EPA or DHA Supplementation Increases Triacylglycerol,but not Phospholipid,Levels in Isolated Rat Cardiomyocytes

It is well recognized that a high dietary intake of long-chain polyunsaturated fatty acids (LC-PUFA) has profound benefits on health and prevention of chronic diseases. In particular, in recent years there has been a dramatic surge of interest in the health effects of n-3 LC-PUFA derived from fish, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Notwithstanding, the metabolic fate and the effects of these fatty acids once inside the cell has seldom been comprehensively investigated. Using cultured neonatal rat cardiomyocytes as model system we have investigated for the first time, by means of high-resolution magic-angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy in combination with gas chromatography (GC), the modification occurring in the cell lipid environment after EPA and DHA supplementation. The most important difference between control and n-3 LC-PUFA-supplemented cardiomyocytes highlighted by HR-MAS NMR spectroscopy is the increase of signals from mobile lipids, identified as triacylglycerols (TAG). The observed increase of mobile TAG is a metabolic response to n-3 LC-PUFA supplementation, which leads to an increased lipid storage. The sequestration of mobile lipids in lipid bodies provides a deposit of stored energy that can be accessed in a regulated fashion according to metabolic need. Interestingly, while n-3 LC-PUFA supplementation to neonatal rat cardiomyocytes causes a huge variation in the cell lipid environment, it does not induce detectable modifications in water-soluble metabolites, suggesting negligible interference with normal metabolic processes.     

Rhodium‐Catalyzed Enantioselective Hydrogenation of (E)‐Enol Acetate Acids

The novel rhodium complex [Rh(S)‐Phanephos(cod)]‐catalyzed hydrogenation of disubstituted (E)‐enol acetate carboxylic acids is reported. The catalytic cycle works under 30 bar of hydrogen under conventional heating giving different 3‐acetoxy‐2,3‐disubstituted carboxylic acids with ee ≥90%. Hydrogenation occurred also under microwave dielectric heating without eroding the enantioselectivity but improving the overall efficiency of the process. With microwaves, hydrogen pressure and reaction time required for complete hydrogenation dropped to 5 bar and 30 min, respectively. The best performance of this catalyst under microwave irradiation was TON 100, TOF 196 h⁻¹ with ee 99% on a 6‐g scale.     

Anti- and pro-oxidant activity of Cichorium genus vegetables and effect of thermal treatment in biological systems

The antiradical activity of water soluble components in six vegetables belonging to the Cichorium genus, i.e., three cultivars of red intybus species var. silvestre (Treviso, Chioggia, Verona red chicories), a white intybus species var. foliosum (Belgian chicory), and two vegetables of the endivia species var. latifolium (escarole chicory) and var. crispum (“crispa” chicory), were studied using two biological systems consisting of: (1) microsome membrane rat hepatocyties in which oxidative damage was induced by CCl₄; (2) gram-positive bacterium, Staphylococcus aureus cultures, subjected to damage with cumene hydroperoxyde. The obtained results show that in both systems the red vegetables possess the strongest antioxidant properties and contain different antioxidant compounds whether at a low or high molecular weight, but only those of high molecular-weight (MW > 3500 Da) are able to act as antioxidants in all the used systems. The lower MW fraction (MW < 3500 Da) showed itself to be pro-oxidant in the microsome system. The effects of thermal treatments such as boiling, freezing and freeze-drying were also investigated.     

Screening for adolescent problem drinking: Validation of a multidimensional instrument for case identification.

This research tested the thesis that adolescent problem drinking may be manifest in any of four independent domains (measured by the 24-item screening instrument, the Adolescent Drinking Inventory: Drinking and You): drinking-related loss of control, social indicators, psychological indicators, and physical indicators. Clinical assessments of 264 adolescents referred for screening revealed that more than half of the variation in clinical assessment of problem drinking severity was captured by these four domains and that two of the four domains (loss of control and psychological indicators) made an independent contribution to clinical assessment. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Modeling the health care costs of geriatric inpatients.

This paper extends a method for modeling the survival of patients in hospitals to allow the expected cost to be estimated for the patients' accumulated duration of time in care. An extension of Bayesian network (BN) theory has previously been developed to model patients' survival time in hospitals with respect to the graphical and probabilistic representation of the interrelationships between the patients' clinical variables. Unlike previous BN techniques, this extended model can accommodate continuous times that are skewed in nature. This paper presents the theory behind such an approach and extends it by attaching a cost variable to the survival times, enabling the costing and efficient management of groups of patients in hospitals. An application of the model is illustrated by considering a group of 4260 patients admitted into the geriatric department of a U.K. hospital between 1994-1997. Results are derived for the distribution for their length of stay in the hospital and associated costs. The model's practical use is highlighted by illustrating how hospital managers could benefit using such a method for investigating the influence of future decisions and policy changes on the hospital's expenditure.     

Dominic I: Progress toward domain independence in design by iterative redesign

This paper describes the first working version of a program called Dominic that performs design by iterative redesign in a domain-independent manner. The paper describes in detail the program's strategy, which stresses the concept of redesign dependencies to guide its redesign process. Dominic has been successfully tested in four different domains. Its performance on two of these (v-belt drive design and design of extruded heat sinks) is presented here. The redesign class of design problems on which Dominic works is that large class of problems that are intellectually manageable and solvable without subdivision into smaller parts. This includes the various subproblems ultimately created when large complex problems are decomposed for solution. Dominic is a hill-climbing algorithm, similar in this respect to standard optimization methods. However, its problem formulation or input language is more flexible for some design applications than optimization techniques. Work is continuing on a Dominic II in an effort to overcome some of the limitations of Dominic.     

Accelerating Scientific Discovery Through Computation and Visualization III. Tight-Binding Wave Functions for Quantum Dots

This is the third in a series of articles that describe, through examples, how the Scientific Applications and Visualization Group (SAVG) at NIST has utilized high performance parallel computing, visualization, and machine learning to accelerate scientific discovery. In this article we focus on the use of high performance computing and visualization for simulations of nanotechnology.     

Hydraulic Study of Multiple Inlet System: East Matagorda Bay, Texas

The hydrodynamic feasibility of a proposed third inlet to East Matagorda Bay, Texas, was examined by application of a two-dimensional, depth-averaged hydrodynamic model. Wind-driven flows in this remote, shallow bay frequently dominate the weak astronomical tide. The bay presently has two connections to the Gulf of Mexico, one through a short flood-relief channel and the other through a long and circuitous navigation channel. The study had to consider whether installation of a third inlet would cause the relief channel to close or increase the already strong current velocity in the navigation channel that is a concern for boating safety. The model was calibrated with measurements of wind, water level, and current taken in this study. It is concluded that the new inlet will be ebb-dominated because of the wind-induced current, and that the relief channel will not close in the presence of the new inlet. In addition, the peak current at a critical maneuvering area in the navigation channel that presently poses a hazard to vessel traffic will be reduced by as much as 25% as a result of opening the new inlet.     

Consolidation by spark plasma sintering of polyimide and polyetheretherketone

This article presents two high‐temperature thermoplastic powders which were sintered by spark plasma sintering in order to get homogeneous mechanical properties. Dense polyimide (PI) and polyetheretherketone (PEEK) specimens were obtained at temperatures as low as 320°C for PI and 200°C for PEEK, respectively. Relative densities higher than 99% were reached for both materials. In order to characterize their properties, in situ measurements with compression and hardness tests were carried out on sintered samples. This method allowed to obtain polymeric materials with improved mechanical properties. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40783.