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101.
Shela Gorinstein Jerzy Drzewiecki Efren Delgado-Licon Elke Pawelzik Alma Leticia Martinez Ayala Oscar J. Medina Ratiporn Haruenkit Simon Trakhtenberg 《European Food Research and Technology》2005,221(1-2):69-77
Comparative protein studies of cereals and pseudocereals are important, especially in cases of cereal protein allergy when pseudocereal substitution is unavoidable. Therefore, ten species and cultivars belonging to different Angiosperms families (Oryza sativa normal Poaceae, bran Poaceae and Jasmin Hom Dokmali Poaceae, Sorghum technicum Battand et Traubt Poaceae, Glycine max L. Merr. Fabaceae, Fagopyrum esculentum Mnch. Polygonaceae, Chenopodium quinoa Wild Chenopodiaceae, Amaranthus hybridum v.1004 Amaranthaceae, cruentus v. R104 Amaranthaceae and hypochondriacus v.1023 Amaranthaceae) were examined by sodium dodecyl sulphate (SDS-PAGE) seed protein markers, fluorescence, circular dichroism (CD) spectra and Fourier transform infrared (FT-IR) measurements. A high degree of polymorphism of all species and cultivars was found. Amaranth species have very similar seed protein electrophoretic profiles. According to UPGMA algorithm the examined species and varieties could be clustered into two similarity groups. Soybean, quinoa, buckwheat and Amaranth (as a genus) can be considered as phylogenic distant taxa. The fluorescence properties of amaranth, soybean, quinoa, rice and buckwheat soluble protein fractions were measured by fluorescence of tryptophan at 295 nm, light intensity, peak response and shift in the maximum of emission. Relative structural stabilities of native proteins were estimated by CD and FTIR. Similarities were found between these plants, which could make them a substitution of each other as well as for cereals.Author, S. Gorinstein is affiliated with the David R. Bloom Center for Pharmacy. 相似文献
102.
Berlanga-Reyes CM Carvajal-Millan E Lizardi-Mendoza J Islas-Rubio AR Rascón-Chu A 《International journal of molecular sciences》2011,12(9):5853-5861
Ferulated arabinoxylans were alkali-extracted from wheat bran at different incubation times (0.0, 0.5, 1.0, 1.5 and 2.0 h). Wheat bran ferulated arabinoxylans (WBAX) arabinose-to-xylose ratio, ferulic acid content, intrinsic viscosity and viscosimetric molecular weight values decreased as the incubation time of extraction increased. WBAX enzymatic cross-linking capability was affected by incubation time while an increase in WBAX concentration from 5 to 6% (w/v) favored gelation. The WBAX gels formed presented a macroporous structure with mesh size ranging from 40 to 119 nm and hardness values varying from 1.7 to 5 N. 相似文献
103.
Four expanded granular sludge bed (EGSB) bioreactors were seeded with a mesophilically-grown granular sludge and operated in duplicate for mesophilic (37 °C; R1 & R2) and low- (15°; R3 & R4) temperature treatment of a synthetic volatile fatty acid (VFA) based wastewater (3 kg COD m−3 d−1) with one of each pair (R1 & R3) supplemented with increasing concentrations of trichloroethylene (TCE; 10, 20, 40, 60 mg l−1) and one acting as a control. Bioreactor performance was evaluated by % COD removal efficiency and % biogas methane (CH4) content. Quantitative Polymerase Chain Reaction (qPCR) was used to investigate the methanogenic community composition and dynamics in the bioreactors during the trial, while specific methanogenic activity (SMA) and toxicity assays were utilized to investigate the activity and TCE/dichloroethylene (DCE) toxicity thresholds of key trophic groups, respectively. At both 37 °C and 15 °C, TCE levels of 60 mg l−1 resulted in the decline of % COD removal efficiencies to 29% (Day 235) and 37% (Day 238), respectively, and in % biogas CH4 to 54% (Day 235) and 5% (Day 238), respectively. Despite the inhibitory effect of TCE on the anaerobic digestion process, the main drivers influencing methanogenic community development, as determined by qPCR and Non-metric multidimensional scaling analysis, were (i) wastewater composition and (ii) operating temperature. At the apical TCE concentration both SMA and qPCR of methanogenic archaea suggested that acetoclastic methanogens were somewhat inhibited by the presence of TCE and/or its degradation derivatives, while competition by dechlorinating organisms may have limited the availability of H2 for hydrogenotrophic methanogenesis. In addition, there appeared to be an inverse correlation between SMA levels and TCE tolerance, a finding that was supported by the analysis of the inhibitory effect of TCE on two additional biomass sources. The results indicate that low-temperature anaerobic digestion is a feasible approach for the treatment of TCE-containing wastewater. 相似文献
104.
Shela Gorinstein Oscar J. Medina Vargas Nicolas O. Jaramillo Ines Arnao Salas Alma Leticia Martinez Ayala Patricia Arancibia-Avila Fernando Toledo Elena Katrich Simon Trakhtenberg 《European Food Research and Technology》2007,225(3-4):321-328
The objective of the present study was to investigate the effect of phenolic substances and proteins on the antioxidant potentials
in some cereals and pseudocereals and to compare their bioability. The polyphenol dry matter extracts (PDME) from the investigated
seeds of buckwheat, rice, soybean, amaranth and quinoa with 1.2 M HCl in 50% methanol/water (PDME50%Met/HCl) exhibited higher
inhibition of lipid peroxidation than the ones extracted with 50% methanol/water (PDME50%Met) and were comparable to the antioxidant
activity of butylated hydroxyanisole at concentration of 0.2 mg mL−1. The antioxidant activities of these seed extracts determined by 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonate)-ABTS●+/K2S2O8, β-carotene bleaching (β-carotene), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging methods showed high correlation
coefficients (R
2) such as 0.9515, 0.9058 and 0.8723, respectively, with the presence of total polyphenols estimated by Folin-Ciocalteu assay.
These results indicate that the major antioxidant components in these extracts mostly derived from the polyphenols, and proteins
showed only minimal values of bioactivity. Based on high contents of polyphenols, anthocyanins, flavonoids and their antioxidant
activities pseudocereals such as buckwheat, quinoa and amaranth can be a substitute for cereals for common and atherosclerotic
diets and sometimes in the allergic cases. 相似文献
105.
René Köppel Alma Sendic Hans-Ulrich Waiblinger 《European Food Research and Technology》2014,239(4):653-659
According to the EU and Swiss food legislation, only deregulated traits of transgenic plants are allowed to be imported and sold to the consumer. In order to control imports of soya and maize products from retailers, efficient and reliable methods for the detection and quantification are a prerequisite. The screening for specific DNA elements characteristic of transgenic plants is crucial for further analysis and has a major impact on the efficiency of the whole analysis workflow. To allow laboratories to efficiently and reliably screen food products for transgenic plant products, two novel multiplex real-time polymerase chain reaction (PCR) systems were developed and validated. One system determines DNA contents from maize, soya, cauliflower mosaic virus (CaMV) 35S promoter (P35S), NOS terminator from Agrobacterium tumefaciens and CaMV, and the second PCR system simultaneously detects DNA sequences from figwort mosaic virus promoter (PFMV), from bar gene of Streptomyces hygroscopicus, from gene coding for phosphinothricin acetyltransferase (PAT) and from a DNA construct of enolpyruvyl shikimate phosphate synthase gene (CP4-EPSPS) and Arabidopsis thaliana (CPT2). The tests exhibit good specificity and a limit of detection of at least 0.1 % for all analytes. 相似文献
106.
Ali Temiz Gaye Kose Dmitri Panov Nasko Terziev Mehmet Hakkı Alma Sabrina Palanti Selcuk Akbas 《应用聚合物科学杂志》2013,130(3):1562-1569
In this article, the effects of bio‐oil and epoxidized linseed oil (ELO) on water absorption, tangential swelling, decay and insect resistance, thermo‐gravimetric analysis, and mechanical properties of treated wood samples were studied. The bio‐oil used in this article was by‐product of ThermoWood thermal modification process. Linseed oil and hydrogen peroxide were used to prepare ELO. The results indicated that the samples treated with bio‐oil had lower water absorption than that of the control group. The second treatment with ELO significantly reduced further the water absorption. The decay resistance of treated wood samples with 20% of bio‐oil against brown (Coniophora puteana) and white rot (Trametes versicolor) fungi was very high. According to the insect test results, increasing bio‐oil concentration from 10% to 20% significantly decreased surviving rate of Hylotrupes bajulus. Thermo‐gravimetric analysis showed that all treated samples had higher initial deterioration temperature than that of the control group. Regarding the wood strength, the impregnated bio‐oil generally reduced the mechanical properties of wood except modulus of elasticity (MOE). © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 1562–1569, 2013 相似文献
107.
108.
Alexander N. Bondarchuk Alexander B. Glot Alma R. Velasco-Rosales 《Ceramics International》2018,44(7):7844-7850
It is shown that an addition of Sb2O5 or Nb2O5 (0.05–0.15?mol%) to the system SnO2–CoO–Cr2O3–Bi2O3 leads to the enhancement of grain growth. This effect is associated with the presence of the liquid Bi-phase in ceramics during sintering. The obtained ceramics possess non-linear current-voltage characteristics and can be used for preparing low voltage varistors. The non-linearity coefficient α reaches 22 and the characteristic electric field 692?V/cm for Nb-doped materials and 11 and 421?V/cm respectively for Sb-doped ceramics materials. The results of dc and ac electrical measurements, as well as scanning electron microscopy are presented and discussed in terms of the known barrier model for varistors. 相似文献
109.
Mathieu Lepot Jean-Baptiste Aubin François H. L. R. Clemens Alma Mašić 《Urban Water Journal》2017,14(9):908-921
UV/Vis spectrophotometers have been used to monitor water quality since the early 2000s. Calibration of these devices requires sampling campaigns to elaborate relations between recorded spectra and measured concentrations. In order to build robust calibration data sets, several spectra must be recorded per sample. This study compares two approaches – principal component analysis and data depth theory – to identify outliers and select the most representative spectrum (MRS) among the repetitively recorded spectra. Detection of samples that contain outliers is consistent between the methods in more than 70% of the samples. Identification of spectra as outliers is consistent in more than 95% of the cases. The identification of MRS differs depending on the approach used. In their current form, both of the proposed approaches can be used for outlier detection and identification. Further studies are suggested to combine the methods and develop an automated ranking and sorting system. 相似文献
110.
Administered O. J. Harvey's "This I Believe" Test to undergraduates. 55 Ss were selected with concrete belief systems and 29 with abstract systems. Ss then played the role of arguing against their own stand on an ego-involving issue which was scored for 5 attributes: number to topics discussed, number of approaches utilized in the arguments, openness to negative evaluation, ethnocentrism, and absolutism. Ss also completed the WAIS Digit-Symbol Substitution test, a vocabulary test, an arithmetic test, the A-State scale of the State-Trait Anxiety Inventory. On the basis of the anxiety measure, Ss were divided into "high" and "low" anxiety groups, and a 2-way analysis of variance (Concreteness-Abstractness * Anxiety) was run for each of the 8 dependent variables. Anxiety exercised a significant main effect on none of the 8 dependent variables, while concreteness-abstractness significantly affected 5. The interaction of these 2 variables, while in the anticipated direction in all instances, significantly affected performance only on the vocabulary measure. (29 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献