Species distribution,antibiotic resistance and virulence traits in enterococci from meat in Tunisia

Antimicrobial resistance and the mechanisms implicated were studied in 119 enterococci from 105 meat samples from Tunisian markets. Almost 24.5% of recovered enterococci showed resistance against four or more antimicrobial agents and these isolates were identified to the species level. Enterococcus faecalis was the most prevalent species (41%). High percentages of erythromycin and tetracycline resistances were found among our isolates, and lower percentages were identified to aminoglycosides, ciprofloxacin and chloramphenicol. All tetracycline-resistant isolates carried the tet(M) and/or tet(L) genes. The erm(B) gene was detected in 78.5% of erythromycin-resistant isolates, ant(6)-Ia gene in 58.8% of streptomycin-resistant isolates, and cat(A) gene in one chloramphenicol-resistant isolate. Forty-eight isolates carried the gelE gene and exhibited gelatinase activity. The hyl and esp genes were detected in one and three Enterococcus faecium isolates, respectively. Streptomycin-resistant isolates showed a high genetic diversity by PFGE and MLST. Meat might play a role in the spread through the food chain of enterococci with these virulence and resistance characteristics to humans.     

The unsteady pressure field and the aerodynamic performances of a Savonius rotor based on the discrete vortex method

The aim of this paper is to numerically explore the non-linear two-dimensional unsteady potential flow over a Savonius rotor and to develop a code for predicting its aerodynamics performances. In the model developed, the rotor is represented in a median plane by two semicircles, displaced along their common diameter. The two semicircles can be considered to produce lifting effects. As a result, they are modelled by a collection of discrete vortices on their contours. The flow field is then governed by the Laplace equation. The versatile Neumann boundary condition, applied over the contour of the semicircles and the Kutta Joukowsky condition applied at the four extremities of the semicircles have been used in the modelling. The torque distribution of the stationary rotor and the unsteady pressure field on the blades of the rotating rotor, predicted by the code developed, have been compared and validated by some experimental data.     

A prototype of a fuel cell PEM emulator based on a buck converter

After a brief introduction about fuel cell systems, and their modelling, this paper proposes a possible solution to emulate a proton exchange membrane fuel cell (PEM-FC) system by using a DC–DC buck converter. The fuel cell system, including all its auxiliaries and related control systems, is emulated by a buck converter realized experimentally and controlled in the DSPACE environment. The realization of the buck converter allows the behaviour of any fuel cells to be easily emulated since only the modification of the control law of the switch is necessary. The proposed emulator can be applied easily to other fuel cell systems if the polarization curve has the same current rate and maximum power. In this way it is possible to utilize the converter and perform the necessary tests to optimize a fuel cell system by avoiding the waste of hydrogen and the purchase of cells as well as any cell damage. With regard to current other types of emulators, the one presented here has the following characteristics: (1) all the auxiliaries of the system have been considered, each including its own control system, as in a real FCS, (2) the converter is a classical buck converter with a free-wheeling diode and is designed to have a high bandwidth and to be practically always in conduction mode (discontinuous mode appears only at very low currents) (3) the voltage control is made by a space-state controller, able to fix properly the closed loop poles of the system, thus guaranteeing the desired bandwidth of the control system and (4) it can be used in laboratory as a stand-alone low-cost system for design and experimental purposes.     

Method for reliable isolation of Lactobacillus sakei strains originating from Tunisian seafood and meat products

In Tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. Such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. We isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of Tunisia, and selectively searched for Lactobacillus sakei, a lactic acid bacterium potentially useful as starter or protective culture. Eighty six (86) strains were isolated from various seafood (anchovy, sardine, sole, mullet, and octopus), or meat (pork, veal, beef, sheep, chicken, and turkey) products that were either fresh, or transformed by different traditional processes. Several methods were used in order to develop a rapid and reliable protocol for the direct identification of L. sakei. Amplified ribosomal DNA restriction analysis (ARDRA) classified the various isolates into 9 distinct groups. Search for the presence of the L. sakei specific katA gene indicated that all positive isolates were grouped in the same ARDRA group. Sequencing of 16S rDNA confirmed those isolates as L. sakei. Those 22 different L. sakei strains represent 25.6% of the total isolates, while other isolates found in the different ARDRA groups were tentatively ascribed to Lactobacillus plantarum, Lactococcus lactis/garviae, Enterococcus avium, Streptococcus parauberis, Hafnia alvei, Pediococcus pentosaceus, and Lactobacillus curvatus through 16S rDNA sequencing. A fast and reliable method to isolate and discriminate L. sakei from complex food environments is proposed.     

Effect of Frictions on the Ballistic Performance of a 3D Warp Interlock Fabric: Numerical Analysis

3D interlock woven fabrics are promising materials to replace the 2D structures in the field of ballistic protection. The structural complexity of this material caused many difficulties in numerical modeling. This paper presents a new tool that permits to generate a geometry model of any woven fabric, then, mesh this model in shell or solid elements, and apply the mechanical properties of yarns to them. The tool shows many advantages over existing software. It is very handy in use with an organization of the functions in menu and using a graphic interface. It can describe correctly the geometry of all textile woven fabrics. With this tool, the orientation of the local axes of finite elements following the yarn direction facilitates defining the yarn mechanical properties in a numerical model. This tool can be largely applied because it is compatible with popular finite element codes such as Abaqus, Ansys, Radioss etc. Thanks to this tool, a finite element model was carried out to describe a ballistic impact on a 3D warp interlock Kevlar KM2? fabric. This work focuses on studying the effect of friction onto the ballistic impact behavior of this textile interlock structure. Results showed that the friction among yarns affects considerably on the impact behavior of this fabric. The effect of the friction between projectile and yarn is less important. The friction plays an important role in keeping the fabric structural stability during the impact event. This phenomenon explained why the projectile is easier to penetrate this 3D warp interlock fabric in the no-friction case. This result also indicates that the ballistic performance of the interlock woven fabrics can be improved by using fibers with great friction coefficients.     

Spectral Discretization of the Axisymmetric Vorticity,Velocity and Pressure Formulation of the Stokes Problem

We consider the Stokes problem in a three-dimensional axisymmetric bounded domain with non standard conditions which involve the normal component of the velocity and tangential component of the vorticity. We reduce the three-dimensional problem into a two-dimensional one and we write a variational formulation of it with three independent unknowns: the vorticity, the velocity and the pressure. Then we propose a discretization by spectral methods which relies on this formulation. A detailed numerical analysis leads to optimal error estimates for the three unknowns and numerical experiments confirm the interest of the discretization.     

Dynamic robust power allocation games under channel uncertainty and time delays

In this paper, we study dynamic robust power allocation strategies under the imperfectness of the channel state information at the transmitters. Considering unknown payoff functions at the transmitters, we propose an heterogeneous Delayed COmbined fully DIstributed Payoff and Strategy Reinforcement Learning (Delayed-CODIPAS-RL) in which each transmitter learns its payoff function as well as its associated optimal strategies in the long-term. We show that equilibrium power allocations can be obtained using the multiplicative weighted imitative CODIPAS-RLs and Bush-Mosteller based CODIPAS-RL. We also show almost sure convergence to the set of global optima for specific scenarios.     

Methane decomposition into COx-free hydrogen and carbon nanomaterials over ZrO2–M (M = MgO,Al2O3, SiO2, La2O3 or CeO2) binary oxides supported cobalt catalysts

Hydrogen production via methane decomposition has attracted great attention as a source of clean energy. In this work, the catalytic decomposition of undiluted methane into CO_x-free hydrogen and carbon nanomaterial over ZrO₂–M (M?=?MgO, Al₂O₃, SiO₂, La₂O₃ and CeO₂) binary oxides supported Co catalysts was studied. The fresh and spent catalysts were characterized by XRD, H₂-TPR, N₂ adsorption–desorption, TEM and Raman spectroscopy. The results revealed that the incorporation of secondary oxide to ZrO₂ support played a vital role in the activity and stability of cobalt metal. The activity results illustrated that Co/Zr–Mg exhibited better activity in terms of hydrogen yield compared with the other Zr–M supported catalysts. This is ascribed to the moderate cobalt oxide-support interaction and forming CoMgO_x species which enhance the Co₃O₄ dispersion and prevent its aggregation on the catalyst surface. On the other hand, the Co/Zr–Si catalyst showed the lowest activity due to the agglomeration of Co₃O₄ on the surface of the SiO₂ support. High-resolution TEM images illustrated that almost the deposited carbon on the surface of spent catalysts was in the form of multi-walled carbon nanotubes.     

Ability of the Performance Criteria to Assess and Compare Reservoir Management Approaches

Water Resources Management - A reservoir management approach requires, regularly, an update of its parameters in order to assure that the reservoir continue to fulfill its role without major...