependent diabetes, and steatohepatitis is illustrated in three cases from practice in a clinical nutrition setting. Prevention of obesity, a chronic disorder, is preferable, but when obesity becomes a major obstacle in the care of patients with respiratory, cardiovascular, and metabolic disorders and osteoarthritis, an intense course of weight reduction using anorectic medications under medical and dietetic guidance is essential for patients' survival and reduction of medical cost.     

Development of a wind turbine model and simulation platform using an acausal approach: Multiphysics modeling,validation, and control

This article presents the development of the Control-oriented, Reconfigurable, and Acausal Floating Turbine Simulator (CRAFTS). CRAFTS has a modular, hierarchical model architecture that enables rapid and accurate simulation of wind turbines. The architecture facilitates the incorporation of model variants, and its system reconfiguration features help simulate multiple design variants. CRAFTS also supports the integration of models developed on a causality-free platform (e.g., Modelica®) with existing causal models. This article focuses on the validation of a land-based wind turbine models against OpenFAST, an industry-standard platform, for several test cases. Closed-loop scenarios are also tested using the standard ROSCO controller and compared against OpenFAST. In addition, a nonlinear controller developed in our prior work is also evaluated. The test cases demonstrate the user-friendly and computationally efficient capabilities of CRAFTS to facilitate control co-design, assist in incorporating multiphysics models, be adaptable to design variants, and allow for rapid simulations to validate models and evaluate controllers.     

Preparation and characterisation of novelty food preservatives by Maillard reaction between ε-polylysine and reducing sugars

In order to enhance the application scope and usage effect of ε-polylysine, it was modified by Maillard reaction through endowing antioxidant activity. Characterisations of different type of reaction systems were investigated. Results showed that with increased heating treatment time at constant 90 °C and UV-Vis absorbance at 294 and 420 nm, fluorescence intensity of modified products were increased. DPPH radical scavenging activity of modified products from D-glucose (D-Glc), D-fructose (D-Fru) and D-xylose (D-Xyl) was increased to 40.5%, 44.0% and 69.4%, and the reducing power was increased to 0.9, 1.0 and 1.3 respectively (P < 0.05). Moreover, compared with ε-polylysin, three types of modified products have similar antibacterial property. In addition, three types of modified products showed low cytotoxicity against the cultured human HepG2 cells in vitro. Therefore, the modified products derived from ε-polylysine model could be used as potential food preservatives.     

On thermodynamics of methane + carbonaceous materials adsorption

This study presents the theoretical frameworks for the thermodynamic quantities namely the heat of adsorption, specific heat capacity, entropy, and enthalpy for the adsorption of methane onto various carbonaceous materials. The proposed theoretical frameworks are developed from the rigor of thermodynamic property surfaces of a single component adsorbate–adsorbent system and by incorporating the micropore filling theory approach, where the effect of adsorbed phase volume is considered. The abovementioned thermodynamic properties are quantitatively evaluated from the experimental uptake data for methane adsorption onto activated carbons such as Maxsorb III at temperatures ranging from 120 to 350 K and pressures up to 25 bar. Employing the proposed thermodynamic approaches, this paper shows the thermodynamic maps of the charge and discharge processes of adsorbed natural gas (ANG) storage system for understanding the behaviors of natural gas in ANG vessel.