Enteropathies with lactose intolerance accompanying chronic otitis media and mastoiditis in infants

The response of the rabbit utero-ovarian ligament to catecholamines has been studied in vitro, with contractile activity being recorded isometrically from whole rabbit ligaments obtained under various hormonal conditions. Both the catecholamines norepinephrine (NE) and epinephrine (EPI) produced dose-dependent (10(-2) mug/ml threshold; 10(2) mug/ml maximum) tetanic contractions of rabbit ligament. There was no difference (P less than 0.05) between estrous and ovulatory ligaments either in terms of the dose-response curves or maximal force of contraction. Both agonists were 100% antagonized by the alpha-blockers, phentolamine and phenoxybenzamine. The beta-agonist, isoproterenol, had little effect on ligament tonus but did inhibit spontaneous activity and attenuated the response to both NE and EPI. Propranolol, a beta-blocker, slightly (less than 10%) potentiated the stimulatory effects of NE and EPI. It is concluded that the utero-ovarian ligament of the rabbit contains predominately alpha-stimulatory and possibly weak beta-inhibitory receptors analogous to receptors in ovarian and oviductal smooth muscles.     

Renal glomerular and tubular lesions in rabbits treated with uranyl nitrate

Renal injury and regeneration was followed light--and electron microscopically in rabbits treated with uranyl nitrate. The presence and importance of glomerular lesions is noteworthy.     

Transplantation of Peyer''s patches under the kidney capsule in syngeneic mice. I. Morphological studies

The morphology of Peyer's patches transplanted under the kidney capsule in syngeneic mice was studied up to 30 days after transplantation. Changes undergoing in transplants were arbitrarily divided into three stages: the initial stage of necrosis, then from the third day on -- clearing of dead tissues, and finally, from the fifth day -- lymphoid regeneration. Starting from the ninth day on, the lymphoid structure of the transplants was quite distinct. Transplants appeared to be similar to Peyer's patches in situ. Reconstituted transplants contained a huge number of blood vessels located with lymphocytes. The epithelial cysts were found in all the transplants. They were formed by remnants of persisted intestinal epithelium covering Peyer's patches in situ. The possibility of epithelial influence on lymphoid regeneration of the transplants is discussed.     

Clinical analysis of lumbar spondylosis

The effect of various heme compounds on growth and maturation of Hymenolepis microstoma in vitro has been determined. At 4 days p.i., worms were collected and cultured in vitro for 6 days in a medium containing various concentrations of haemoglobin, hemin, and bilirubin. Addition of haemoglobin to a medium resulted in significant increases in length, wet weight, number of immature and mature proglottids as compared to the control medium. Supplements of hemin did not seem to affect worm length; however, they caused a significant weight increase, and the numbers of immature and mature proglottids were also more than those in control medium. Bilirubin supplements failed to show any effect on growth and maturation of H. microstoma, and the experimental and control worms were found similar in all respects. In the light of the success with haemoglobin and hemin as growth promoting substances, the role of blood ingredients was also considered. For strobilization and maturation of H. microstoma in vitro, the presence of some kind of heme protein seems essential.     

Failures in the surgical treatment of retinal detachment. An analysis

One hundred sixteen patients underwent operation for renovascular hypertension from 1962 through 1975; 64% had aortorenal reconstruction and 36% had nephrectomy. Sixty-six percent were cured and 19% were improved. Rapid sequence intravenous pyelography, radioisotope renography, and renal arteriography were equal in ability to detect renovascular hypertension. Bilateral renal biopsy specimens had excellent prognostic value when performed in a graded semiquantitative manner. Plasma renin activity was the most consistently useful criterion for prediction of surgical cure if the following requirements were used: (1) elevated peripheral plasma renin activity, (2) elevated renin from the affected kidney, and (3) suppressed renin secretion from the contralateral kidney. An angiotensin II antagonist, saralasin acetate, used in six patients before operation in an attempt to identify those whose hypertension depended on angiotensin II activity, produced a depressor response correlating well with the surgical result.     

The application of NASICON (Na2Zr2Si2PO12) to the thermodynamic study of beta-alumina

The solid-state galvanic cell with NASICON as an auxiliary electrolyte was built. The standard free enthalpy, entropy and enthalpy of beta-alumina formation as well as the Na₂O-activity in the alpha-alumina—beta-alumina coexistence region were determined in the temperature range 970–1220 K. The limiting temperature of spontaneous decomposition of beta-alumina was also estimated. The results were compared with those obtained by other authors.     

Effect of elevated accumulation of iron in ferritin on the antioxidants content in soybean sprouts

Cultivation of soybean sprouts in abiotic stress conditions, resulted from the presence of 5–25?mM FeSO₄ in the culture media, causes a strong overexpression of ferritin. Accumulation of ferritin iron in sprouted seeds germinated in the 20?mM solution of FeSO₄ was 67 times higher than in sprouts germinated in distilled water. The cultivation conditions also influence on another antioxidant content—mainly β-carotene content, which increased 28 times (in sprouts cultured in 10?mM FeSO₄ solution) in comparison to the content in dry seeds. Obtained in stress conditions sprouted seeds contain less tocochromanoles than raw seeds. However, their total tocochromanol content was higher than in sprouted seeds cultured in distilled water in every examined concentration of Fe²⁺. A total antioxidant activity is increased only during culturing in 0–10?mM media, and it is positively correlated to the total phenolic compounds content (r?=?0.8498). We concluded that germination in high abiotic stress also causes the increase in different antioxidants content, not only in ferritin, which is directly involved in the process of iron detoxification.     

Analysis of indole compounds in edible Basidiomycota species after thermal processing

Methanolic extracts of processed fruiting bodies of six edible mushroom species (Basidiomycota) - Armillaria mellea, Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus collected from natural habitats and Pleurotus ostreatus of commercial origin - were analysed for the presence of non-hallucinogenic indole compounds. Thermal processing was designed in such a way that it mimicked conditions used for cooking of mushroom dishes, since only a narrow group of mushrooms can be eaten raw, while indole compounds are thermolabile. All processed extracts were shown to contain l-tryptophan (up to 8.92 mg/100 g dw). The contents of the remaining compounds, 5-methyltryptophan, tryptamine, melatonin, indoleacetonitrile and indole, varied in different species (from 0.71 to 6.55 mg/100 g dw). Extract of processed C. cibarius fruiting bodies contained l-tryptophan, 5-methyltryptophan, tryptamine, melatonin, indoleacetonitrile and indole (1.96-4.94 mg/100 g dw) whereas l-tryptophan (2.78 mg/100 g dw) and tryptamine (2.77 mg/100 g dw) were the only indole compounds identified in the processed fruiting bodies of A. mellea.