Quantitative analysis of sperm rheotaxis using a microfluidic device

Quite puzzling issue in biology is how sperm cells are selected naturally where human sperm has to maintain a correct swimming behavior during the various stages of reproduction process. In nature, sperm has to compete a long journey from cervix to oocyte to stand a chance for fertilization. Although various guidance mechanisms such as chemical and thermal gradients are proposed previously, these mechanisms may only be relevant as sperm reaches very close to the oocyte. Rheotaxis, a phenomenon where sperm cells swim against the flow direction, is possibly the long-range sperm guidance mechanism for successful fertilization. A little is known quantitatively about how flow shear effects may help guide human sperm cells over long distances. Here, we have developed microfluidic devices to quantitatively investigate sperm rheotaxis at various physiological flow conditions. We observed that at certain flow rates sperm actively orient and swim against the flow. Sperm that exhibit positive rheotaxis show better motility and velocity than the control (no-flow condition), however, rheotaxis does not select sperm based on hyaluronic acid (HA) binding potential and morphology. Morphology and HA binding potential may not be a significant factor in sperm transport in natural sperm selection.     

Morphology enhancement of TiO2/PVP composite nanofibers based on solution viscosity and processing parameters of electrospinning method

In this work, different sol solutions with various titanium tetraisopropoxide (TIP)/glacial acetic acid ratios in 2‐propanol with 5 wt % poly(vinyl pyrrolidone) (PVP) (M_w = 360,000 g/mol) were prepared and electrospun. Composition of the prepared sols and as‐spun TiO₂/PVP nanofibers were determined by Fourier transform infrared and Raman spectroscopy methods. Morphology of the electrospun TiO₂/PVP nanofibers was studied by scanning electron microscopy and transmission electron microscopy (TEM) techniques. Rheometry measurements of the sol solutions showed decrease of viscosity upon the addition of TIP to the polymer solutions with constant polymer and acid concentrations. The sol solution having the lowest viscosity (at shear rate 10 s^?1) but the highest TIP/glacial acetic acid ratio showed beaded nanofibers morphology when electrospun under 10 and 12 kV applied voltage while injection rate, needle tip to collector distance, and needle gauge were kept constant. However, smooth electrospun TiO₂/PVP composite nanofibers with the average nanofibers diameters (148 ± 79 nm) were achieved under the same condition when applied voltage increased to 15 kV. TEM micrographs of the electrospun TiO₂/PVP nanofiber showed that the TiO₂ particles with continuous structure are formed at the middle of the nanofiber and distributed along its axis. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 46337.     

Effect of polyethylenimine addition and washing on stability and electrophoretic deposition of Co3O4 nanoparticles

In this work, the parameters of cobalt oxide suspension such as conductivity, zeta potential, particle size, stability, and finally the electrophoretic behavior of particles in the absence and presence of polyethylenimine (PEI) in acetone medium were investigated. Also, the effects of washing on the stability and electrophoretic deposition of Co₃O₄ were studied. Characterization of the obtained layer by optical microscopy revealed that there was no deposition in the suspension without PEI, while a uniform layer was formed in the presence of PEI additive. Scanning electron microscopy (SEM) results confirmed the uniformity of layer obtained in acetone using PEI additive. Moreover, SEM results demonstrated that more porous microstructures were obtained at longer deposition durations. The difference in the porosity of the layers, as indicated by the SEM micrographs, is attributed to increase in the deposition time.     

Effect of various surface preparation techniques on the delamination properties of vacuum infused Carbon fiber reinforced aluminum laminates (CARALL): Experimentation and numerical simulation

Carbon fiber reinforced aluminum laminates (CARALL) are one of the aluminum based Fiber metal laminates (FMLs) which, due to their high strength to weight ratio and good impact resistance are greatly replacing aluminum alloys in aircraft structures. In this research work, interlaminate shear strength of Vacuum assisted resin transfer molding (VARTM) manufactured CARALL has been investigated. Numerical simulation model incorporated with real time material data has been developed to predict the delamination behavior of CARALL laminates. Standard CARALL specimens with different surface morphologies were prepared by electric discharge machining, mechanical, chemical and electrochemical surface treatments. T-peel tests were carried out according to standard ASTM D1876-08 to find out inter laminate shear strength. FMLs made out of mechanically, chemically and electrochemically cleaned metal sheets depicted high interlaminate shear strength. SEM micrographs of failed surfaces verify the high adhesive strength of epoxy. Developed numerical simulation model accurately predicts the delamination behavior of CARALL as observed during experimentation.     

Effect of polishing parameters on abrasive free chemical mechanical planarization of semi-polar(1122) aluminum nitride surface

An abrasive free chemical mechanical planarization(AFCMP) of semi-polar(1122) AlN surface has been demonstrated. The effect of slurry pH, polishing pressure, and platen velocity on the material removal rate(MRR) and surface quality(RMS roughness) have been studied. The effect of polishing pressure on the AFCMP of the(1122) AlN surface has been compared with that of the(1122) AlGaN surface. The maximum MRR has been found to be ~562 nm/h for the semi-polar(1122) AlN surface, under the experimental conditions of 38 kPa pressure, 90 rpm platen velocity, 30 rpm carrier velocity, slurry pH 3 and 0.4 M oxidizer concentration. The best root mean square(RMS) surface roughness of ~1.2 nm and ~0.7 nm, over a large scanning area of 0.70×0.96 mm², has been achieved on AFCMP processed semi-polar(1122) AlN and(AlGaN) surfaces using optimized slurry chemistry and processing parameters.     

The effect of fabric structure on the bursting characteristics of warp-knitted surgical mesh

Abstract

Surgical mesh is a porous fabric that is mainly produced from polymeric monofilaments using the warp-knitting method. Surgical mesh is used for healing hernia. Among the mechanical properties of the mesh, tensile and bursting strength have special significance. In this research, warp-knitted surgical meshes with five structures have been produced using polypropylene monofilament, and the effect of the fabric structure on the bursting characteristics of them has been investigated. All the meshes exhibited supraphysiologic bursting strength. quasi-Sandfly mesh demonstrated the highest, while quasi-Marqussite mesh exhibited the least bursting characteristics. The increase in the bursting characteristics of the meshes is attributed to the increase in the areal density and decrease in the porosity of them. Moreover, conformity between the bursting and tensile properties of them in bias direction has been observed. Overall, quasi-Marqussite mesh was proposed as the most desirable mesh in terms of porosity, weight, and bursting characteristics.     

Optimal design of a modular axial-flux permanent-magnet synchronous generator for gearless wind turbine applications

Air-cored axial-flux permanent-magnet synchronous generators (AFPMSGs) are potential candidates for gearless direct-coupled wind turbines (DCWTs) owing to providing high efficiency and power density. The design of a DCWT generator is so complicated since the generator cost, dimension, and weight affected by gear elimination. Therefore, it is essential to find an optimal AFPMSG design at rated conditions. In this paper, an accurate procedure for the optimal design of an air-cored AFPMSG applicable for DCWTs is proposed. The genetic algorithm (GA) is used for multi-objective design optimization to reach the optimal configuration as well as system dimension in order to decrease the weight, increase the power density and enhance the effectiveness of the generator. To validate the efficiency of the suggested optimization proceducer, a 30 kW AFPMSG has been considered as a case study. The results of optimization have been investigated by finite element analysis (FEA). A prototype generator is also fabricated, and the test results are offered and compared with the numerical study. The outcomes show that there exists an acceptable agreement between FEA and experimental outcomes with the error percentage about of 1.35%.     

CSH RNA Interference Reduces Global Nutrient Uptake and Umbilical Blood Flow Resulting in Intrauterine Growth Restriction

Deficiency of the placental hormone chorionic somatomammotropin (CSH) can lead to the development of intrauterine growth restriction (IUGR). To gain insight into the physiological consequences of CSH RNA interference (RNAi), the trophectoderm of hatched blastocysts (nine days of gestational age; dGA) was infected with a lentivirus expressing either a scrambled control or CSH-specific shRNA, prior to transfer into synchronized recipient sheep. At 90 dGA, umbilical hemodynamics and fetal measurements were assessed by Doppler ultrasonography. At 120 dGA, pregnancies were fitted with vascular catheters to undergo steady-state metabolic studies with the ³H₂O transplacental diffusion technique at 130 dGA. Nutrient uptake rates were determined and tissues were subsequently harvested at necropsy. CSH RNAi reduced (p ≤ 0.05) both fetal and uterine weights as well as umbilical blood flow (mL/min). This ultimately resulted in reduced (p ≤ 0.01) umbilical IGF1 concentrations, as well as reduced umbilical nutrient uptakes (p ≤ 0.05) in CSH RNAi pregnancies. CSH RNAi also reduced (p ≤ 0.05) uterine nutrient uptakes as well as uteroplacental glucose utilization. These data suggest that CSH is necessary to facilitate adequate blood flow for the uptake of oxygen, oxidative substrates, and hormones essential to support fetal and uterine growth.     

A mixed analytical-numerical investigation of snap-through of low arches with a power-law variable thickness

Snap-through is an instability phenomenon that occurs in arch and dome-shaped structures, wherein the structure has to move from a stable equilibrium state through an unstable path into another stable equilibrated configuration in a jumping action. In this study, a linear elastic isotropic low arch is considered as a structure with power-law variable thickness. The phenomenon is investigated by considering the equation of the deflection for the variable thickness arch, solving it with an elegant analytical technique, and finding the snap-through critical load from an extreme condition. The effect of power-law exponent and geometry of the arch centerline on critical load is investigated and the constant thickness case and a very rare case of power-law thickness variation found in literature are considered for verification.     

Influence of compliant walls on peristaltic motion with heat/mass transfer and chemical reaction

In this work the fundamental problem of peristalsis with heat and mass transfer is investigated in the presence of a chemical reaction. An incompressible viscous fluid is considered in a channel with compliant walls. Mathematical modeling is based upon the laws of mass, linear momentum, energy and concentration. Analysis is presented not only for long wavelength and low Reynolds number but also for small Grashof number. The solutions are carried out for the stream function, temperature, concentration field and heat transfer coefficient. The results of various interesting parameters are plotted and discussed.