Robustness of an aerobic metabolically vinyl chloride degrading bacterial enrichment culture

Degradation of the lower chlorinated ethenes is crucial to the application of natural attenuation or in situ bioremediation on chlorinated ethene contaminated sites. Recently, within mixtures of several chloroethenes as they can occur in contaminated groundwater inhibiting effects on aerobic chloroethene degradation have been shown. The current study demonstrated that metabolic vinyl chloride (VC) degradation by an enrichment culture originating from groundwater was not affected by an equimolar concentration (50 μM) of cis-1,2-dichloroethene (cDCE). Only cDCE concentrations at a ratio of 2.4:1 (initial cDCE to VC concentration) caused minor inhibition of VC degradation. Furthermore, the degradation of VC was not affected by the presence of trans-1,2-dichloroethene (tDCE), 1,1-dichloroethene (1,1-DCE), trichloroethene (TCE), and tetrachloroethene (PCE) in equimolar concentrations (50 μM). Only cDCE and tDCE were cometabolically degraded in small amounts. The VC-degrading culture demonstrated a broad pH tolerance from 5 to 9 with an optimum between 6 and 7. Results also showed that the culture could degrade VC concentrations up to 1,800 μM (110 mg/L).     

Taking science education beyond schooling

Abstract

The shape and content of science education has always been articulated from the scientist's perspective. Recently, an increasing number of science educators have recognized the serious limitations of this perspective and have proposed to define scientific literacy from societal or citizenship perspectives. In this article, I argue that these alternatives will remain as limited as their predecessors unless the very structures of schooling are called into question. Furthermore, I argue that alternatives cannot be designed at the drawing board by theoreticians, but have to be grounded in praxis, thereby providing concrete trajectories along which science education can actually change.     

Examining the dimensionality of genuine multipartite entanglement

Entanglement in high-dimensional many-body systems plays an increasingly vital role in the foundations and applications of quantum physics. In the present paper, we introduce a theoretical concept which allows to categorize multipartite states by the number of degrees of freedom being entangled. In this regard, we derive computable and experimentally friendly criteria for arbitrary multipartite qudit systems that enable to examine in how many degrees of freedom a mixed state is genuine multipartite entangled.     

Processing and interactive editing of huge point clouds from 3D scanners

This paper describes a new out-of-core multi-resolution data structure for real-time visualization, interactive editing and externally efficient processing of large point clouds. We describe an editing system that makes use of the novel data structure to provide interactive editing and preprocessing tools for large scanner data sets. Using the new data structure, we provide a complete tool chain for 3D scanner data processing, from data preprocessing and filtering to manual touch-up and real-time visualization. In particular, we describe an out-of-core outlier removal and bilateral geometry filtering algorithm, a toolset for interactive selection, painting, transformation, and filtering of huge out-of-core point-cloud data sets and a real-time rendering algorithm, which all use the same data structure as storage backend. The interactive tools work in real-time for small model modifications. For large scale editing operations, we employ a two-resolution approach where editing is planned in real-time and executed in an externally efficient offline computation afterwards. We evaluate our implementation on example data sets of sizes up to 63 GB, demonstrating that the proposed technique can be used effectively in real-world applications.     

Particle Level Set Advection for the Interactive Visualization of Unsteady 3D Flow

Typically, flow volumes are visualized by defining their boundary as iso‐surface of a level set function. Grid‐based level sets offer a good global representation but suffer from numerical diffusion of surface detail, whereas particle‐based methods preserve details more accurately but introduce the problem of unequal global representation. The particle level set (PLS) method combines the advantages of both approaches by interchanging the information between the grid and the particles. Our work demonstrates that the PLS technique can be adapted to volumetric dye advection via streak volumes, and to the visualization by time surfaces and path volumes. We achieve this with a modified and extended PLS, including a model for dye injection. A new algorithmic interpretation of PLS is introduced to exploit the efficiency of the GPU, leading to interactive visualization. Finally, we demonstrate the high quality and usefulness of PLS flow visualization by providing quantitative results on volume preservation and by discussing typical applications of 3D flow visualization.     

Biofidelic child head FE model to simulate real world trauma

Biomechanics of human head has been widely studied since several decades. At a mechanical level, the use of engineering allowed investigating injury mechanisms developing numerical models of adult head. For children, the problem is more difficult and evaluating child injury mechanisms using data obtained from scaling adult injury criteria does not account for differences in morphology and structure between adults and children. During growth, child head undergoes different modifications in morphology and structure. The present paper compares the anthropometry and numerical simulations of a child head model based on medical CT scans to a child head model developed by scaling an adult head model using the method proposed by Mertz [H.J. Mertz, A procedure for normalizing impact response data, SAE paper 840884, 1984]. These analysis point out significant differences showing that scaling down an adult head to obtain a child head does not appear relevant. Biofidelic and specific child geometry is needed to investigate child injury mechanisms.     

Automatic Construction and Verification of Isotopy Invariants

We extend our previous study of the automatic construction of isomorphic classification theorems for algebraic domains by considering the isotopy equivalence relation. Isotopism is an important generalisation of isomorphism, and is studied by mathematicians in domains such as loop theory. This extension was not straightforward, and we had to solve two major technical problems, namely, generating and verifying isotopy invariants. Concentrating on the domain of loop theory, we have developed three novel techniques for generating isotopic invariants, by using the notion of universal identities and by using constructions based on subblocks. In addition, given the complexity of the theorems that verify that a conjunction of the invariants form an isotopy class, we have developed ways of simplifying the problem of proving these theorems. Our techniques employ an interplay of computer algebra, model generation, theorem proving, and satisfiability-solving methods. To demonstrate the power of the approach, we generate isotopic classification theorems for loops of size 6 and 7, which extend the previously known enumeration results. This work was previously beyond the capabilities of automated reasoning techniques. The author’s work was supported by EPSRC MathFIT grant GR/S31099.     

Production of two-material micro-assemblies by two-component powder injection molding and sinter-joining

In the field of micro-technology the production of metallic and ceramic micro-components by powder injection molding (PIM) has become a more and more established fabrication method. But in order to fulfill the demand for more complex-shaped high-precision micro-components further development work has to be performed. This is especially true if more efficient production routes for multi-component-micro-assemblies consisting of different materials or sub-components are envisaged. To meet these challenges, investigations are performed to realize and to establish two primary shape micro-processes. These are two-component micro-injection molding (2C-MicroPIM) and sinter-joining. The realization of these technologies will lead to a markedly reduction of the efforts for handling, adjustment, and assembling of metallic and ceramic micro-assemblies. Furthermore, an increased integration level and functionality can be yielded. For an effective transfer of scientific results to industrial applications the whole process chain must be considered, from development and construction of the tooling as well as of the components to the quality assurance and determination of the properties of the assemblies after sintering. These primary shape processes shall enable the mutual processing of different materials within the fabrication process, so avoiding separate mounting or assembling steps. Additionally fixed and loose junctions between at least two components shall be realized. The progress in research and development will be demonstrated especially by the implementation of shaft-to-collar connections between micro-gearwheels and corresponding shafts. Regarding two-component micro-injection molding, the tool construction for shaft-to-collar connections will be presented as well as first experimental results on the properties of selected ceramic powders and feedstocks for the special requirements of the 2C-MicroPIM process. With the assembly step being performed outside the injection molding tool before sinter-joining different parts and geometries can be combined quite easily. The presented article gives an overview on the concept and on preliminary testing results for the fabrication of a shaft-to-collar-connection. Additionally, a solution for an automated assembly of a shaft and a toothed wheel outside the injection molding tool is presented.     

Improving SCTP retransmission delays for time-dependent thin streams

A large number of network services rely on IP and reliable transport protocols. For applications that provide abundant data for transmission, loss is usually handled satisfactorily, even if the application is latency-sensitive (Wang et al. 2004). For data streams where small packets are sent intermittently, however, applications can occasionally experience extreme latencies (Griwodz and Halvorsen 2006). As it is not uncommon that such thin-stream applications are time-dependent, any unnecessarily induced delay can have severe consequences for the service provided. Massively Multiplayer Online Games (MMOGs) are a defining example of thin streams. Many MMOGs (like World of Warcraft and Age of Conan) use TCP for the benefits of reliability, in-order delivery and NAT/firewall traversal. It has been shown that TCP has several shortcomings with respect to the latency requirements of thin streams because of the way it handles retransmissions (Griwodz and Halvorsen 2006). As such, an alternative to TCP may be SCTP (Stewart et al. 2000), which was originally developed to meet the requirements of signaling transport. In this paper, we evaluate the Linux-kernel SCTP implementation in the context of thin streams. To address the identified latency challenges, we propose sender-side only enhancements that reduce the application-layer latency in a manner that is compatible with unmodified receivers. These enhancements can be switched on by applications and are used only when the system identifies the stream as thin. To evaluate the latency performance, we have performed several tests over various real networks and over an emulated network, varying parameters like RTT, packet loss and amount of competing cross traffic. When comparing our modifications with SCTP on Linux and FreeBSD and TCP New Reno, our results show great latency improvements and indicate the need for a separate handling of thin and thick streams.