Synthesis of amphiphilic triblock copolymers for the formation of magnesium fluoride (MgF2) nanoparticles

A series of amphiphilic poly(2‐hydroxyethyl methacrylate)‐b‐polydimethylsiloxane‐b‐poly(2‐hydroxyethyl methacrylate) (pHEMA‐b‐PDMS‐b‐pHEMA) (A‐B‐A) triblock copolymers were synthesized from three different carbinol‐terminated polydimethylsiloxanes with varying molecular weight. A carbinol‐terminated polydimethylsiloxane was modified with 2‐bromoisobutyryl bromide to obtain a macroinitiator. The block copolymers were characterized by NMR, GPC, and dynamic light scattering (DLS). Reverse micelles of a copolymer were formed in mixture of benzene/methanol solution which served as nanoreactors for the synthesis of magnesium fluoride (MgF₂) nanoparticles. The MgF₂ was prepared via chemical precipitation using magnesium chloride and potassium fluoride as reactants. The MgF₂‐triblock copolymer composites were synthesized as a function of MgF₂–weight ratio (0.5, 5, and 10 wt%) in copolymer. The MgF₂ colloids were dissolved in three organic solvents: methanol, isopropanol, and tetrahydrofuran. The polymer nanoparticles were characterized by DLS, transmission electron microscopy, thermogravimetric analysis, and X‐ray diffraction (XRD) analysis. The formation of MgF₂ crystals was observed by XRD. Particle size and particle size distribution showed significant changes in different solvents. The thermal stability of MgF₂ colloids increased as the amount of nanoparticle increased in polymeric matrix. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

Air intrusion and its impact on energy performance of roofing assemblies

Air intrusion through roof assembly is a concern for wind uplift resistance and life-cycle performance of roofs. Airflow control is usually achieved by the installation of an air barrier/retarder in the roofing assembly. Even though the concept of air barrier/retarder and air barrier systems has been around for decades, it is still considered a new notion to the roofing industry. Currently, there are no widely accepted standard specifications or test methods to determine air intrusion through roofs or roof assemblies. An experimental procedure has been under development at the National Research Council of Canada to quantify the air intrusion of roof assemblies. As part of this investigation, five roofing assembly configurations were quantified for air intrusion. Relative performance of the air retarding effect of the five assemblies indicated that assemblies with air barrier/retarder had lower air intrusion rates than without. Measured air intrusion rates are compared with the existing codes of practice and standards. This comparison clearly demonstrates the significance of air intrusion into the roofing assembly and the necessity of a standardized air intrusion test method for the roofing industry. With the measured data, attempts were made to perform thermal load calculations using a simplified procedure, and through two case studies the impact of air intrusion through roofing assemblies on energy performance was estimated.     

Food Protein-polysaccharide Conjugates Obtained via the Maillard Reaction: A Review

The products formed by glycosylation of food proteins with carbohydrates via the Maillard reaction, also known as conjugates, are agents capable of changing and improving techno-functional characteristics of proteins. The Maillard reaction uses the covalent bond between a group of a reducing carbohydrates and an amino group of a protein. This reaction does not require additional chemicals as it occurs naturally under controlled conditions of temperature, time, pH, and moisture. Moreover, there is growing interest in modifying proteins for industrial food applications. This review analyses the current state of art of the Maillard reaction on food protein functionalities. It also discusses the influence of the Maillard reaction on the conditions and formulation of reagents that improve desirable techno-functional characteristics of food protein.     

Deconvolution using signal segmentation

Extraction of peak areas and mass spectral information from chromatography mass spectral data such as obtained in metabolomics measurements requires much effort and the quality is often subjective to the operator that handles the data at hand. In multiple file deconvolution, all samples are processed simultaneously and alignment issues are part of the modeling strategy. However, processing the total data set as a whole is an impossible task and therefore the data processing task requires segmentation. Two intertwined divide and conquer strategies are proposed. The first strategy divides the retention time axis into equal parts and the second strategy divides the total data set into a model and a prediction data set. Dividing the data into smaller segments allows us to conquer the total problem. Post processing of the resulting matrices with peak areas and mass spectra ensures that a matrix with peak areas ready for statistics and a matrix with mass spectral information ready for peak annotation is obtained. The proposed methodology is implemented within a package called TNO-DECO but can easily be implemented in other data pre-processing approaches.     

A rule-based event detection system for real-life underwater domain

Understanding and analyzing fish behaviour is a fundamental task for biologists that study marine ecosystems because the changes in animal behaviour reflect environmental conditions such as pollution and climate change. To support investigators in addressing these complex questions, underwater cameras have been recently used. They can continuously monitor marine life while having almost no influence on the environment under observation, which is not the case with observations made by divers for instance. However, the huge quantity of recorded data make the manual video analysis practically impossible. Thus machine vision approaches are needed to distill the information to be investigated. In this paper, we propose an automatic event detection system able to identify solitary and pairing behaviours of the most common fish species of the Taiwanese coral reef. More specifically, the proposed system employs robust low-level processing modules for fish detection, tracking and recognition that extract the raw data used in the event detection process. Then each fish trajectory is modeled and classified using hidden Markov models. The events of interest are detected by integrating end-user rules, specified through an ad hoc user interface, and the analysis of fish trajectories. The system was tested on 499 events of interest, divided into solitary and pairing events for each fish species. It achieved an average accuracy of 0.105, expressed in terms of normalized detection cost. The obtained results are promising, especially given the difficulties occurring in underwater environments. And moreover, it allows marine biologists to speed up the behaviour analysis process, and to reliably carry on their investigations.     

Relationship between anatomic lesions of the temporal lobe and temporal lobe epilepsy

A structural lesion of the brain is a frequent finding in intractable partial epileptic patients. We analyse anatomo-electro-clinical characteristics of 58 patients in which MR showed a lesion inside the temporal lobe. They are 29 males and 29 females with a mean age at surgery of 23.5 +/- 10.7 years (2.6-45.9). The mean epilepsy duration is of 13.4 +/- 8 years (1.3-35.5), with a mean seizure frequency of 28.7 +/- 43.6 per month, with a great inter-individual variability (from 3 per month to 15 a day). The minimum follow-up is 3.5 years. A video-EEG monitoring was performed in 21 cases, while a stereo-EEG investigation was judged mandatory in 26. On the basis of anatomo-electro-clinical correlations and of the results of presurgical investigations, the epileptogenic area was proved to be temporal in 49 cases, temporal but controlateral to the lesion in 1, and at least bilobar in 8 patients.     

Chlamydia trachomatis antibody detection and diagnosis of reactive arthritis

OBJECTIVE: To investigate whether determining the presence of serum or synovial fluid (SF) IgG and IgA of anti-Chlamydia antibodies with two recent commercially available enzyme-linked immunosorbent assays (ELISA) using synthetic peptides or recombinant antigen could be helpful to detect possible Chlamydia trachomatis (CT)-involved disease in rheumatological patients without evidence of urogenital CT infection. METHODS: The prevalence of such antibodies was determined in samples from patients with well-defined disease, i.e. CT sexually acquired arthritis and from patients with other inflammatory arthropathies unrelated to CT. RESULTS: When considering IgG and/or IgA anti-MOMP or anti-LPS antibodies, a sensitivity of 100% was obtained for serum and SF samples, but with a low specificity. A sensitivity and a specificity equal or close to 80% were observed for the SF IgG anti-MOMP antibodies. CONCLUSION: Clinically, the most appropriate determination was the SF IgG anti-MOMP antibodies. This commercially available ELISA test could be useful for the diagnosis of probable CT reactive arthritis.