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301.
Swachhatoa Ghosh Dr. Prem Singh Shounak Roy Keshav Bhardwaj Dr. Amit Jaiswal 《Chembiochem : a European journal of chemical biology》2022,23(8):e202100691
Nanozymes are artificial enzyme systems which are easy to produce, highly stable and cost-effective in comparison to natural enzymes. Herein, we evaluated the peroxidase-like activity of gold nanorattles (Au NRTs) having a solid gold octahedron core and thin, porous cubic gold shell. We also prepared solid gold nanocubes and nanospheres of similar sizes and surface charge as that of Au NRTs and compared their activity with standard horse radish peroxidase (HRP). All the prepared nanostructures followed Michaelis-Menten kinetics as observed from their substrate concentration vs. initial reaction velocity plot using 3,3’,5,5’-tetramethylbenzidine (TMB) as a substrate. The kinetic parameters and the catalytic efficiency for the peroxidase-like activity of the nanostructures and HRP were calculated, and it was observed that Au NRTs possess the best nanozymatic activity with lowest KM and highest catalytic efficiency (kcat/KM). The better activity of Au NRTs compared with other nanostructures and HRP could be attributed to the hollow porous structure with a solid core where different surfaces are available for reaction. Au NRTs, being the best amongst the tested nanozymes were further used for the sensing of hydrogen peroxide (H2O2) and were found to sense H2O2 down to 0.5 μM. Further, two naturally occurring antioxidants, tannic acid and ascorbic acid showed inhibitory effect on the peroxidase-like activity of Au NRTs in a concentration dependent manner which can be further used for screening of antioxidants or for determining the antioxidant potential. 相似文献
302.
Artificial Life and Robotics - In recent years, many countries including Japan are facing the problems of increasing old-age population and shortage of labor. This has increased the demands of... 相似文献
303.
Nigam Kaushal Kondekar PN Chandan Bandi Venkata Kumar Satyendra Tikkiwal Vinay Anand Dharmender Singh Km. Sucheta Bhardwaj Eshaan Choubey Shubham Chaturvedi Savitesh 《SILICON》2022,14(4):1549-1558
Silicon - To overcome the fabrication complexity and achieve a better switching ratio is a major grave concern for applications in semiconductor devices. In this regards, a novel stack gate-oxide... 相似文献