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A HPLC-fluorescence method, using the fluorophore SBD-F (ammonium-7-fluorobenz-2-oxa-1,3-diazole-4-sulfonate), was adapted for the quantification of metallothioneins and their isoforms from the Moroccan mussel Mytilus galloprovincialis. The method was first optimized using a rabbit liver metallothionein. The effects of EDTA, tris(2-carboxyethyl)phosphine, and SBD-F on the labeling efficiency were studied. The optimized method was then applied to evaluate the amount of metallothionein in the mussels either exposed to cadmium in the laboratory or collected from the Casablanca coast, Morocco. The concentrations of metallothioneins measured in the field samples describe the degree of contamination of the sites and are reflected by distinct isoform patterns.  相似文献   
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Two new algorithms for the fast calculation of the Vavilov distribution within the interval 0.01相似文献   
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A chemical method to graft carbon nanotubes onto a carbon fiber   总被引:2,自引:0,他引:2  
A simple method is developed for grafting carbon nanotubes (CNTs) onto a carbon fiber surface. CNT and carbon fiber undergo an oxidation treatment. Oxidation generates oxygen, like carboxyl, carbonyl or hydroxyl groups, or amine groups on nanotubes and carbon fiber surface. Functionalized CNTs are dispersed in a solvent and deposited on carbon fibers. The bonds between CNT and carbon fiber are operated by esterification, anhydridation or amidization of the chemical surface groups. The resulting materials are characterized by scanning electron microscopy (SEM). CNTs form a 3D network around the carbon fibers. Likewise, CNT bonding between two fibers is observed.  相似文献   
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The problem of mixed convection heat transfer inside a horizontal vented enclosure through the lower and upper parts, respectively, of its left and right vertical walls is studied numerically using Al2O3-water nanofluid as working fluid. The bottom wall is subjected to a linearly varying (increasing or decreasing) heating temperature profiles, while the other boundaries are considered thermally insulated. The fresh fluid is admitted from the bottom part of the left vertical wall by injection or by the suction imposed on the opening of the right vertical wall. Based on numerical predictions, the conjugate effect of the Reynolds number and the nanoparticle concentration on fluid flow and heat transfer characteristics is studied. The obtained results demonstrate clearly the positive role of the nanoparticles addition on the improvement of the heat transfer rate and the mean temperature within the cavity. In addition, the flow structure and the temperature distribution inside the cavity are seen to be very sensitive to the variations of the Reynolds number, the imposed external flow mode, and the heating type. Results presented show that, in general, the decreasing heating mode is more favorable to the heat transfer in comparison with the case of the increasing heating mode. The cooling efficiency is found to be more pronounced by the injection/suction mode by applying the increasing/decreasing heating type.  相似文献   
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The software‐defined networking (SDN) paradigm proposes to decouple the control plane (decision‐making process) and the data plane (packet forwarding) to overcome the limitations of traditional network infrastructures, which are known to be difficult to manage, especially at scale. Although there are previous works focusing on the problem of quality of service (QoS) routing in SDN networks, only few solutions have taken into consideration the network consistency, which reflects the adequacy between the decisions made and the decisions that should be taken. Therefore, we propose a network architecture that guarantees the consistency of the decisions to be taken in an SDN network. A consistent QoS routing strategy is then introduced in a way that avoids any quality degradation of prioritized traffic while optimizing resources usage. Thus, we proposed a traffic dispersion heuristic in order to achieve this goal. We compared our approach with several existing framework in terms of best‐effort flows average throughput, average video bitrate, and video quality of experience (QoE). The emulation results, which are performed using the Mininet environment, clearly demonstrate the effectiveness of the proposed approach that outperforms existing frameworks.  相似文献   
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