Interactions of lactone, carboxylate and self-aggregated forms of camptothecin with human and bovine serum albumins

Highly degenerate primers to conserved regions of the eukaryotic phosphoinositol-specific phospholipase C (PLC) were used to amplify fragments of plant PLCs from Arabidopsis thaliana genomic DNA. Eight completely different fragment sequences that showed high homology to PLCs of both animals and plants were isolated. The variation between these putative PLCs was high and suggests that, like animals, plants have multiple isoforms of PLC. Using one of the PCR clones, we isolated a corresponding full-length Arabidopsis PLC gene (ATHATPLC1G), and sequence analysis indicated that it was most like a delta-type PLC. This gene is 2.5 kb and contains seven introns, all but one of which has intron/exon border sequences that conform to the Arabidopsis consensus. The structural complexity of the gene is relatively simple compared to mammalian beta-type PLCs that can be 15 kb long with up to 30 introns. The plant gene is a single copy and was mapped to four Arabidopsis YACs, one located on chromosome 2. The promoter region contained two TATA-like elements at -43 and -185 and other putative regulatory elements that suggest that this PLC is hormonally regulated. This is the first plant PLC gene and the first delta type-PLC gene from a higher organism to be sequenced.     

Coronary bypass in vascular patients: a relatively high-risk procedure

A premise of cardiac risk stratification is that the added risk of coronary artery bypass grafting (CABG) is offset by the improved safety of subsequent vascular reconstruction (VR). We questioned if elective CABG is patients with severe peripheral vascular disease (PVD) is a relatively high-risk procedure. A cohort study of 680 elective CABG patients from January 1993 to December 1994 was performed using three mutually exclusive outcomes of complication-free survival, morbidity, and mortality. Patient characteristic, operative, and outcome data were prospectively collected. Retrospective review determined that 58 patients had either a standard indication for or a history of VR. Overall CABG mortality was 2.5%, with statistically similar but relatively higher rates for PVD as compared to non-PVD patients. In contrast, major morbidity occurred at rates 3.6-fold higher in PVD patients (39.7%) than in disease-free patients (16.7%) after adjustment for the effects of patient and operative variables (odds ratio [OR] 3.67, 95% confidence interval [CI] 1.93-6.99). CABG morbidity in the PVD patient was most likely in those patients with aortoiliac (OR 9.51, CI 3.20-28.27) and aortic aneurysmal (OR 5.24, CI 1.28-21.41) disease types. CABG in PVD patients is associated with significant major morbidity. Such morbidity may preclude or alter the timing of subsequent VR.     

Sort vs. hash revisited

Efficient algorithms for processing large volumes of data are very important both for relational and new object-oriented database systems. Many query-processing operations can be implemented using sort- or hash-based algorithms, e.g. intersections, joins, and duplicate elimination. In the early relational database systems, only sort-based algorithms were employed. In the last decade, hash-based algorithms have gained acceptance and popularity, and are often considered generally superior to sort-based algorithms such as merge-join. In this article, we compare the concepts behind sort- and hash-based query-processing algorithms and conclude that (1) many dualities exist between the two types of algorithms, (2) their costs differ mostly by percentages rather than by factors, (3) several special cases exist that favor one or the other choice, and (4) there is a strong reason why both hash- and sort-based algorithms should be available in a query-processing system. Our conclusions are supported by experiments performed using the Volcano query execution engine     

Parallel architecture for fast transforms with trigonometric kernel

We present an unified parallel architecture for four of the most important fast orthogonal transforms with trigonometric kernel: Complex Valued Fourier (CFFT), Real Valued Fourier (RFFT), Hartley (FHT), and Cosine (FCT). Out of these, only the CFFT has a data flow coinciding with the one generated by the successive doubling method, which can be transformed on a constant geometry flow using perfect unshuffle or shuffle permutations. The other three require some type of hardware modification to guarantee the constant geometry of the successive doubling method. We have defined a generalized processing section (PS), based on a circular CORDIC rotator, for the four transforms. This PS section permits the evaluation of the CFFT and FCT transforms in n data recirculations and the RFFT and FHT transforms in n-1 data recirculations, with n being the number of stages of a transform of length N=rⁿ. Also, the efficiency of the partitioned parallel architecture is optimum because there is no cycle loss in the systolic computation of all the butterflies for each of the four transforms     

The classification, fusion, and parallelization of array languageprimitives

We present a classification scheme for array language primitives that quantifies the variation in parallelism and data locality that results from the fusion of any two primitives. We also present an algorithm based on this scheme that efficiently determines when it is beneficial to fuse any two primitives. Experimental results show that five LINPACK routines report 50% performance improvement from the fusion of array operators     

A closer look at mutation in genetic algorithms

This paper examines the influence of mutation on the behavior of genetic algorithms through a series of examples and experiments. The results provide an existence proof that mutation is a far more profound operator than has ever been recognized. Implications are discussed which point to the importance of open questions concerning genetic algorithms. The paper also reviews the implementation of the infinite population model of Vose which forms the computational basis of this investigation.     

Structuring fault-tolerant object systems for modularity in adistributed environment

The object-oriented approach to system structuring has found widespread acceptance among designers and developers of robust computing systems. The authors propose a system structure for distributed programming systems that support persistent objects and describe how properties such as persistence and recoverability can be implemented. The proposed structure is modular, permitting easy exploitation of any distributed computing facilities provided by the underlying system. An existing system constructed according to the principles espoused here is examined to illustrate the practical utility of the proposed approach to system structuring