Self‐Assembly of Mechanoplasmonic Bacterial Cellulose–Metal Nanoparticle Composites

Nanocomposites of metal nanoparticles (NPs) and bacterial nanocellulose (BC) enable fabrication of soft and biocompatible materials for optical, catalytic, electronic, and biomedical applications. Current BC–NP nanocomposites are typically prepared by in situ synthesis of the NPs or electrostatic adsorption of surface functionalized NPs, which limits possibilities to control and tune NP size, shape, concentration, and surface chemistry and influences the properties and performance of the materials. Here a self‐assembly strategy is described for fabrication of complex and well‐defined BC–NP composites using colloidal gold and silver NPs of different sizes, shapes, and concentrations. The self‐assembly process results in nanocomposites with distinct biophysical and optical properties. In addition to antibacterial materials and materials with excellent senor performance, materials with unique mechanoplasmonic properties are developed. The homogenous incorporation of plasmonic gold NPs in the BC enables extensive modulation of the optical properties by mechanical stimuli. Compression gives rise to near‐field coupling between adsorbed NPs, resulting in tunable spectral variations and enhanced broadband absorption that amplify both nonlinear optical and thermoplasmonic effects and enables novel biosensing strategies.     

Chlorinated-solvent-free gas chromatographic analysis of biomass containing polyhydroxyalkanoates

An extraction and derivatization method was developed for more environmentally friendly routine quantification of polyhydroxyalkanoates (PHAs) in activated sludge biomass by gas chromatography (GC). This method can be further applied to assess relative changes in biomass carbohydrate levels relating to, for example, glycogen or extracellular polysaccharides. Further, co-extracted principal membrane fatty acids are indicative of relative changes in active biomass. The protocol is attractive for routine assessment because it does not require any chlorinated solvents, which are still almost exclusively used today for PHA analysis by GC. Acidic alcoholysis of dried microbial biomass using 3:1 butanol to concentrated (37%) hydrochloric acid at 100 degrees C for 8 h will hydrolyse and derivatize microbial storage products and membrane lipids. Esters of the hydroxyalkanoates, carbohydrates converted to levulinic acid, and long chain microbial fatty acids are reliably extracted into hexane for gas chromatographic analysis and quantification. Calibration can be achieved with benzoic, 2-hydroxyvaleric, or 2-hydroxycaproic acids as the method reference standards.     

Tenants' Dilemma- On Collective Action in Housing

This paper discusses tenant participation in a rational choice perspective, where collective action will neither be initiated, nor maintained, unless found profitable in one way or another by every single individual taking part. This ' n -person prisoners' dilemma' indicates that not the failures but the successful cases of tenant participation call for an explanation. It is claimed that a better understanding of collective action in housing may be developed only if the general and specific conditions of the local housing arena are considered. This includes the following assertions: (1) different tenants may have different motives for taking part in collective action; (2) the dynamics of the relations between tenants must be considered when analysing the conditions of initiating and retaining co-operation; (3) there are different types of collective action in housing, including collective consumption, collective work and collective decision-making; (4) the physical and social conditions of specific housing estates can be more or less favourable to co-operation; (5) tenure forms often define the institutional conditions of collective action in housing.     

Diffractive optical elements designed for highly precise far-field generation in the presence of artifacts typical for pixelated spatial light modulators

Diffractive optical elements (DOEs) realized by spatial light modulators (SLMs) often have features that distinguish them from most conventional, static DOEs: strong coupling between phase and amplitude modulation, a modulation versus steering parameter characteristic that may not be precisely known (and may vary with, e.g., temperature), and deadspace effects and interpixel cross talk. For an optimal function of the DOE, e.g. as a multiple-beam splitter, the DOE design must account for these artifacts. We present an iterative design method in which the optimal setting of each SLM pixel is carefully chosen by considering the SLM artifacts and the design targets. For instance, the deadspace-interpixel effects are modeled by dividing the pixel to be optimized, and its nearest neighbors, into a number of subareas, each with its unique response and far-field contribution. Besides the customary intensity control, the design targets can also include phase control of the optical field in one or more of the beams in the beam splitter. We show how this can be used to cancel a strong unwanted zeroth-order beam, which results from using a slightly incorrect modulation characteristic for the SLM, by purposely sending a beam in the same direction but with the opposite phase. All the designs have been implemented on the 256 x 256 central pixels of a reflective liquid crystal on silicon SLM with a selected input polarization state and a direction of transmission axis of the output polarizer such that for the available different pixel settings a phase modulation of ~2pi rad could be obtained, accompanied by an intensity modulation depth as high as >95%.     

Incoupling waveguide hologram with reduced polarization sensitivity

An incoupling waveguide hologram (IWGH) with significantly reduced polarization sensitivity was designed and fabricated in InP for 1550 nm wavelength. The IWGH couples the light from an optical fiber, irrespectively of the state of polarization, into the InP waveguide and simultaneously focuses it to a desired position in the waveguide. Conventional IWGHs are strongly polarization sensitive with a measured 19 dB difference in the incoupling efficiency between the TE and TM mode. In contrast, although some design parameters turned out to be slightly in error, the fabricated IWGHs designed for reduced polarization sensitivity exhibited a 3.1 dB difference in the incoupling efficiency between the TE and TM modes     

Validation of a rotational coherent anti-Stokes Raman spectroscopy model for carbon dioxide using high-resolution detection in the temperature range 294-1143 K

Experiments were performed in the temperature range of 294-1143 K in pure CO(2) using high-resolution rotational coherent anti-Stokes Raman spectroscopy (CARS), in the dual-broadband approach. Experimental single-shot spectra were recorded with high spectral resolution using a single-mode Nd:YAG laser and a relay imaging lens system on the exit of a 1 m spectrometer. A theoretical rotational CARS model for CO(2) was developed for evaluation of the experimental spectra. The evaluated mean temperatures of the recorded single-shot dual-broadband rotational coherent anti-Stokes Raman spectroscopy (DB-RCARS) spectra using this model showed good agreement with thermocouple temperatures, and the relative standard deviation of evaluated single-shot temperatures was generally 2-3%. Simultaneous thermometry and relative CO(2)/N(2)-concentration measurements were demonstrated in the product gas of premixed laminar CO/air flames at atmospheric pressure. Although the model proved to be accurate for thermometry up to 1143 K, limitations were observed at flame temperatures where temperatures were overestimated and relative CO(2)/N(2) concentrations were underestimated. Potential sources for these discrepancies are discussed.     

Novel Bioassay Demonstrates Attraction of the White Potato Cyst Nematode Globodera Pallida (Stone) to Non-volatile and Volatile Host Plant Cues

Potato cyst nematodes (PCNs) are a major pest of solanaceous crops such as potatoes, tomatoes, and eggplants and have been widely studied over the last 30 years, with the majority of earlier studies focusing on the identification of natural hatching factors. As a novel approach, we focused instead on chemicals involved in nematode orientation towards its host plant. A new dual choice sand bioassay was designed to study nematode responses to potato root exudates (PRE). This bioassay, conducted together with a traditional hatching bioassay, showed that biologically active compounds that induce both hatching and attraction of PCNs can be collected by water extraction of incised potato roots. Furthermore, our results demonstrated that PCN also were attracted by potato root volatiles. Further work is needed to fully understand how PCNs use host plant chemical cues to orientate towards hosts. Nevertheless, the simple attraction assay used in this study provides an important tool for the identification of host-emitted attractants.     

螺旋齿轮与正齿轮的表面致密化

过去几十年,用于汽车的粉末冶金零件不断增长,对零件性能的要求也越来越高.汽车用的变速器齿轮的形状复杂,按照齿轮质量要求(DIN,ISO,AGMA等)几何精度很高,按齿面与齿根的耐久性要求力学性能也很高.对于制造复杂形状零件,粉末冶金法对控制成本很有效.通过增加对齿的选择性致密化,在增加很少费用下就能满足精度与力学性能要求.由压制、烧结、碾压致密化及最后热处理组成的工艺路线,可评估用粉末冶金制造的变速器齿轮的可行性.在研究中所用的螺旋齿轮与正齿轮,对致密化及所得到的齿轮的质量与齿根疲劳都进行了试验.     

Effect of thermal treatment on glucosinolates and antioxidant-related parameters in red cabbage (Brassica oleracea L. ssp. capitata f. rubra)

The effects of various thermal processing treatments (blanching, boiling and steaming) of red cabbage, Brassica oleracea L. ssp. capitata f. rubra cv. ‘Autoro’, were assessed for the levels of glucosinolates (GLS), total phenols (TP), total monomeric anthocyanins (TMA), l-ascorbic acid (L-AA) and soluble sugars, as well as for the antioxidant potential by the ferric reducing ability power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Individual native GLS were determined by ion-pair HPLC-MS/DAD. There were significant (p < 0.05) losses in blanched red cabbage: TP, 43%, TMA 59%, FRAP 42%, ORAC 51%, L-AA 48% and soluble sugars 45%. Boiling gave less extensive reductions: TP 16%, TMA 41%, FRAP 17%, ORAC 19%, L-AA 24% and soluble sugars 19%. Steaming caused no losses for TP, ORAC, FRAP or soluble sugars. However, significant reductions were found for TMA and L-AA, with 29% and 11%, respectively. In general losses were accounted for in the processing waters; however, TMA was not fully recovered, indicating degradation. Total GLS were severely affected by processing, with reductions of 64%, 38% and 19% in blanched, boiled and steamed red cabbage, respectively. Total aliphatic and indole GLS were similarly affected. Lost GLS were partially recovered in the processing water.     

Towards data-driven approaches in manufacturing: an architecture to collect sequences of operations

The technological advancements of recent years have increased the complexity of manufacturing systems, and the ongoing transformation to Industry 4.0 will further aggravate the situation. This is leading to a point where existing systems on the factory floor get outdated, increasing the gap between existing technologies and state-of-the-art systems, making them incompatible. This paper presents an event-based data pipeline architecture, that can be applied to legacy systems as well as new state-of-the-art systems, to collect data from the factory floor. In the presented architecture, actions executed by the resources are converted to event streams, which are then transformed into an abstraction called operations. These operations correspond to the tasks performed in the manufacturing station. A sequence of these operations recount the task performed by the station. We demonstrate the usability of the collected data by using conformance analysis to detect when the manufacturing system has deviated from its defined model. The described architecture is developed in Sequence Planner – a tool for modelling and analysing production systems – and is currently implemented at an automotive company as a pilot project.