Association between dietary patterns and metabolic syndrome in a sample of portuguese adults

ABSTRACT: BACKGROUND: There is scarce evidence regarding the association between diet and metabolic syndrome (MetS) in Portuguese population. We aim to evaluate the association between a posteriori dietary patterns (DPs) and MetS and its features. METHODS: Using random digit dialing, a sample of 2167 adults was selected between 1999 and 2003, in Porto. During a face-to-face interview, a questionnaire was applied, anthropometric measures were taken, blood pressure measured and a fasting blood sample collected. Diet was assessed using a validated food frequency questionnaire, and four DPs were identified in each sex by multivariate finite mixture models. RESULTS: After adjustment for age and daily energy intake, comparing to the "healthy" DP, women with the "low fruit and vegetables" DP had a higher odds of high waist circumference (OR = 1.88 95% CI 1.17-3.01) and low HDL-cholesterol (OR = 1.78 95% IC 1.12-2.82) and women in the "red meat and alcohol" DP had higher odds of high waist circumference (OR = 1.45 95% CI 1.01-2.07) and of MetS (OR = 1.57 95% CI 1.07-2.29); men with the "fish" DP had a higher odds of high triglycerides (OR = 1.57 95% CI 1.05-2.35). After further adjustments (education, physical activity, smoking, alcohol drinking, BMI, and menopausal status) no significant associations remained. CONCLUSIONS: Four distinct DPs were identified in a community sample of Portuguese adults and there was no association with the prevalence of MetS.     

Synergistic effects between κ-carrageenan and locust bean gum on physicochemical properties of edible films made thereof

The development of mixed systems, formed by locust bean gum (LBG), and κ-carrageenan (κ-car) can offer new interesting applications such as the development of edible films with particular properties. κ-car/LBG blend films with different ratios were developed, and their effects on films’ physical properties were assessed. Thermogravimetric analysis (TGA), X-ray diffraction (XRD) patterns, dynamic mechanical analysis (DMA) and Fourier-transform infrared (FTIR) spectroscopy techniques were used to highlight the interactions between the two polysaccharides. The addition of κ-car to LBG improved the barrier properties of the films leading to a decrease of water vapor permeability (WVP). Improved values of elongation-at-break (EB) were registered when the ratio of κ-car/LBG was 80/20 or 40/60 (% w/w). Moreover, the κ-car/LBG blend films enhance the tensile strength (TS) compared to κ-car and LBG films. FTIR results suggested that hydrogen bonds interactions between κ-car and LBG have a great influence in films’ properties e.g. moisture content, WVP. Therefore, different κ-car/LBG ratios can be used to tailor edible films with enhanced barrier and mechanical properties.     

RNA fingerprinting analysis of Oenococcus oeni strains under wine conditions

Oenococcus oeni is a lactic acid bacterium of economic interest used in winemaking. This bacterium is the preferred species for malolactic fermentation (MLF) due its adaptability to the chemically harsh wine environment. MLF enhances the organoleptic properties and ensures deacidification of wines.     

Rheology and thermal behavior of polyamide reinforced with alumina whiskers

Alumina whiskers were melt‐mixed with polyamide 12 to obtain new composites with high performance properties and similar processability of neat polyamide. Three kinds of alumina whiskers were used to prepare composites (purified fibers, ribbon‐shaped fibers and silica‐coated fibers). The rheology, morphology and thermal behavior of composites were evaluated as function of the type of whisker and its amount in the samples. In terms of rheological properties, modulus and viscosity were enhanced as function of the amount of filler added. Good dispersion of alumina whiskers in PA12 and an optimal filler‐matrix interface were observed in morphological tests independently of the kind of fiber used. Studies on crystallization behavior indicated the nucleation action of whiskers, although the most important changes in crystalline state of PA12 were observed with ribbon‐shaped and silica‐coated fibers. Whiskers improved significantly the thermal stability of polyamide. But kinetics of degradation process studied using Coats‐Redfern method indicates that the best behavior wasobtained principally with ribbon‐shaped and silica‐coated whiskers. POLYM. COMPOS., 2012. © 2012 Society of Plastics Engineers     

A multi-residue method for the determination of 90 pesticides in matrices with a high water content by LC-MS/MS without clean-up

A method using QuEChERS extraction and LC-MS/MS in electrospray positive ionisation mode was developed and validated for the analysis of 90 pesticides in a high water content matrix (tomato) in a single chromatographic run. To assess the intra-laboratory reproducibility of the method, validation was conducted on four different days by two different analysts. The validation data was treated using a spreadsheet developed in-house, which sets the most appropriate model for linear fit by determining whether the residuals of the calibration curves are homocedastic or heterocedastic. A statistical test for the significance of regression was also carried out. Calibration was always matrix-matched and the curves were obtained over the range 0.0075-0.10 or 0.020-0.125 mg kg(-1). Identification of analytes was based on retention times and MRM ratios. Recoveries were assessed at four different levels for each analyte and were between 73 and 106%, with relative standard deviations under reproducibility conditions of <20%. The measurement uncertainties of the method for each pesticide analysed were below 50%. Previous validation of the same method, applied to papaya samples and satisfactory results obtained in various proficiency tests with different high water content matrices, demonstrated the applicability of the method to these classes of commodities, without clean-up. The validated method will be applied routinely in the pesticide residues monitoring programme that constitutes the National Residue and Contaminant Control Plan of Brazil.     

Technological Performance of the Enterocin A Producer <Emphasis Type="Italic">Enterococcus faecium</Emphasis> MMRA as a Protective Adjunct Culture to Enhance Hygienic and Sensory Attributes of Traditional Fermented Milk ‘Rayeb’

Enterococcus faecium MMRA is an enterocin A producer isolated from ‘Rayeb’, a Tunisian fermented milk drink. In this work, safety aspects and its behaviour in raw milk were investigated to assess its suitability as a protective adjunct culture. E. faecium MMRA showed interesting features such as the absence of several virulence traits, susceptibility to vancomycin and other clinically relevant antibiotics, and lack of haemolytic activity. To evaluate its performance as an adjunct culture for Rayeb, changes in the overall composition of control (non-inoculated) and experimental Rayeb (inoculated with 1%, v/v, E. faecium MMRA) were determined throughout duplicate fermentations of raw milk using microbiological, chemical, HPLC and headspace GC-MS analyses. E. faecium MMRA could multiply in raw milk and produced enterocin A. Interestingly, a higher content of volatile compounds including ethanol, diacetyl and 2-propanol was observed in the presence of this bacteriocin producer. Furthermore, this strain was capable of inhibiting the growth of Listeria monocytogenes CECT 4032 in pasteurised milk, although total killing was not achieved. Further experiments confirmed the development of resistant variants to enterocin A. On similar challenge assays, L. monocytogenes CECT 5672 growth was halted by the presence of the enterocin producer, but viability was only slightly reduced during cold storage. According to our results, E. faecium MMRA meets the criteria for an autochthonous protective adjunct culture to enhance both the hygienic and the sensory attributes of Rayeb.