Synthesis and release of ATP by soluble mitochondrial F1 in complex with its inhibitor protein during dimethylsulfoxide-water transitions

Soluble mitochondrial F1 and F1 in complex with the natural ATPase inhibitor protein (F1-IP) catalyze the spontaneous synthesis of [gamma-32P]ATP from medium [32P]phosphate and enzyme-bound ADP when incubated in media with dimethylsulfoxide (Me2SO); under these conditions, the synthesized [gamma-32P]ATP is not released into the media, it remains tightly bound to the enzymes [Gómez-Puyou, A., Tuena de Gómez-Puyou, M. & de Meis, L. (1986) Eur. J. Biochem. 159, 133-140]. Some of the characteristics of the synthesized [gamma-32P]ATP were studied in F1 and F1-IP (ATPase activities of 70 and 1-3 micromol x min(-1) x mg(-1), respectively). In Me2SO media, gamma-phosphate of synthesized ATP in F1 or F1-IP exchanges with medium phosphate. From the rates of the exchange reaction, the half-times for hydrolysis of the synthesized ATP in F1 and F1-IP were calculated: 45 min and 58 min for F1 and F1-IP, respectively. The course that synthesized [gamma-32P]ATP follows after dilution of the Me2SO synthetic mixture with aqueous buffer was determined. After dilution, the half-life of synthesized ATP in F1 was less than 1 min. In F1-IP, ATP was also hydrolyzed, but at significantly lower rates. In F1-IP, dilution also produced release of the synthesized [gamma-32P]ATP. This was assayed by the accessibility of [gamma-32P]ATP to hexokinase. About 25% of [gamma-32P]ATP synthesized in F1-IP, but not in F1, was released into the media after dilution with aqueous buffer that contained 20 mM phosphate. Release of tightly bound ATP required the binding energy of phosphate and solvation of F1-IP, however, the particular kinetics of F1-IP were also central for medium ATP synthesis in the absence of electrochemical H+ gradients.     

Optimization of the properties obtained by quenching in martensitic stainless steels X30-40Cr13 and X40-60CrMoV14

An experimental study of the effects produced by different parameters of the heat-quenching cycle permitted the optimization of the properties typical of these martensitic stainless steels: hardness and corrosion resistance. The results obtained demonstrate that the heating temperatures currently used in industrial quenching treatment of these steels should be modified.     

Application of composite resin for single-appointment anterior and posterior diastema closure

Due to aesthetic and periodontal factors, patients often seek treatment to close diastemata. This condition may be eliminated by either direct or indirect techniques. Indirect techniques generally require multiple visits to facilitate the placement of laminate, crown, or bridge restorations, and involve significant financial expense. Direct techniques can be economical and successful, although they present unique challenges in the achievement of satisfactory proximal contacts and contours. This article describes three clinical techniques that may be employed to treat anterior and posterior diastemata in a single appointment.     

Robustly good trellis codes

The relationship between the distance properties of trellis codes and the computational effort and error performance of sequential decoding is studied and optimum distance profile (ODP) and optimum free distance (OFD) trellis codes are constructed for 8-PSK and 16 QAM modulation. A comparison of the performance of both the ODP and the OFD trellis codes reveals that neither class of codes results in the best trade-off between error performance and computational effort when sequential decoding is used. A new algorithm is then proposed to construct robustly good trellis codes for use with sequential decoding. New trellis codes with asymptotic coding gains up to 6.66 dB are obtained using this algorithm, and the new codes achieve nearly the same free distances as the OFD codes and nearly the same distance profiles as the ODP codes     

HIV-1 and HIV-2 antibodies in pregnant women in the City of Maputo, Mozambique. A comparative study between 1982/1983 and 1990

The seroprevalence of HIV-1 and HIV-2 among pregnant women in Maputo, the capital of Mozambique, was compared between the years 1982/83, 1988 and 1990. None of the 432 serum samples collected in 1982/83 was positive for HIV antibodies whereas in 1988 the HIV-1 and HIV-2 seroprevalence was 0.4% (2/500) and 0.6% (3/500), respectively, and in 1990 0.6% (12/2014) and 0.2% (4/2014), respectively. These results indicate that HIV infection has been introduced recently in Maputo and is spreading at a slow rate among women.     

Applications of ultrashort pulse propagation in Bragg gratings forwavelength-division multiplexing and code-division multiple access

We discuss theoretical and experimental studies on the propagation of ultrashort pulses through fiber Bragg gratings. We also consider several applications in optical communications to be found by combining ultrashort pulses and fiber Bragg gratings: a multiwavelength source for wavelength-division-multiplexed systems and a means for implementing optical code-division multiple access     

Optical characterization of low-temperature-grown GaAs forultrafast all-optical switching devices

Low-temperature-grown GaAs (LT-GaAs) is a promising material for all-optical switching devices due to its outstanding optical characteristics. In this paper, we outline a simplified model we have developed to describe the dynamics of the carriers in this material. We also report the results of a series of measurements that we have performed to characterize the optical properties of the material. Specifically, we present the first measurements of the two-photon absorption coefficient and the refractive index changes as a function of the growth and annealing temperatures in LT-GaAs. Finally, we show how our model can be used to optimize the material for applications in all-optical switching     

Spatial soliton-induced guiding by cross-phase modulation

The authors show that spatial solitons are able to induce the stable guiding of a weak probe beam in a homogeneous Kerr-type nonlinear media through cross-phase modulation (XPM). The modes of the induced waveguide are derived from the propagation equation of the probe. Experiments have been performed in Kerr liquid CS₂, with beams of picosecond duration at infrared and visible wavelengths. Stable guiding of a green probe induced by an IR soliton beam has been demonstrated in several different experimental situations. Induced antiguiding and beam splitting are also reported for pump and probe of perpendicular polarizations. All results are in excellent agreement with numerical simulations of the propagation