Time course of fetal tissue invasion by Listeria monocytogenes following an oral inoculation in pregnant guinea pigs

Pregnant women are 20 times more likely to develop listeriosis than are members of the general population, and infection can result in abortion, stillbirth, or neonatal illness. The objective of this study was to orally challenge pregnant guinea pigs with Listeria monocytogenes to assess maternal and fetal tissue invasion at postinoculation days 2, 6, 9, and 21. The time course of invasion was followed by fluorescence microscopy and a traditional culture method. Guinea pigs were treated on gestation day 35 with L. monocytogenes doses ranging from 10(4) to 10(8) CFU. L. monocytogenes was isolated and viewed in maternal and fetal tissues as early as 2 days postinoculation. L. monocytogenes was isolated from placentas, fetal livers and brains, and maternal spleens at similar rates, suggesting that invasion of the spleen could be indicative of fetal invasion. When comparing fecal shedding, all animals treated with 10(4) CFU were shedding L. monocytogenes by postinoculation day 7, and all animals treated with the higher doses (10(6) or 10(8) CFU) were shedding L. monocytogenes by postinoculation day 5. These data suggest that L. monocytogenes crosses the fetoplacental barrier and invades the fetus by day 2 after maternal ingestion. When comparing the sensitivities of microscopy and culture, neither method consistently detected L. monocytogenes at a higher rate. However, detection in individual tissues differed. Microscopy was significantly more sensitive with fetal liver (P<0.001) and brain (P<0.001) at the highest dose of 10(8) CFU, but at the lowest dose of 10(4) CFU culture was significantly more sensitive with maternal spleen (P=0.04).     

Coordinates Versus Sequential Facsimile

The purpose of this paper is to quantitatively evaluate the improvement that can be obtained, in the coding of graphics, by using more sophisticated encoding schemes than run-length. These schemes are now well within the reach of today's technology. In the broad class of encoders by coordinates, three isoentropic encoding schemes are considered: 1) encoding of isolated points, 2) encoding of unidimensional patterns, 3) encoding of two-dimensional patterns. Although the three systems are all conceptually straightforward, they require an increasing complexity of encoding-decoding operations. This complexity is balanced, however, by a larger compression factor for the more complex schemes. The results obtained with these encoding schemes are finally compared to those of a run-length coding scheme for the case of a first-order Markov model of the source.     

Analysis of Left Ventricular Mechanics During Filling, Isovolumic Contraction, and Ejection

The left ventricle (LV) was modeled by two confocal ellipsoids truncated in a plane corresponding to the base of the LV. The ellipsoids were approximated by a series of cylindrical shells. During passive filling, the pressure within the ventricular chamber was determined from chamber volume and a stress-strain relationship for myocardium in the relaxed state. The rapid filling phase of diastole was not analyzed. During isovolumic contraction, the cylindrical shells assumed the properties of myocardium in active contraction. Contraction was sequential, beginning at the ventricular apex, and progressing toward the ventricular base. Geometric changes occurred in the LV model as a function of wall stress, material properties, and timing of myocardial activation. During ejection, viscous and inertial forces were determined as were model output pressure and flow waveforms.     

Calculating the effective pattern rate for high-speed board testapplications

A complex interplay of tester specifications can force in-circuit and functional board test systems to operate at less than their specified maximum pattern rates in real-world test applications. The author explores the factors that combine to limit test speed. He develops models for calculating the effective pattern rate based on tester performance data and the characteristics of the VLSI board under test     

Watermarking on CNN‐UM for image and video authentication

In this paper a new approach to fragile watermarking technique is introduced. This problem is particularly interesting in the field of modern multimedia applications, when image and video authentication are required. The approach exploits the cellular automata suitability to work as pseudorandom pattern generators and extends the related algorithms under the framework of the cellular non‐linear networks (CNNs). The result is a novel way to perform watermarking generation in real time, using the presently available CNN‐universal chip prototypes. In this paper, both the CNN algorithms for fragile watermarking as well as on‐chip experimental results are reported, confirming the suitability of CNNs to successfully act as real‐time watermarking generators. The availability of CNN‐based visual microprocessors allows to have powerful algorithms to watermark in real time images or videos for efficient smart camera applications. Copyright © 2004 John Wiley & Sons, Ltd.     

Home- versus clinic-based treatment of vascular headache.

Contrasted the efficacy of a traditional clinic-based relaxation training and thermal biofeedback treatment to a comparable home-based program with minimal therapist contact in the treatment of 40 vascular-headache sufferers (mean age 37 yrs). The home-based condition was at least as efficacious as the clinic-based treatment in terms of reduction of headache intensity and frequency and medication usage, and was clearly more cost-effective. Findings suggest that a minimal-therapist-contact condition may be a viable, low-cost alternative to more expensive clinic-based programs in the treatment of vascular headache. (14 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Peptide Materials Obtained by Aggregation of Polyphenylalanine Conjugates as Gadolinium‐Based Magnetic Resonance Imaging Contrast Agents

Peptide materials based on the aggregation of polyphenylalanine conjugates containing gadolinium complexes and acting as potential contrast agents (CAs) in magnetic resonance imaging (MRI) are described. Monomers contain two (F2) or four (F4) phenylalanine residues for self‐assembly, a chelating agent, 1,4,7,10‐tetraazacyclododecane‐N,N,N,N‐tetraacetic acid (DOTA) or diethylenetriaminepentaacetic acid (DTPA), for achieving gadolinium coordination, and ethoxylic linkers at two (L₂) or six (L₆) poly(ethylene glycol) (PEG) units between the chelating group and the peptide region. Both DOTA and DTPA tetraphenylalanine derivatives, and their gadolinium complexes DOTA(Gd)‐L₆‐F4 and DTPA(Gd)‐L₆‐F4, are able to self‐aggregate at very low concentration. Structural characterization, obtained by circular dichroism and infrared measurements, confirms the amyloid type fibril formation in which an antiparallel peptide alignment is preferred. Amyloid type fibril formation is also observed, in solid state, by transmission electron microscopy images and X‐ray diffraction patterns. The relaxivity values of DOTA(Gd)‐L₆‐F4 and DTPA(Gd)‐L₆‐F4 and their ability to enhance the MRI cellular response on the J774A.1 mouse macrophages cell line indicate that these peptide materials are promising candidate as a new class of supramolecular gadolinium based MRI contrast agents.     

A study of the behaviour of Pt supported on CeO2–ZrO2/Al2O3–BaO as NOx storage–reduction catalyst for the treatment of lean burn engine emissions

The behaviour of a Pt(1 wt.%) supported on CeO₂–ZrO₂(20 wt.%)/Al₂O₃(64 wt.%)–BaO(16 wt.%) as a novel NO_x storage–reduction catalyst is studied by reactivity tests and DRIFT experiments and compared with that of Pt(1%)–BaO(15 wt.%) on alumina. The former catalyst, designed as a hydrothermally stable sample, is composed of an alumina modified with Ba ions and an overlayer of ceria-zirconia. The results pointed out that during the calcination barium ions migrates over the surface of the catalyst which thus show a good NO_x storage–reduction behaviour comparable with that of Pt–BaO on alumina, although Ba ions result much better dispersed.