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81.
In this study, we aimed to increase the sensitivity of protein labeling using 1.4 nm gold nanoparticles and glutamate δ2 receptor (GluD2) from the postsynaptic membrane of the Purkinje cells. The very small marker size of the particles reduces the steric hindrance between antibodies leading to a higher labeling efficiency of more than one subunit per single receptor molecule. The nanoparticles are visible in 200 kV dark‐field scanning transmission electron microscope on freeze‐fractured carbon replica of nervous tissue after plasma cleaning treatment. The different elemental composition of nanoparticles as Au nanogold or CdS quantum dot can be distinguished by energy dispersive X‐ray spectroscopy. This method ensures detection of an average of three subunits per GluD2 and often labels all four of them with 1.4 nm Au nanoparticles. It is concluded that this high‐resolution microscopic method is useful for exploring the quaternary structure of membrane proteins. Microsc. Res. Tech. 75:1159–1164, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
82.
Clotilde Muller Sandrine Muller Aicha Sissoko Alexandre Klein Bernadette Faller Francois Chantrel 《Hemodialysis international. International Symposium on Home Hemodialysis》2012,16(4):556-558
Lanthanum therapy is an efficient therapy of hyperphosphoremia by chelating phosphore in the digestive tract. Lanthanum is a silvery white metallic element that belongs to group 3 of the periodic table. This drug is lightly absorbed and has low water solubility. It should be borne in mind that abdominal X‐rays of patients taking lanthanum carbonate may have a radio‐opaque appearance typical of imaging agents. This characteristic is suggested to confirm adherence of the patient by doing an abdominal X‐ray. We describe in our case a particular good compliant patient with slow digestive transit, which ends by pseudo‐occlusion. 相似文献
83.
The literature of portfolio optimization is extensive and covers several important aspects of the asset allocation problem. However, previous works consider simplified linear borrowing cost functions that leads to suboptimal allocations. This paper aims at efficiently solving the leveraged portfolio selection problem with a thorough borrowing cost representation comprising a number lenders with different rates and credit limits. We propose a two-stage stochastic programming model for asset and debt allocation considering a CVaR-based risk constraint and a convex piecewise-linear borrowing cost function. We compare our model to its counterpart with the fixed borrowing rate approximation used in literature. Numerical results show our model significantly improves performance in terms of risk-return trade-off. 相似文献
84.
Mohammed Nabil El Korso Rémy Boyer Alexandre Renaux Sylvie Marcos 《Signal processing》2012,92(2):547-552
In this fast communication, we derive the statistical resolution limit (SRL), characterizing the minimal parameter separation, to resolve two closely spaced known near-field sources impinging on a linear array. Toward this goal, we conduct on the first-order Taylor expansion of the observation model a Generalized Likelihood Ratio Test (GLRT) based on a Constrained Maximum Likelihood Estimator (CMLE) of the SRL. More precisely, the minimum separation between two near-field sources, that is detectable for a given probability of false alarm and a given probability of detection, is derived herein. Finally, numerical simulations are done to quantify the impact of the array geometry of the signal sources power distribution and of the array aperture on the statistical resolution limit. 相似文献
85.
Natalia D. Lenenko Elena N. Semina Vladimir I. Zaikovskii Alexandre S. Golub 《Materials Chemistry and Physics》2013
Microstructure of layered nanocomposite compound consisting of molybdenum disulfide single layers and the layers of octadecyltrimethylammonium molecules as well as the structure of destruction products of this hybrid compound were studied by high resolution transmission electron microscopy (HRTEM) and powder X-ray diffraction (XRD) techniques. Changes in composition, ordering and morphology of the host and guest layers of the compound occurring due to release of organic guest from the interlayer space of MoS2 on heating or on action of electronic beam have been revealed. Removal of the guest was found to initiate formation in the initial layered structure of the packets consisting of a few MoS2 layers which come close together within the distances of ca. 1–1.5 nm and 0.6–0.7 nm after heating at 250 °C and 400 °C, respectively. Leaving the guest also causes deformations of MoS2 layers resulting in their non-flat geometry. At 400 °C, strong bending of a part of the sulfide layers with the radius as small as 3–4 nm was observed. 相似文献
86.
Norberto Labrador Delia Gutiérre-Campos Olivier Rapaud Hélène Ageorges Alexandre Maitre 《Ceramics International》2019,45(9):11677-11685
High-purity nanocrystalline aluminum nitride powders were synthesized by using a 12?kW non-transferred arc plasma. The synthesis was conducted in a versatile, new designed, one-chamber thermal plasma reactor (TPR). The novel experimental assembly incorporated better working conditions like: high temperature gradient between the crucible and reactor's wall, and high super-saturation of the system by nitrogen and carbon. Thermodynamic modelling of the synthesis was conducted in order to achieve the best conditions for AlN formation. In this study, aluminum discs of Al 1100 were used as precursor material and pure nitrogen was the only gas used as reagent and plasmogenic gas.Nanopowders collected from reactor's wall were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-Ray diffraction (XRD). Synthesized h-AlN nano-powders were found to be free of oxides and aluminum metal. A thin carbon-layer around the particles was detected. TEM results indicated that the carbon-layer was around 5 and 10?nm. This outcome could make a significant difference with other synthesis reported in the literature since the occurrence of the carbon-layer, could delay AlN oxidation, prevent hydration, and could avoid the agglomeration of the particles. 相似文献
87.
Lucas Alves Da Silva Eric Dekneuvel Alexandre Lewicki Benjamin Nicolle Gilles Jacquemod 《Microelectronics Journal》2012,43(2):98-109
In this paper, we propose to develop a hierarchical library associated with various simulators that can be used in a single platform, called TrustMe-ViP, which enables a unique simulation framework and full model interoperability. Such platform is dedicated to complex SoC design, such as trusted personal devices where cost and time-to-market are very important constraints. To validate this methodology, we present the estimation of BER and power consumption for a Bluetooth transceiver. 相似文献
88.
The problem of Information Technology energy consumption has gained much attention due to the always increasing use of IT both for business and for personal reasons. In particular, data centers are now playing a much more important role in the modern society, where the information is available all the time and everywhere. In this context, the aim of this paper is to study energy efficiency issues within data centers from the Information System perspective. The proposed approach integrates the application and infrastructure capabilities, in which the enactment of adaptation mechanisms is aligned with the business process. Based on both energy and quality dimensions of service-based applications, a model-based approach supports the formulation of new constrained optimization problem that takes into consideration over-constrained solutions where the goal is to obtain the better trade-off between energy and quality requirements. These ideas are combined within a framework where time-based analysis allow the identification of potential system threats and drive the selection of adaptation actions improving overall energy and quality requirements, represented by indicators satisfaction. In addition, the framework includes an evolution mechanism that is able to evaluate past decisions feedback in order to adjust the model according to the current underlying environment. Finally, the benefits of the approach are analyzed in an experimental setting. 相似文献
89.
90.
Valentina Paracchini Mauro Petrillo Antoon Lievens Dafni-Maria Kagkli Alexandre Angers-Loustau 《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2019,36(1):1-14
Gadoids are a group of fish with historical importance in the fishing industry. The high demand for cod is one of the reasons why cod products are often mislabelled, and numerous observations have been made on the replacement of Atlantic cod (Gadus morhua) by cheaper species or its illegal capture in contravention of fish quotas. Fish species identification is traditionally based on morphological features, but this may be difficult in case of heat-treated or processed products, or where the species look similar, as in the Gadoid group. DNA-based approaches (using either nuclear or mitochondrial DNA) are most commonly used in this case, due to their high specificity and to the high resilience of the target molecules to food processing techniques. In this article, we identified, using an automated screening approach, novel barcode regions and their associated primers in the nuclear genome, to be used for the efficient identification of Gadoids. The barcode regions were tested on official and commercial samples, raw or mildly treated products, like frozen, or salted, as well as pre-cooked complex mixtures and processed samples, using next-generation sequencing (NGS) technique. The method proposed could complement existing fish identification strategies in establishing an efficient framework to detect and prevent frauds along the food chain. 相似文献