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In the present study we measured calcium-dependent, vesicular glutamate release, and calcium-independent, transport-mediated glutamate release patterns in the vertebrate retina to better understand the sources of elevated glutamate in neural tissue under ischemic conditions. A potassium concentration of 40 mM, which mimics the extracellular potassium concentration in the central nervous system during ischemia, was applied to the bathing medium of a retinal slice prepared from zebrafish. High external potassium evoked release of endogenous glutamate that was measured using a glutamate-specific fluorometric assay applied to the bath. The slice was visualized under 668 nm light using Normarski optics and fluorescent images were captured using a cooled charge-coupled device (CCD) camera. Following the elevation of external potassium to 40 mM several bands of glutamate fluorescence, reflecting the spatial distribution of glutamate release, were observed. A calcium-dependent cloud of glutamate was observed in the inner plexiform layer, that was antagonized by bath-applied nifedipine. A relatively dense glutamate cloud (1-10 microM) was observed over the ganglion cell layer, which was blocked by dihydrokainate, a glutamate transport antagonist. In contrast, nifedipine, an inhibitor of calcium-dependent neurotransmitter release in the retina, failed to block the cloud of released glutamate in the ganglion cell layer. These data suggest that under pathological conditions in the eye where glutamate levels are elevated surrounding retinal ganglion cells, such as observed in some forms of glaucoma, a possible source of the elevated glutamate is through a glutamate transporter operating in a reversed direction. A likely candidate for mediating this reversed transport of glutamate is the retinal Muller cell. 相似文献
13.
A Meddahi J Benoit N Ayoub A Sézeur D Barritault 《Canadian Metallurgical Quarterly》1996,31(3):293-297
OBJECTIVE: To determine whether a short computer interview could be used in place of a full diagnostic interview to obtain psychiatric diagnoses, the authors examined the short interview's sensitivity, specificity, and diagnostic agreement with the full interview. METHODS: Patients recently discharged from a university psychiatric service were interviewed in two back-to-back sessions, one in which a full diagnostic interview was used and the other in which a short computer interview was used. Based on diagnoses derived from both interviews, the sensitivity and specificity of the short interview and kappa values reflecting the diagnostic agreement of the two interviews were calculated. RESULTS: The short interview had high sensitivity and specificity and excellent diagnostic agreement with the full interview for most disorders. It also had a significantly shorter administration time. However, it missed a substantial percentage of cases of generalized anxiety disorder and misclassified as in remission a substantial proportion of patients with active cases of post-traumatic stress disorder. CONCLUSIONS: With few exceptions, the short interview may be substituted for the full interview when missing an active case is not important or when a count of individual symptoms and subtyping of disorders are not needed. Such uses include screening subjects for inclusion in a study and obtaining an overview of a patient's lifetime psychiatric status. 相似文献
14.
An experiment was conducted to examine the role that maximal lifting power has in predicting maximum acceptable weight of lift (MAWL) for a frequency of one lift per 8 h. The secondary aim of the study was to compare the ability of power to predict MAWL to previously used measures of capacity including two measures of isometric strength, five measures of isokinetic strength, and isoinertial capacity on an incremental lifting test. Twenty-five male subjects volunteered to participate in the experiment. The isometric tests involved maximum voluntary contractions for composite lifting strength at vertical heights of 15 and 75 cm. Peak isokinetic strength was measured at velocities of 0.1, 0.2, 0.4, 0.6 and 0.8 m s-1 using a modified CYBEX II isokinetic dynamometer. Isoinertial lifting capacity was measured on the X-factor incremental lifting machine and peak power was measured on the incremental lifting machine by having subjects lift a 25 kg load as quickly as possible. The results indicate that peak isoinertial power is significantly correlated with MAWL, and this correlation was higher than any of the correlations between the other predictor variables and MAWL. The relationships between the isokinetic strength measures and MAWL were stronger than the relationships between the isometric measures and MAWL. Overall, the results suggest that tests used to predict MAWL should be dynamic rather than static. 相似文献
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Kontaxis A Abu-Serriah M Ayoub AF Barbenel JC 《Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine》2004,218(6):381-388
A new method was developed in this study for testing excised sheep mandibles as a cantilever. The method was used to determine the strength and stiffness of sheep hemi-mandibles including a 35 mm defect bridged by regenerated bone. Recombinant human bone morphogenetic protein-7 (rhBMP-7) in a bovine collagen type-I carrier was used for the bone regeneration. Initial tests on ten intact sheep mandibles confirmed that the strength, stiffness and area beneath the load-deformation curves of the right and left hemi-mandibles were not significantly different, confirming the validity of using the contra-lateral hemi-mandible as a control side. Complete bone regeneration occurred in six hemi-mandibles treated with rhBMP, but the quality and mechanical properties of the bone were very variable. The new bone in three samples contained fibrous tissue and was weaker and less stiff than the contra-lateral side (strength, 10-20 per cent; stiffness, 6-15 per cent). The other half had better-quality bone and was significantly stiffer and stronger (p < 0.05), with strength 45-63 per cent and stiffness 35-46 per cent of the contra-lateral side. Hemi-mandibles treated with collagen alone had no regenerated bone bridge suggesting that 35 mm is a critical-size bone defect. 相似文献
17.
Alzoubi F.Y. Alqadi M.K. Al-Khateeb H.M. Ayoub N.Y. 《Applied Superconductivity, IEEE Transactions on》2007,17(3):3814-3818
The interaction force between a small magnet and a superconducting ring in assumptions of the Meissner state was analyzed using the dipole-dipole interaction model. Two configurations were evaluated: the vertical configuration when the magnetic moment lies parallel to the symmetry axis of the ring and the horizontal when it is perpendicular. The levitation force on the magnet increases by decreasing the distance from the center of the ring up to a maximum value and then decreases down to zero when the magnet is exactly at the center of the ring. The levitation force when the magnet is in the horizontal configuration is mostly higher than that when it is in the vertical configuration. Simulation results were compared with experimental results reported in literature. 相似文献
18.
Ayoub Moradi Laurent Metivier Olivier de Viron Stephane Calmant Catherine Mering 《International journal of remote sensing》2013,34(16):6060-6075
Moderate-Resolution Imaging Spectroradiometer (MODIS) optical and infrared data are used to monitor changes in the Caspian Sea coastline. The information extracted from MODIS images is converted into total water volume and mean lake level by combining a digital elevation model (DEM) with remote-sensing data. The elevation estimates were enhanced by reprocessing the MODIS data at the sub-pixel scale. The water volume variations estimated from MODIS data along with DEM are compared to other estimations derived from altimetry data sets, and show fair agreement. 相似文献
19.
A biomechanical evaluation of lifting speed was conducted in the laboratory. The study investigated the effects of lifting speed on several predetermined biomechanical cost functions. The lifting tasks consisted of five lifting speeds labelled as the slowest, slow, normal, fast and fastest, and three weights, 50, 65 and 80% of their maximum acceptable weight of lift. The speed at each level was determined individually by each subject according to their capability. The study found that work-related measures, including the total net muscle work, total absolute net muscle work and work done to the load, decreased significantly as the lifting speed increased (p < 0.05, < 0.001 and < 0.001, respectively). The time integral of sum of squared ratio of joint moment and strength also decreased significantly (p < 0.001). This indicates that lifting at a faster speed tends to reduce the work the body has to do. The peak speed of load occurred at 70% of total lifting time for the slowest lifts, but at 30% of total lifting time for other lifting speeds. Performing lifts at the minimum speeds changed the usual speed coordination technique the subjects used. 相似文献
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