Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA

Reactive intermediate deaminase (Rid) proteins are enzymes conserved in all domains of life. UK114, a mammalian member of RidA subfamily, has been firstly identified as a component of liver perchloric acid-soluble proteins (L-PSP). Although still poorly defined, several functions have been attributed to the mammalian protein UK114/RIDA, including the reactive intermediate deamination activity. The expression of UK114/RIDA has been observed in some tumors, arousing interest in this protein as an evaluable tumor marker. However, other studies reported a negative correlation between UK114/RIDA expression, tumor differentiation degree and cell proliferation. This work addressed the question of UK114/RIDA expression in human non-tumor HEK293 cell lines and in some human tumor cell lines. Here we reported that human RIDA (hRIDA) was expressed in all the analyzed cell line and subjected to lysine (K-)succinylation. In HEK293, hRIDA K-succinylation was negatively correlated to the cell proliferation rate and was under the control of SIRT5. Moreover, K-succinylation clearly altered hRIDA quantification by immunoblotting, explaining, at least in part, some discrepancies about RIDA expression reported in previous studies. We found that hRIDA was able to deaminate reactive enamine-imine intermediates and that K-succinylation drastically reduced deaminase activity. As predicted by in silico analysis, the observed reduction of deaminase activity has been related to the drastic alterations of hRIDA structure inferred by K-succinylation. The role of hRIDA and the importance of its K-succinylation in cell metabolism, especially in cancer biology, have been discussed.     

High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer’s Disease Signatures

The disturbance of protein O-GlcNAcylation is emerging as a possible link between altered brain metabolism and the progression of neurodegeneration. As observed in brains with Alzheimer’s disease (AD), flaws of the cerebral glucose uptake translate into reduced protein O-GlcNAcylation, which promote the formation of pathological hallmarks. A high-fat diet (HFD) is known to foster metabolic dysregulation and insulin resistance in the brain and such effects have been associated with the reduction of cognitive performances. Remarkably, a significant role in HFD-related cognitive decline might be played by aberrant protein O-GlcNAcylation by triggering the development of AD signature and mitochondrial impairment. Our data support the impairment of total protein O-GlcNAcylation profile both in the brain of mice subjected to a 6-week high-fat-diet (HFD) and in our in vitro transposition on SH-SY5Y cells. The reduction of protein O-GlcNAcylation was associated with the development of insulin resistance, induced by overfeeding (i.e., defective insulin signaling and reduced mitochondrial activity), which promoted the dysregulation of the hexosamine biosynthetic pathway (HBP) flux, through the AMPK-driven reduction of GFAT1 activation. Further, we observed that a HFD induced the selective impairment of O-GlcNAcylated-tau and of O-GlcNAcylated-Complex I subunit NDUFB8, thus resulting in tau toxicity and reduced respiratory chain functionality respectively, highlighting the involvement of this posttranslational modification in the neurodegenerative process.     

Sintered stainless steels: Fatigue crack propagation resistance under hydrogen charging conditions

Monophasic and multiphasic (two and three phases) sintered stainless steels were prepared both considering premixes of AISI 316LHC and AISI 434LHC stainless steels powders and using a prealloyed duplex stainless steel 25% Cr, 5% Ni, 2% Mo powder. Their fatigue crack propagation resistance was investigated both in air and under hydrogen charging conditions (0.5 M H₂SO₄ + 0.01 M KSCN aqueous solution; applied potential = −700 mV/SCE), considering three different stress ratios (R = 0.1; 0.5; 0.75). Fatigue crack propagation micromechanisms were investigated by means of fracture surface scanning electron microscope (SEM) analysis.For all the investigated sintered stainless, fatigue crack propagation resistance is influenced by hydrogen charging and an increase of crack growth rates dependent on the steel microstructure is obtained. Experimental results also allow to identify the sintered stainless steel obtained from the prealloyed 25% Cr, 5% Ni, 2% Mo powder as the most resistant to fatigue crack propagation in air and under hydrogen charging conditions.     

试论大型汽车覆盖件模具制造调试的途径

针对汽车大型覆盖件零件的工艺特点，采用先进的模拟仿真技术、测量技术、调试技术、逆向工程技术，探索出了一条汽车大型覆盖件模具制造调试的途径。     

我国型钢生产技术进步20年及展望

按小型材、棒线材、中型材、轨梁和大型材分类 ,对 2 0年来我国在型钢生产领域的主要技术进步进行了总结 ,对其生产现状进行了分析 ,对其发展前景进行了展望     

Effects of Austempering after Hot Deformation on the Mechanical Properties of Hot Rolled Si-Mn TRIP Steel Sheets

Excellent mechanical properties are obtained by austempering after hot deformation without subsequent heat treatment in the present Si-Mn TRIP steel sheets. Isothermal holding time after finishing rolling has affected the mechanical properties of this steel. The results show that the sample exhibits a good combination of ultimate tensile strength and total elongation when it is held at the bainite transformation temperature after hot deformation. The stability of retained austenite increases with an increase of isothermal holding time, and a further increase in the holding duration results in a decrease of it. The tensile strength, total elongation and strength ductility reach the maximum values (774MPa, 33% and 25542MPa% respectively) for this sort of hot rolled Si-Mn TRIP steel using the optimal technology.     

Model of intense pulsed ion beam and simulation study of energy deposited on target

Gaussian type models have been established according to the real detected voltage of magnetically insulated ion diode and intense pulsed ion beam density near the focus of the diode. The energy deposition of Al target irradiated by intense pulsed ion beam has been simulated by Monte Carlo method based on these models. The results of energy depositions of the irradiation of single energy ions and the Gaussian distribution IPIB have been obtained. Especially cases of ion beam irradiating Al target at different angles on one dimension and two dimensions are discussed. The evolution processes of the energy deposition of ions in target have been simulated during a pulse. In addition, incident angles of H⁺ ions and C⁺ ions ranging from 20 to 40° have been researched. We obtained the conclusions that C⁺ ions of the beam affect the physical properties of near surface region, but H⁺ ions affect the deeper layer. The energy of intense pulsed ion beam mainly deposits on target surface, so melting and evaporation begin both from target surface.     

使用人工神经网络对强力旋压有限元模拟进行参数预选

在筒形件强力旋压领域内，介绍了使用人工神经网络的手段，在MARC有限元模拟的前处理中实现参数预选的方法。作为与有限元模拟相结合的旋压智能化设计的一项关键技术，能够达到减少有限元模拟的误差和工作量的目的。     

含Ti微合金钢动态再结晶行为及模型研究

采用Gleeble 3800热力模拟实验机,研究了一种Ti微合金钢在850～1 150℃、0.1和1.0 s-1条件下的变形奥氏体的动态再结晶行为及再结晶后奥氏体晶粒尺寸的演化规律。结果表明:形变激活能与变形条件相关,随着应变速率的增加而增加;Avrami指数m同样与变形条件有关;对实验数据进行回归分析后建立的Ti微合金钢动态再结晶的特征应变、特征应力、动力学以及稳定状态晶粒尺寸的数学模型精度较高。     

小径管焊缝射线照相标准的实践与问题研讨

通过分析小径管焊缝射线检验中存在问题，对X射线检测标准作了进一步探讨。