Enhanced expression of cyclin D1 in senescent human fibroblasts

When human fibroblast, TIG-1, was growth-stimulated with fetal bovine serum, the induction level of cell cycle-dependent genes was generally much lower in senescent cells than in young counterparts. Exceptionally, the expression level of cyclin D1 in senescent cells was constitutively higher than in young cells and further increased after serum stimulation, which was confirmed by Northern and Western blots and immunoprecipitation. This was also true in other human diploid fibroblast lines, TIG-3 and MRC-5. However, cyclin D1-dependent kinase activity was not detected in senescent cells. When sense- or antisense-cyclin D1 cDNA driven by beta-actin promoter was transfected into young TIG-1 cells, the number of appeared colonies from sense-strand transfected cultures was lower than that from antisense-strand-transfected ones. However, clones expressing cyclin D1 at low or undetectable level which were isolated after transfection with antisense-cyclin D1 proliferated up to the same division limit as untransfected and sense-strand transfected cells. Four clones of SV40-transformed TIG-1 expressed cyclin D1 at moderate levels during their extended proliferative lifespan. It appears that, if the extremely overexpressed cyclin D1 could cause an inhibition of cell proliferation at senescent stage, cellular senescence occurs regardless of overexpression of cyclin D1.     

Visualization of coronary arteries with helical scanning CT: diastolic reconstruction

The purpose of this study was the visualization of coronary arteries by the diastolic reconstruction method with helical scanning (HES) CT. We chose diastolic phase images from the image data acquired with the HES technique using a continuous nutate-rotate fast CT scanner, because few motion artifacts are induced by cardiac pulsation during the slow filling diastolic phase. We assessed coronary diseases using this method based on HES-CT, and evaluated its clinical effectiveness. We also studied the clinical value of multiplanar reconstruction and three-dimensional surface reconstruction. The subjects consisted of 31 patients with coronary disease, aged 41-77 years. When a diastolic reconstruction HES-CT was employed, the average rate of visualization of the coronary arteries was 72%. An examination can be completed during a 30-second single breath-hold, permitting the acquisition of coronary artery images with excellent continuity. Calcified coronary arteries can be identified.     

The behavior of charged conducting particles in electric fields

The presence of metal particles within a gas-insulated switchgear (GIS) might initiate a sparkover and lead to fatal failure of an electric power transmission system. In order to investigate the behavior of a particle in a GIS, the model electrodes and the measuring system are constructed. The motion of a particle was investigated by using a CCD camera and a high-speed video camera. Different shapes of elongated particles were made from 0.1-mm and 0.25-mm diameter wires. The measured values of lift-off field strength of a particle lying on the electrode agreed quite well with the theoretical predictions. The initial raising up of a particle usually started at the sharper edge, as expected. There were two types of different modes of particle motions: one is the bouncing motion between two electrodes or one electrode, and the other is the standing motion on the electrode. An interesting thing is that the standing motion is on the upper electrode, when a negative voltage is applied to the upper electrode. This phenomenon could be explained from the difference of positive and negative corona currents starting from both edges of a standing particle     

Compositional Dependability Evaluation for STATEMATE

Software and system dependability is getting ever more important in embedded system design. Current industrial practice of model-based analysis is supported by state-transition diagrammatic notations such as Statecharts. State-of-the-art modelling tools like STATEMATE support safety and failure-effect analysis at design time, but restricted to qualitative properties. This paper reports on a (plug-in) extension of STATEMATE enabling the evaluation of quantitative dependability properties at design time. The extension is compositional in the way the model is augmented with probabilistic timing information. This fact is exploited in the construction of the underlying mathematical model, a uniform continuous-time Markov decision process, on which we are able to check requirements of the form: The probability to hit a safety-critical system configuration within a mission time of 3 hours is at most 0.01. We give a detailed explanation of the construction and evaluation steps making this possible, and report on a nontrivial case study of a high-speed train signalling system where the tool has been applied successfully.     

Potential of Monoraphidium sp. GK12 for energy‐saving astaxanthin production

BACKGROUND: Astaxanthin is a chemical used in aquaculture, pharmaceuticals, foods, and cosmetics. Although Haematococcus pluvialis has been studied intensively as an astaxanthin producer, its cultivation requires stringent contamination control and nutrition levels, thus rendering astaxanthin production energy‐consuming. In the present study, the medium composition for cultivation of Monoraphidium sp. GK12, a newly isolated astaxanthin‐producing microalga, was improved and photoautotrophic cultivation was attempted to examine its potential for application to energy‐saving astaxanthin production. RESULTS: A cost‐saving medium composed only of mineral salts was found to be suitable for photoautotrophic GK12 cultivation. A specific growth rate of 0.57 day^?1 was obtained, which is higher than that of H. pluvialis. A high cell density of around 2.0 × 10⁷ cells mL^?1 was accomplished in outdoor batch cultivation, while outgrowth of contaminants was not observed. GK12 was also cultivated in a continuous photobioreactor, and the introduction of 1% CO₂ stimulated GK12 growth and astaxanthin productivity. CONCLUSION: Control of temperature, illumination, and culture pH is unnecessary for GK12 outdoor cultivation and no outgrowth of contaminants was observed, suggesting an advantage over H. pluvialis outdoor cultivation. Besides improving Haematococcus cultivation methods, research on GK12 as an alternative astaxanthin‐producer may contribute to the establishment of energy‐saving astaxanthin production. Copyright © 2008 Society of Chemical Industry     

Effects of temperature and composition on the laminar burning velocity of CH4 + H2 + O2 + N2 flames

This work summarises available measurements of laminar burning velocities in CH₄ + H₂ + O₂ + N₂ flames at atmospheric pressure performed using a heat flux method. Hydrogen content in the fuel was varied from 0% to 40%, amount of oxygen in the oxidiser was varied from 20.9% down to 16%, and initial temperature of the mixtures was varied from 298 to 418 K. These mixtures could be formed when enrichment by hydrogen is combined with flue gas recirculation. An empirical correlation for the laminar burning velocity covering a complete range of these measurements is derived and compared with experiments and other correlations from the literature.     

Relation of electrocardiographic left ventricular hypertrophy with and without T-wave changes to systemic blood pressure, body mass, and serum lipids and blood glucose levels in Japanese men

Left ventricular (LV) hypertrophy, especially combined with an abnormal ST-T, is considered 1 of many coronary risk factors. Seven hundred forty-nine Japanese men were selected according to their electrocardiographic findings, i.e., normal electrocardiogram, LV hypertrophy without an abnormal ST-T segment, LV hypertrophy with a flat T wave, and LV hypertrophy with a negative T wave. Coronary risk factors were compared among these 4 age-matched groups. Groups with LV hypertrophy with negative or flat T waves had larger body mass index (24.9 vs 22.9 kg/m2), higher mean systemic blood pressure (111 vs 95 mm Hg), larger LV mass (265 vs 157 g), higher blood glucose (110 vs 100 mg/dl), higher serum triglyceride (148 vs 122 mg/dl), higher total cholesterol (206 vs 198 mg/dl), and lower high-density lipoprotein cholesterol (47 vs 54 mg/dl) than the normal group or the group with LV hypertrophy without T-wave change. Among these risk factors, blood pressure and glucose remained higher even after the adjustment by body mass index or by body mass index and blood pressure. Electrocardiographic LV hypertrophy with a changed T wave signified higher risk of coronary artery disease in Japanese men.     

Multishot echoplanar MR imaging of the female pelvis: comparison with fast spin-echo MR imaging in an initial clinical trial

OBJECTIVE: The purpose of this study was to apply multishot echoplanar MR imaging (EPI) to the female pelvis and compare image quality with that of fast spin-echo MR imaging. SUBJECTS AND METHODS: Twenty-one patients with suspected pelvic disease and five healthy female volunteers were prospectively examined. MR imaging was obtained using an EPI-capable 1.0-T imager and a pelvic phased-array coil. Axial EPI and fast Spin-echo sequences were obtained at identical image locations in each patient and volunteer. Spin-echo EPI images were obtained using a multishot number of 16. Acquisition time for each EPI sequence was 2 min 10 sec. Fat-suppressed T2-weighted fast spin-echo images were obtained in 2 min 12 sec. Paired EPI and fast spin-echo MR images were independently evaluated by three reviewers. RESULTS: Delineation of the perivaginal and parametrial venous plexus on EPI was rated superior to the fast spin-echo images in 62 (77%) of 81 cases. On EPI, uterine zone anatomy and ovary visualization were judged to be inferior in 44 (56%) of 78 cases and in 18 (33%) of 54 cases, respectively. For delineation of uterine abnormalities, including leiomyoma and adenomyosis, both sequences performed almost equally well. However, ovarian cystic lesions were revealed more precisely by the fast spin-echo sequence. An overall fat-suppression effect was seen on EPI images in 62 (77%) of 81 cases. CONCLUSION: Multishot EPI cannot replace fast spin-echo sequences for imaging the female pelvis; however, because EPI has a potent fat-suppression effect and heavily T2-weighted contrast, EPI sequences can be a valuable adjunct to routine examination.     

Modelling the effects of a snowdrift-preventing forest and a snow fence by means of activated clay particles

This paper presents experimental results obtained in a wind tunnel concerning the drift geometry of snow formed around models of a snowdrift-preventing forest and a snow fence, using activated clay particles as a substitute for natural snow. They gave a well defined simulation of the drift geometry of snow, whereas other materials such as MgO₂, Al₂O₃, sawdust and mica flakes were unsuccessful in providing good results.Moiré photography was used for measuring the two dimensional distribution of depth of clayparticle deposits formed around structures without causing any disturbances to the topography of the snow surface.