Transitory urbanites: New actors of residential change in Polish and Czech inner cities

For decades inner cities in East Central Europe have been characterised by physical dilapidation, ageing in place and selective outmigration. Post-socialist transition added to these developments by further outmigration due to suburbanisation but also provided new opportunities of residential change. In Polish and Czech second-order cities there is selective in-migration by younger households which remains so far almost hidden to the official statistics. Among them a new type of inner-city dwellers can be identified, that is transitory urbanites. They take the inner city as a perfect residential place in their current phase of life and value its characteristics both in emotional and pragmatic terms. But they do not expect to stay there for good and might leave the inner city in the future for another urban area or suburbia. However, while being present now they bring about residential change in the inner city and pave the way for future in-migrants of similar social status and age. From our material and observations, we can formulate the hypothesis that their importance has increased decisively during the last decade. Our paper draws on empirical research from in ?ód? and Brno from 2006 to 2009.     

Hydrogen and Zr-based metallic glasses: Gas/solid absorption process and structure evolution

The absorption of hydrogen by means of gas-solid reaction and its consequence on the structure have been studied for fully amorphous alloys as well as quasicrystals/glassy composite alloys based on the composition Zr₅₉Ti₃Cu₂₀Al₁₀Ni₈. The process of hydrogen absorption has been performed and monitored under 20 bar of H₂ using high pressure-differential scanning calorimetry (HP-DSC). The structure evolution of the samples has been followed by X-ray diffractometry (XRD). Results show that the nature of the surface oxide layer strongly affects the process of hydrogen absorption, especially its starting temperature. The structure evolves nevertheless along the same basic sequence, regardless of the sample: (i) the alloys keep a global amorphous structure up to roughly H/M = 0.8 and T = 350 °C; (ii) then ZrH₂ and at higher temperature Cu₂AlZr are formed. The stability of the glass is weakened and the formation of quasicrystals is inhibited under 20 bar of H₂. An heterogeneous distribution of hydrogen atoms inside the amorphous matrix has been inferred from the results.     

Contribution on the influence of transformation structures under consideration of austenite deformation

Hot forming represents a substantial part of the manufacturing process of many steel products. The crucial microstructural transformation process, which influences the characteristics of a steel considerably, is undisputed the γ/α‐transformation. A successful aid to the interpretation of possible microstructural transformation processes during cooling from the austenitizing or deformation temperature is the determination and handling of CCT diagrams. In this article two steels are examined which differ only in their titanium content. Dilatometric investigations were performed varying the three parameters' transformation temperature, strain and cooling strategy. The insights gathered are illustrated and discussed with the aid of CCT diagrams. An additional transformation below A_c1 with a brief curing reheat up to A_c3 + 20 K leads to a substantially finer structure. The exploitation of this effect may be of great industrial importance.     

Non-Reproducibility of Oral Rotenone as a Model for Parkinson’s Disease in Mice

Oral rotenone has been proposed as a model for Parkinson’s disease (PD) in mice. To establish the model in our lab and study complex behavior we followed a published treatment regimen. C57BL/6 mice received 30 mg/kg body weight of rotenone once daily via oral administration for 4 and 8 weeks. Motor functions were assessed by RotaRod running. Immunofluorescence studies were used to analyze the morphology of dopaminergic neurons, the expression of alpha-Synuclein (α-Syn), and inflammatory gliosis or infiltration in the substantia nigra. Rotenone-treated mice did not gain body weight during treatment compared with about 4 g in vehicle-treated mice, which was however the only robust manifestation of drug treatment and suggested local gut damage. Rotenone-treated mice had no deficits in motor behavior, no loss or sign of degeneration of dopaminergic neurons, no α-Syn accumulation, and only mild microgliosis, the latter likely an indirect remote effect of rotenone-evoked gut dysbiosis. Searching for explanations for the model failure, we analyzed rotenone plasma concentrations via LC-MS/MS 2 h after administration of the last dose to assess bioavailability. Rotenone was not detectable in plasma at a lower limit of quantification of 2 ng/mL (5 nM), showing that oral rotenone had insufficient bioavailability to achieve sustained systemic drug levels in mice. Hence, oral rotenone caused local gastrointestinal toxicity evident as lack of weight gain but failed to evoke behavioral or biological correlates of PD within 8 weeks.     

Single- and Multiscale Laser Patterning of 3D Printed Biomedical Titanium Alloy: Toward an Enhanced Adhesion and Early Differentiation of Human Bone Marrow Stromal Cells

This study explores the enhancement of biocompatible titanium-based implants through surface functionalization for improved bone healing. Specifically, a near-beta type Ti-13Nb-13Zr alloy is 3D printed using laser powder bed fusion and subsequently textured using nanosecond (ns) and picosecond (ps) direct laser interference patterning (DLIP) to create single-scale and multi-scale surface textures. On these textures, the cell behavior, morphology, metabolic activity and osteogenic differentiation potential of human bone marrow stromal cells are assessed using fluorescence microscopy and MTS assays. Moreover, tissue non-specific alkaline phosphatase activity served as an early osteoblast production marker. Compared to untextured specimens, both types of textures exhibited higher metabolic activity and cell proliferation. Single-scale ns-DLIP textures encouraged cell extensions anchored in groove regions, while ps-DLIP textures with hierarchical structures promoted cell extensions attaching to nanostructures on sidewalls. The groove width and nanotopographies in groove areas facilitated cell spreading. Surface topography, roughness, and surface chemistry (surface energy, wettability) influenced cell adhesion, proliferation, and differentiation. A comprehensive evaluation of DLIP-generated surface textures, including their topography and chemical states, complements the factors affecting in vitro cell behavior. Overall, this research demonstrates the potential of surface-functionalized 3Dprinted titanium for a novel generation of biocompatible implants.     

Monitoring freeze/thaw cycles using ENVISAT ASAR Global Mode

Due to the high temporal sampling rate of ASAR Global Monitoring (GM) mode, it has a high application potential for analyzing the land surface freeze/thaw process in high latitudes. This study aims to develop effective methods of extracting freeze/thaw transition dates of permafrost areas from ASAR GM data sets. In order to use ASAR GM time-series for analyzing freeze/thaw states, a least square fitting of piecewise step function is introduced. The thawing date can be determined by minimizing the sum of squared residuals between measured backscattering time-series and a pre-defined step function. An experimental result for a Siberian permafrost region illustrates that it can be a promising approach in monitoring permafrost ecosystems.     

Synthesis and physical characterization of magnetite nanoparticles for biomedical applications

Iron oxide nanoparticles for biomedical applications in the size range of 15–130 nm were prepared by either oxidative hydrolysis of ferrous sulfate with KOH or precipitation from ferrous/ferric chloride solutions. The magnetite particle size is controlled by variation of pH and temperature. The synthesized magnetite nanoparticles are partially oxidized as signaled by ferrous concentrations of below 24 wt% Fe²⁺ and lattice parameters of a₀ ≤ 8.39 Å which are smaller compared to 8.39 Å for stoichiometric magnetite. The extend of oxidation increases with decreasing particle size. Heating at 150–350 °C topotactically transforms the magnetite nanoparticles into stoichiometric tetragonal maghemite (ferrous ion concentration c_Fe²⁺=0 and a₀ = 8.34 Å) without significant particle growth. The magnetite–maghemite transformation is studied with thermal analysis, XRD and IR spectroscopy. The saturation magnetizations of the magnetite and maghemite particles decrease with decreasing particle size. The variation of M_s with particle size is interpreted using a magnetic core–shell particle model. Magnetite particles with d ≤ 16 nm show superparamagnetic behavior at room temperature whereas particles with diameter >16 nm display hysteresis behavior. These particles are candidates for biomedical applications, e.g. controlled drug release or hyperthermia.     

Electrocrystallisation of metallic films under the influence of an external homogeneous magnetic field—Early stages of the layer growth

The effects of a uniform magnetic field on the early stages of Ag, Fe and CoFe alloys electrocrystallisation have been investigated. It was found for Fe and CoFe alloys, irrespective of the applied parameters, that early stages of the layer growth can be characterised by a nucleation and 3D diffusion controlled growth. The influence of the deposition parameters on the nucleation behaviour was studied on the basis of the Sharifker-Mostany (SM) model. A modification to the existing model has been proposed in order to model alloy systems. It is reported that a magnetic field superposed parallel to the electrode surface has a significant influence on the early stages of Fe and CoFe alloys growth. The growth of the nuclei is enhanced by the Lorentz-force-driven convection, while the nucleation processes remain unaffected. The hydrodynamic origin of these phenomena is confirmed by independent rotating disk electrode (RDE) investigations. Moreover, the proposed mechanism of a magnetic field influence on the 3D diffusion controlled growth is supported by a microscopic investigation of Ag deposits. It was found that Ag deposits obtained without a magnetic field superposition are characterised by a relatively large number of small 3D growth centres, whereas the deposits obtained in a field show fewer 3D centres but their size is greatly increased.