Synergistic Processing of Skim Milk with High Pressure Nitrous Oxide,Heat, Nisin,and Lysozyme to Inactivate Vegetative and Spore-Forming Bacteria

Individual and combined effects of high pressure nitrous oxide (HPN₂O), heat, and antimicrobials on the inactivation of Escherichia coli, Listeria innocua, and Bacillus atrophaeus endospores in milk were all evaluated after 20-min treatments. Stand-alone milk treatments with HPN₂O (15.2 MPa), heat (45 and 65 °C), or nisin (50 and 150 IU mL^?1) resulted in log₁₀ reductions ranging only from 0.1 to 2.1 for E. coli and L. innocua. Combining HPN₂O (15.2 MPa) with heat (65 °C) inactivated 6.0 and 5.1 log₁₀ in the vegetative bacteria, respectively. Similarly, reductions of 5.9 and ≥ 6.0 log₁₀ of respective E. coli and L. innocua cells in milk were achieved through a combination of HPN₂O (15.2 MPa), heat (65 °C), and nisin (150 IU mL^?1). A 2.5 log₁₀ cycle inactivation of spores was obtained by HPN₂O, nisin (at both 50 and 150 IU mL^?1), and lysozyme (50 μg mL^?1) at 85 °C. Combining these processing techniques resulted in significantly greater microbial inactivation (p < 0.05) than the sum of individual reductions from each treatment alone, indicating synergistic effects. HPN₂O irrespective of processing temperatures did not cause any occurrence of sub-lethally injured cells or disruption in colloidal stability of milk at 65 and 85 °C (p ≥ 0.05). Color and pH changes in milk following the most demanding treatment conditions were minimal.     

Angiotensin Receptor-Neprilysin Inhibitor (ARNI) and Cardiac Arrhythmias

The renin-angiotensin-aldosterone system (RAAS) plays a major role in cardiovascular health and disease. Short-term RAAS activation controls water and salt retention and causes vasoconstriction, which are beneficial for maintaining cardiac output in low blood pressure and early stage heart failure. However, prolonged RAAS activation is detrimental, leading to structural remodeling and cardiac dysfunction. Natriuretic peptides (NPs) are activated to counterbalance the effect of RAAS and sympathetic nervous system by facilitating water and salt excretion and causing vasodilation. Neprilysin is a major NP-degrading enzyme that degrades multiple vaso-modulatory substances. Although the inhibition of neprilysin alone is not sufficient to counterbalance RAAS activation in cardiovascular diseases (e.g., hypertension and heart failure), a combination of angiotensin receptor blocker and neprilysin inhibitor (ARNI) was highly effective in several clinical trials and may modulate the risk of atrial and ventricular arrhythmias. This review summarizes the possible link between ARNI and cardiac arrhythmias and discusses potential underlying mechanisms, providing novel insights about the therapeutic role and safety profile of ARNI in the cardiovascular system.     

Control of cone-jet geometry during electrospray by an electric current

A new electrospray system has been successfully developed by employing a proportional–integral–derivative control action to maintain an electric current at a certain value. A polymer precursor solution containing polyethylene glycol was used to examine the performance of this system. The result showed that cone-jet geometry could be controlled easily by adjusting the electric current. The length of the cone-jet decreased as the electric current was increased, in a correlation that followed power law. We also found that the cone-jet observed during electrospraying was stable and robust with no disturbance during long periods of use (up to 4000 s). The present study is very useful for further development of high precision aerosol generators and particle synthesis.     

Ergonomics aspects of knob designs: a literature review

Hand-related apparatus designs that fail to accommodate appropriate hand postures can cause hand-related musculoskeletal disorders. While there have been studies on the handling and design of various hand-related apparatus, little has been written on the ergonomics aspects of knob designs. The aim of this paper is to review various knob designs and their ergonomics aspects. The literature review suggests that gearshift knobs, door knobs, gas valve knobs, butterfly nuts and screw knobs are common knobs used for daily living activities. Other knobs like the convex, knurled, spherical, cone-shaped and ridged knob are often used for industrial-related applications. The ergonomics considerations identified in knobs include aesthetic attraction, position, torque requirement and shape-coding. This literature review can be used as groundwork for the development of ergonomics theory and hand-related studies. Engineers can use this literature review to identify certain ergonomics aspects in knobs to improve equipment designs, operating efficiency and working comfort.     

A review of shape and size considerations in pinch grips

In handgrip-related studies, it has been theorised that modifying the design of object shapes and sizes to accommodate the hand can reduce risks of cumulative trauma disorders (CTDs). However, there appears to be a lack of theoretical groundwork to support this premise in the area of pinch grip research. Therefore, this article aims to provide a review of shape and size considerations in pinch grips. The literature reviews were first conducted individually on shape, size and pinch grip factors, followed by the development of a literature summary based on the theoretical connections among shape, size and pinch grips. Though further studies are still required, this review has provided fundamental knowledge on possible theoretical connections among shape, size and pinch grip factors. These preliminary efforts shed light on potential mechanisms that explain how shape and size can influence pinch grips towards preventing CTDs and maximising the human performance.     

Optimization of soy-biodiesel combustion in a modern diesel engine

As global petroleum demand continues to increase, alternative fuel vehicles are becoming the focus of increasing attention. Biodiesel has emerged as an attractive alternative fuel option due to its domestic availability from renewable sources, its relative physical and chemical similarities to conventional diesel fuel, and its miscibility with conventional diesel. Biodiesel combustion in modern diesel engines does, however, generally result in higher fuel consumption and nitrogen oxide (NO_x) emissions compared to diesel combustion due to fuel property differences including calorific value and oxygen content. The purpose of this study is to determine the optimal engine decision-making for 100% soy-based biodiesel to accommodate fuel property differences via modulation of air-fuel ratio (AFR), exhaust gas recirculation (EGR) fraction, fuel rail pressure, and start of main fuel injection pulse at over 150 different random combinations, each at four very different operating locations. Applying the nominal diesel settings to biodiesel combustion resulted in increases in NO_x at three of the four locations (up to 44%) and fuel consumption (11-20%) over the nominal diesel levels accompanied by substantial reductions in particulate matter (over 80%). The biodiesel optimal settings were defined as the parameter settings that produced comparable or lower NO_x, particulate matter (PM), and peak rate of change of in-cylinder pressure (peak dP/dt, a metric for noise) with respect to nominal diesel levels, while minimizing brake specific fuel consumption (BSFC). At most of the operating locations, the optimal engine decision-making was clearly shifted to lower AFRs and higher EGR fractions in order to reduce the observed increases in NO_x at the nominal settings, and to more advanced timings in order to mitigate the observed increases in fuel consumption at the nominal settings. These optimal parameter combinations for biodiesel were able to reduce NO_x and noise levels below nominal diesel levels while largely maintaining the substantial PM reductions. These parameter combinations, however, had little (maximum 4% reduction) or no net impact on reducing the biodiesel fuel consumption penalty.     

Explosive Detection by Microthermal Analysis

Differential scanning microcalorimetry at high heating rates of ～ 300°C/s was performed on 30- to 100-µm-size explosive particles using two MEMS-based thermal conductivity gauges in air and under N₂. The gauges consist of a thin-film Si₃N_x membrane with a centrally located Al thin-film heater, which is surrounded by six thin-film Si/Al junctions, creating a temperature-sensitive thermopile (～ 1.3 mV/K) with an effective sensitive area of ca. 200 × 200 µm. Heating was carried out by applying a linear voltage ramp during 1.6 s. The measurements were performed in a specially designed exposure chamber having a transparent glass lid that enabled optical observation of the thermal process.

Besides explosives (TNT, RDX, picric acid, urea nitrate, and TATP) we have studied nonexplosive materials, organic and inorganic, in order to see whether the explosives have a unique response. The materials we studied were oxygen-poor and -rich organic compounds (polyethylene and sugars, respectively), sea sand, and iron flakes.

Clear, well-resolved exotherms were obtained at moderated temperatures (～ 250°C) for all types of explosive materials tested by us. In addition, all explosives exhibited a melting endotherm preceding the exotherm. Sea sand and iron showed no peaks at the heating temperature range. Polyethylene showed an endotherm representing its melting. The sugars showed an endotherm but also an exotherm when heated to elevated temperatures (> 370°C). The thermogram of each material depends on its properties and is characterized by a unique pattern. This pattern may enable the detection and identification of explosive particles using this technology.     

Interface state densities, low frequency noise and electron mobility in surface channel In0.53Ga0.47As n-MOSFETs with a ZrO2 gate dielectric

This paper reports on an investigation of interface state densities, low frequency noise and electron mobility in surface channel In_0.53Ga_0.47As n-MOSFETs with a ZrO₂ gate dielectric. Interface state density values of D_it ∼ 5 × 10¹² cm⁻² eV⁻¹ were extracted using sub-threshold slope analysis and charge pumping technique. The same order of magnitude of trap density was found from low frequency noise measurements. A peak effective electron mobility of 1200 cm²/Vs has been achieved. For these surface channel In_0.53Ga_0.47As n-MOSFETs, it was found that η parameter, an empirical parameter used to calculate the effective electric field, was ∼0.55, and is to be comparable to the standard value found in Si device.     

Number-theoretic functions which are equivalent to number of divisors

Let d(n) denote the number of positive integral divisors of n. In this paper we show that the Möbius function, μ(N), can be computed by a single call to an oracle for d(n). We also show that any function that depends solely on the exponents in the prime factorization of N can be computed by at most log₂ N calls to an oracle for d(N).     

IGC study of surface properties of adsorbed styrene/methacrylic acid diblock polymers

An inverse gas chromatographic study has been carried out on a styrene/methacrylic acid diblock polymer adsorbed on particulates of varying acid/base interaction potential. Acid/base interactions between polymer and substrate were shown to result in the selective adsorption of copolymer moieties, leading to interphase surface compositions that varied with thickness. At higher interphase thicknesses, bulk and surface compositions became similar; however, depending on the interaction potential of the adsorbent, the interphase thicknesses at this point varied from 100 to 1000 Å. Adsorbed diblock interphases are to be considered nonisotropic in local composition and molecular conformation. These properties may make possible designing diblock interphases to meet specific compatibilization requirements in polymer composites. © 1994 John Wiley & Sons, Inc.