Adjuvant arthritis: influence of the adjuvant volume and composition on the non-specific inflammation

Adjuvant-induced arthritis is an animal model of chronic inflammatory disease widely used in anti-inflammatory and immunosuppressive drugs testing. When the development and the inhibition of the induced arthritis are measured by the injected paw oedema, it is difficult to delineate the immunological contribution from the persistent non-specific primary section. To study the influence of volume and composition of the injected adjuvant upon the primary non-specific inflammation, we devised a 3X4 factorial experiment on a strain of inbred rats with a low susceptibility to adjuvant-induced arthritis. The injection of mineral oil alone produces a persistent oedema. The injection of mycobacteriae in suspension in saline induces a rapid inflammatory response followed by a fast decrease of the oedema. When complete adjuvant is used, there is always a very strong interaction between the effects of the two components of the adjuvant, i.e. the measured oedemas are much greater than the calculated values, For a given injected volume, the inflammation is maximum when the concentration of mycobacteriae is 2.5 mg/ml. All the rats injected with complete adjuvant present a transient oedema of the non-injected hind paw. This oedema is very small and proportional to the amount of mycobacteria injected.     

Agricola und die Geologie

This paper on the occasion of the 500th birthday of the great Saxon humanistic scholar Georgius Agricola deals with his contributions to geology in the narrow sense. These, contrary to his voluminous works on mining, metallurgy and mineralogy (De re metallica and De natura fossilium, resp.) are parts of the booksDe ortu et causis subterraneorum andDe natura eorum, quae effluunt ex terra. In those we find statements on the causes of earthquakes, on volcanos and the subterranean fire as well as the effects of the running water in mountain-building. Agricola's ideas on geological time and his statements on dynamics of the Earth's crust are remarkable.     

The multicultural perspective in therapy: A social constructionist approach.

Discusses the theoretical and clinical applications of the postmodern paradigm of social constructionism as a basis for counseling culturally different clients. Such applications avoid the trap of perpetuating the status-quo when working with minority and marginalized clientele. The main points of multiculturalism and social constructionism are summarized, and issues of mutual concern to both perspectives are examined. Applications to psychotherapy are explored, and the challenges of applying the social constructionist approach to culturally competent counseling are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Isolation and characterization of three Dictyostelium myosin-I isozymes

Using ion exchange chromatography and an ATP-dependent actin precipitation step, we have isolated three myosin-I isozymes that, together, account for most of the K+EDTA-ATPase activity recovered from extracts of Dictyostelium. The two major myosin-I isozymes, present in approximately equal amounts, had apparent molecular masses of 125 kDa on SDS gels and have been identified by amino acid sequence analysis as the products of the Dictyostelium myosin-IB (DMIB) and myosin-ID (DMID) genes. DMIB, with a specific K+EDTA-ATPase activity 10-fold higher than DMID, was responsible for most of the activity in cell extracts. The third isozyme, present in low amounts, had an apparent molecular mass of 137 kDa on SDS gels and is too large to be the product of any of the known myosin-I genes. DMIB eluted from DE53 cellulose columns as two distinct peaks (II and III). Addition of the phosphatase inhibitor okadaic acid to the extraction buffer increased the fraction of DMIB recovered from growth phase cells in peak III from 35 to 70%. DMIB isolated from peak III, but not from peak II, displayed a significant level of actin-activated MgATPase activity. These results indicate that peak III represents a phosphorylated, actin-activatable form of DMIB.     

Thermal Generation of Residual Stress Fields for Purpose of Distortion Minimization

Thermal treatments of steel components with the goal of hardening often result in distortion by releasing the residual stresses which were brought into the specimen during the preceding processing steps. The goal of the presented work is the minimization of this distortion. By generating definite residual stress fields and investigating the resulting distortion, the distortion mechanism can be observed in detail. A flexible and reproducible way to generate such residual stress fields inside a specimen is by means of local thermal treatment with a laser beam. Computer simulations as well as experiments were carried out using an idealized tooth of a gearwheel (finger sample) as a model system. The deformation of the samples due to the laser heat treatment and the stress fields generated inside the samples were determined with respect to different process parameters.     

Nickel and chromium cycles: Stocks and flows project part IV

Nickel and chromium are essential ingredients in alloys increasingly important for energy-efficient, environmentally friendly modern technology. Quantitative assessment of the flows of these metals through the world economy from resource extraction to final disposal informs resource policy, energy planning, environmental science, and waste management. This article summarizes the worldwide technological cycles of nickel and chromium in 2000. Stainless steel is the major use of these metals, but they serve numerous other special needs, as in superalloys for high-temperature service, as plating materials, and in coinage. Because they are used primarily in alloys, novel recycling issues arise as their use becomes more widespread. “... the great New York and St. Louis double track, nickel plated railroad...” — Norwalk, Ohio, Chronicle 10 March 1881 announces arrival of surveyors for the future Nickel Plate Railway “Later [1911] I formed an alloy of Iron and Chromium, which showed remarkable resistance to rust and tarnish ... [It was] rediscovered by an Englishman named Brearley, in 1914.” —Elwood Haynes to Stephen F. Roberts, 17 January 1925