Synthesis and characterization of nanocrystalline NiO-GDC via sodium alginate-mediated ionic sol-gel method

In this study, nanocrystalline nickel oxide gadolinium-doped ceria (NiO-GDC) powder was synthesized in-situ using Na-Alginate as the template via ionic sol-gel technique. The effects of calcination time and temperature on the particle size and the physiochemical properties of nanocrystalline NiO-GDC are presented in this paper. Using this method, gel beads were formed by contacting sodium alginate solution as the gelling template and metal (gadolinium/cerium/Ni) nitrates as the precursor. The obtained nanocrystallites were characterized using Field Emission Scanning Electron Microscopy, powder X-ray diffraction, energy dispersive X-ray spectroscopy, thermo gravimetric analysis, nitrogen adsorption/desorption analysis, and Fourier transform infrared spectroscopy. It was observed that the increasing calcination temperature had affected both the particle size and the surface area of the NiO-GDC, whereas the increasing calcination time had only impacted the size of the particles. The smallest mesoporous nanocrystalline NiO-GDC powder (12.1225 ± 0.005 m²/g surface area), composed of cubic GDC (5.18 nm crystallite size) and cubic NiO (7.99 nm crystallite size) were synthesized at a calcination temperature of 500 °C for 2 h. This study hopes to inspire more researches on the ionic-gelation method for synthesizing other metal nanostructures as well as other reaction parameters.     

Investigation of the antibacterial activity and efflux pump inhibitory effect of co-loaded piperine and gentamicin nanoliposomes in methicillin-resistant Staphylococcus aureus

Objective: Antibiotic resistance has stimulated the research for developing novel strategies that can prevent bacterial growth. Methicillin-resistant Staphylococcus aureus (MRSA), regarded as one of the most serious antibiotic-resistant bacteria which has been conventionally recognized as a nosocomial pathogen.

Materials and methods: Nanoliposomal formulations of piperine and gentamicin were prepared by dehydration-rehydration (DRV) method and characterized for size, zeta potential and encapsulation efficiency. Antibactericidal activities of liposomal and free forms were evaluated against MRSA ATCC 43300 by the determination of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and fractional inhibitory concentration index (FICI). The time-kill studies were carried out to evaluate the potency of antibacterial agents. The effect of piperine on bacterial efflux pumps was also investigated.

Results: MIC values of gentamicin and piperine were 32 and 100?µg/mL, respectively. Synergetic effects were observed by the combination of gentamicin and piperine and FICI was determined to be 0.5. Following incorporation of gentamicin into liposomal gentamicin and liposomal combination, the MIC values were reduced 16- and 32-fold, respectively. MBC values of gentamicin reduced 4 and 8 times following incorporation into gentamicin and combination liposomes, respectively. In comparison with vancomycin, liposomal combination was more effective in bacterial inhibition and killing. Liposomal combination was the most effective preparations in time-kill study. Our findings indicated that liposomal piperine was able to inhibit the efflux pump sufficiently.

Conclusion: The results of this study revealed that liposomal combination is a powerful nano-antibacterial agent to eradicate MRSA infection. This dual-loaded formulation was an effective approach for eradication of MRSA.     

Maximizing Transfection Efficiency of Vertically Aligned Silicon Nanowire Arrays

Vertically aligned silicon nanowire (VA‐SiNW) arrays are emerging as a powerful new tool for gene delivery by means of mechanical transfection. In order to utilize this tool efficiently, uncertainties around the required design parameters need to be removed. Here, a combination of nanosphere lithography and templated metal‐assisted wet chemical etching is used to fabricate VA‐SiNW arrays with a range of diameters, heights, and densities. This fabrication strategy allows identification of critical parameters of surface topography and consequently the design of SiNW arrays that deliver plasmid with high transfection efficiency into a diverse range of human cells whilst maintaining high cell viability. These results illuminate the cell‐materials interactions that mediate VA‐SiNW transfection and have the potential to transform gene therapy and underpin future treatment modalities.     

Resource provisioning based on preempting virtual machines in distributed systems

Resource provisioning is one of the main challenges in large‐scale distributed systems such as federated Grids. Recently, many resource management systems in these environments have started to use the lease abstraction and virtual machines (VMs) for resource provisioning. In the large‐scale distributed systems, resource providers serve requests from external users along with their own local users. The problem arises when there is not sufficient resources for local users, who have higher priority than external ones, and need resources urgently. This problem could be solved by preempting VM‐based leases from external users and allocating them to the local ones. However, preempting VM‐based leases entails side effects in terms of overhead time as well as increasing makespan of external requests. In this paper, we model the overhead of preempting VMs. Then, to reduce the impact of these side effects, we propose and compare several policies that determine the proper set of lease(s) for preemption. We evaluate the proposed policies through simulation as well as real experimentation in the context of InterGrid under different working conditions. Evaluation results demonstrate that the proposed preemption policies serve up to 72% more local requests without increasing the rejection ratio of external requests. Copyright © 2013 John Wiley & Sons, Ltd.     

Effects of conjugated linoleic acid, fish oil and soybean oil on PPARs (α & γ) mRNA expression in broiler chickens and their relation to body fat deposits

An experiment was conducted on broiler chickens to study the effects of different dietary fats (Conjugated linoleic acid (CLA), fish oil, soybean oil, or their mixtures, as well as palm oil, as a more saturated fat), with a as fed dose of 7% for single fat and 3.5 + 3.5% for the mixtures, on Peroxisome Proliferator-Activated Receptors (PPARs) gene expression and its relation with body fat deposits. The CLA used in this experiment was CLA LUTA60 which contained 60% CLA, so 7% and 3.5% dietary inclusions of CLA LUTA60 were equal to 4.2% and 2.1% CLA, respectively. Higher abdominal fat pad was found in broiler chickens fed with a diet containing palm oil compared to chickens in the other experimental groups (P ≤ 0.05). The diets containing CLA resulted in an increased fat deposition in the liver of broiler chickens (P ≤ 0.05). The only exception was related to the birds fed with diets containing palm oil or fish oil + soybean oil, where contents of liver fat were compared to the CLA + fish oil treatment. PPARγ gene in adipose tissue of chickens fed with palm oil diet was up-regulated compared to other treatments (P ≤ 0.001), whereas no significant differences were found in adipose PPARγ gene expression between chickens fed with diets containing CLA, fish oil, soybean oil or the mixture of these fats. On the other hand, the PPARα gene expression in liver tissue was up-regulated in response to the dietary fish oil inclusion and the differences were also significant for both fish oil and CLA + fish oil diets compared to the diets with palm oil, soybean oil or CLA as the only oil source (P ≤ 0.001). In conclusion, the results of present study showed that there was a relationship between the adipose PPARγ gene up-regulation and abdominal fat pad deposition for birds fed with palm oil diet, while no deference was detected in n-3 and n-6 fatty acids, as well as CLA on PPARγ down regulation in comparison to a more saturated fat. When used on its own, fish oil was found to be a more effective fat in up-regulating hepatic PPARα gene expression and this effect was related to a less fat deposition in liver tissue. A negative correlation coefficient (-0.3) between PPARα relative gene expression and liver tissue fat content confirm the anti-lipogenic effect of PPARα, however, the change in these parameters was not completely parallel.     

A new empirical viscosity model for ceramic suspensions

This paper presents a new predictive viscosity model for ceramic suspensions. Starting from Einstein's model (1906), various theoretical, empirical, and phenomenological models have been proposed for different suspension systems. However, there is still a lack of reliable model for ceramic suspensions used in colloidal ceramic shape-forming methods. Here, the rheological properties of ceramic suspensions comprising NiO/YSZ (nickel oxide/yttria stabilized zirconia) as the ceramic powder, and furfuryl alcohol as the suspending media were measured over a range of shear-rates (between 1 and 1000 s⁻¹) and different solid volume fractions from 0 to 0.4010. An empirical equation was then developed for the ceramic suspensions using the mobility parameter (?/(?_m−?)), which links Einstein's model with the more recent relative viscosity models. The proposed model was used to predict the relative viscosity data, showing excellent agreement to the experimental data from this study and with reported data in literature for other ceramic systems. The model was also used to estimate the maximum solid volume fraction for the ceramic suspensions (?_m=0.571), with better accuracy than those estimated by existing models.     

An investigation on the behavior of stiffened coupled shear walls considering axial force effect

In most of tall buildings, the main contribution of lateral loads is carried by coupled shear walls. In some cases, the necessary stiffness to withstand the lateral load may not be afforded due to low depth of connecting beams. In order to increase the capacity of the coupled shear walls, beams with high stiffness are added to the system at particular levels. Hence, stiffened coupled shear walls (SCSW) will be produced. Such walls are under axial load resulting from their weight, and this axial load affects the behavior of walls because of their excessive height. In this paper, a new method considering the effect of axial force for geometrically nonlinear analysis of the SCSW has been presented. A computer program has been developed in matlab , and numerical examples have been solved to demonstrate the reliability of this method. The results of the examples show the agreement between the present method and the other methods given in the literature. The effects of the various positions and rigidities of the stiffening beam on the internal forces and the lateral deflection of the structure considering axial force effect have also been investigated. Copyright © 2012 John Wiley & Sons, Ltd.     

A two-phase genetic K-means algorithm for placement of radioports in cellular networks.

This paper proposes a two-phase hybrid algorithm for optimal placement of radioports. The placement strategy minimizes the dynamic range of the channel, and thus reduces the peak transmit power needed by a wireless handset. The performance of the proposed algorithm is compared with that of an exhaustive search, as well as with that of a simplex algorithm. Simulation results show that the two-phase hybrid algorithm, although suboptimal, outperforms the simplex algorithm with only a modest increase in computation time. It has the added advantage of being quite insensitive to the seed solution.     

Rapid Access Protocols for Discontinuous Transmission in DS-CDMA Systems

In this paper, three reverse link access protocols (AP) enabling discontinuous transmission (DTX) in DS-CDMA personal communication systems are introduced and discussed. The first protocol is synchronous (SDTX-AP), and it uses a so-called synchronous reservation channel to accommodate access requests in a time slotted frame structure. The second uses an asynchronous approach (ADTX-AP) with a spread slotted ALOHA protocol for access requests; access request messages consist of a synchronizing preamble and a user identifier appendix. The third one employs a synchronous structure of overlapping slots, offset in time by a minimum interval (mini-slot) necessary to enable resolution of overlapping access probes sent by different mobile users (MSDTX-AP). Instead of using different spreading codes for different mobile transmitters, all transmitters are assigned the same spreading code to send their access request messages on the access reservation channel. Analysis considers the mean access delay and throughput of the protocols in a multipath fading channel. Numerical results indicate that at high values of offered traffic of access requests mean access delays of ADTX-AP and SDTX-AP are comparable. The mean access delay for the MSDTX-AP depends on the number of minislots or users within each frame, but its throughput is much higher than that of either SDTX-AP or ADTX-AP.