Guarding galleries and terrains

Let P be a polygon with n vertices. We say that two points of P see each other if the line segment connecting them lies inside (the closure of) P. In this paper we present efficient approximation algorithms for finding the smallest set G of points of P so that each point of P is seen by at least one point of G, and the points of G are constrained to be belong to the set of vertices of an arbitrarily dense grind. We also present similar algorithms for terrains and polygons with holes.     

Note: A scanning electron microscope sample holder for bidirectional characterization of atomic force microscope probe tips

A novel sample holder that enables atomic force microscopy (AFM) tips to be mounted inside a scanning electron microscopy (SEM) for the purpose of characterizing the AFM tips is described. The holder provides quick and easy handling of tips by using a spring clip to hold them in place. The holder can accommodate two tips simultaneously in two perpendicular orientations, allowing both top and side view imaging of the tips by the SEM.     

Can art breach boundaries? Segregation and hierarchy at a fringe theatre festival in the Israeli mixed city of Acre

Abstract

This study explores the relationship between art and urban boundaries using the case study of a fringe theatre festival in the Israeli mixed-city of Acre. While mixed cities today are understood as agglomerations of enclaves, maintained and reinforced by boundaries, urban designers and artists have used art as a culture-led regeneration strategy through which these boundaries may be breached. This study undermines the shared assumption of both fields: that art has the power to breach boundaries, by juxtaposing a city’s artistic activity with its segregation patterns and boundaries. Using super-positioning, the findings of two research methods have been integrated: urban research and ethnographic field work. The article shows that although the artistic activity in question is rooted in an avant-garde radical desire to subvert socioeconomic structures, it actually produces new versions and interpretations of the same segregations and boundaries in both space and society.     

“Weighting for more”: Enhancing characteristic-function based ICA with asymptotically optimal weighting

The CHaracteristic-function-Enabled Source Separation (CHESS) method for independent component analysis (ICA) is based on approximate joint diagonalization (AJD) of Hessians of the observations' empirical log-characteristic-function, taken at selected off-origin “processing points”. As previously observed in other contexts, the AJD performance can be significantly improved by optimal weighting, using the inverse of the covariance matrix of all of the off-diagonal terms of the target-matrices. Fortunately, this apparently cumbersome weighting scheme takes a convenient form under the assumption that the mixture is already “nearly separated”, e.g., following some initial separation. We derive covariance expressions for the Sample-Hessian matrices, and show that under the near-separation assumption, the weight matrix takes a nearly block-diagonal form, conveniently exploited by the recently proposed WEighted Diagonalization using Gauss itErations (WEDGE) algorithm for weighted AJD. Using our expressions, the weight matrix can be estimated directly from the data—leading to our WeIghTed CHESS (WITCHESS) algorithm. Simulation results demonstrate how WITCHESS can lead to significant performance improvement, not only over unweighted CHESS, but also over other ICA methods.     

Gene Variant of Barrier to Autointegration Factor 2 (Banf2w) Is Concordant with Female Determination in Cichlids

Oreochromis fishes exhibit variability of sex-determination (SD) genes whose characterization contributes to understanding of the sex differentiation network, and to effective tilapia farming, which requires all-male culture. However, O. niloticus (On) amh is the only master-key regulator (MKR) of SD that has been mapped (XY/XX SD-system on LG23). In O. aureus (Oa), LG3 controls a WZ/ZZ SD-system that has recently been delimited to 9.2 Mbp, with an embedded interval rich with female-specific variation, harboring two paics genes and banf2. Developing genetic markers within this interval and using a hybrid Oa stock that demonstrates no recombination repression in LG3, we mapped the critical SD region to 235 Kbp on the orthologous On physical map (p < 1.5 × 10⁻²⁶). DNA-seq assembly and peak-proportion analysis of variation based on Sanger chromatograms allowed the characterization of copy-number variation (CNV) of banf2. Oa males had three exons capable of encoding 90-amino-acid polypeptides, yet in Oa females, we found an extra copy with an 89-amino-acid polypeptide and three non-conservative amino acid substitutions, designated as banf2w. CNV analysis suggested the existence of two to five copies of banf2 in diploidic Cichlidae. Disrupting the Hardy–Weinberg equilibrium (p < 4.2 × 10⁻³), banf2w was concordant with female determination in Oa and in three cichlids with LG3 WZ/ZZ SD-systems (O. tanganicae, O. hornorum and Pelmatolapia mariae). Furthermore, exclusive RNA-seq expression in Oa females strengthened the candidacy of banf2w as the long-sought LG3 SD MKR. As banf genes mediate nuclear assembly, chromatin organization, gene expression and gonad development, banf2w may play a fundamental role inducing female nucleus formation that is essential for WZ/ZZ SD.     

Differential Effects of Halofuginone Enantiomers on Muscle Fibrosis and Histopathology in Duchenne Muscular Dystrophy

Progressive loss of muscle and muscle function is associated with significant fibrosis in Duchenne muscular dystrophy (DMD) patients. Halofuginone, an analog of febrifugine, prevents fibrosis in various animal models, including those of muscular dystrophies. Effects of (+)/(−)-halofuginone enantiomers on motor coordination and diaphragm histopathology in mdx mice, the mouse model for DMD, were examined. Four-week-old male mice were treated with racemic halofuginone, or its separate enantiomers, for 10 weeks. Controls were treated with saline. Racemic halofuginone-treated mice demonstrated better motor coordination and balance than controls. However, (+)-halofuginone surpassed the racemic form’s effect. No effect was observed for (−)-halofuginone, which behaved like the control. A significant reduction in collagen content and degenerative areas, and an increase in utrophin levels were observed in diaphragms of mice treated with racemic halofuginone. Again, (+)-halofuginone was more effective than the racemic form, whereas (−)-halofuginone had no effect. Both racemic and (+)-halofuginone increased diaphragm myofiber diameters, with no effect for (−)-halofuginone. No effects were observed for any of the compounds tested in an in-vitro cell viability assay. These results, demonstrating a differential effect of the halofuginone enantiomers and superiority of (+)-halofuginone, are of great importance for future use of (+)-halofuginone as a DMD antifibrotic therapy.     

Obtaining small photovoltaic array operational curves for arbitrary cell temperatures and solar irradiation densities from standard conditions data

The paper presents a simple approach to deriving I–V curves of photovoltaic panels and small arrays for arbitrary environmental conditions on the basis of three points of a single operating curve data and short current temperature coefficient only. The proposed method does not employ fitting of any type and is solely based on a numerical solution of a system of transcendental equations. The equations are expressed in a dimensionless form, simplifying both the solution and photovoltaic panel parameters' representation. The solution is used to find the values of normalized equivalent circuit elements for the available data and then perform an appropriate adjustment to obtain the operating curves for arbitrary conditions. The proposed method was applied to monocrystalline and polycrystalline commercial solar panels and was compared with both manufacturer‐provided and experimentally measured operating curves to analyze the approach applicability and accuracy. Copyright © 2012 John Wiley & Sons, Ltd.