Human C-peptide. Part I: Radioimmunoassay

Synthetic human C-peptide bearing a Tyrosine group at its amino end is labelled with 125iodine using chloramin T or hydrogen peroxide and lactoperoxidase. The results are compared applying both methods. Antiserum to synthetic human C-peptide (without Tyrosine) which was partially compared to rabbit albumin, is raised in guinea pigs and goats. Goats show to be superior to guinea pigs concerning antibody production. The so-called "hook effect" phenomenon is observed in setting up the standard curves for the radioimmunoassay. Monotonically decreasing standard curves are obtained on dilution of antiserum with a high antibody titer which was produced by repeated immunization in goats. Free C-peptide and C-peptide bound to antiserum are separated with the anxion exchange resin Amberlite. Using this separation technique we excluded unspecific binding of labelled C-peptide to protein fractions in serum of diabetics. The sensitivity of our radioimmunoassay is approx. 0.3 ng C-peptide/ml serum. Intra- and interassay variability are below 10%. Human proinsulin is the only substance found to crossreact with the antiserum.     

TPS6211x电源供应设计的实际考虑

作者:Jeff Falin、Bill Johns·电源管理产品/便携式电源DC/DC应用TPS6211x dc/dc转换器是一种同步降压转换器,其输入电压最高可达17V,输出电压范围为1.2-16V,输出电流高达1.5A。该器件可以高效地将两节锂离子电池、铅酸蓄电池或12V-15V系统电压降至5V、3.3V或者更低。图1为典型应用电路。图1:典型应用本文将提供TPS62111x降压转换器的示例应用并对关键特性进行总结,以供实际设计参考。外部组件的尺寸要求集成FET及内部补偿功能可进一步减少外部组件教。假设不使用集成输出电压与输入电压监控电路,则固定输出电压(5V或3.3V)时仅…     

Estimating the Removal of Anthropogenic Organic Chemicals from Raw Drinking Water by Coagulation Flocculation

A mathematical model was developed to estimate the efficacy of coagulation–flocculation treatment for removing neutral hydrophobic organic chemicals from raw drinking water. The model assumed that the only significant removal mechanism was the destabilization and settling of organic matter containing sorbed anthropogenic organic compounds. The model was validated with standard jar tests using compounds with a range of hydrophobicities (log?Kow = 1.89?to?5.48), including contaminant candidate list chemicals, pesticides, pharmaceuticals, and endocrine disrupting chemicals. Final concentrations of test compounds after coagulation and flocculation were in good agreement with model estimations for synthetic waters composed of Aldrich (Milwaukee, WI) humic acid solutions. The final compound concentrations in coagulated natural waters from two drinking water reservoirs were about 80% lower than those estimated with the model. Overestimations of treated water concentrations by the model were attributed to an increase in sorption by natural organic matter when coiled in aluminum hydroxide flocs, compared to sorption to dispersed natural organic matter in untreated water.     

Grid-Controlled Lightpaths for High Performance Grid Applications

Grid applications call for high performance networking support. One attractive solution is to deploy Grids over optical networks. However, resource management in optical domains is traditionally very rigid and cannot successfully meet the requirements of Grid applications, such as flexible provisioning and configuration. In this paper, we present a customizable resource management solution for optical networks where users can create lightpaths on demand and manage their own network resources. Thanks to a Grid-centric system architecture, lightpath resources can be shared among users and easily integrated with data and computation Grids.     

Towards Efficient Execution of MPI Applications on the Grid: Porting and Optimization Issues

The message passing interface (MPI) is a standard used by many parallel scientific applications. It offers the advantage of a smoother migration path for porting applications from high performance computing systems to the Grid. In this paper Grid-enabled tools and libraries for developing MPI applications are presented. The first is MARMOT, a tool that checks the adherence of an application to the MPI standard. The second is PACX-MPI, an implementation of the MPI standard optimized for Grid environments. Besides the efficient development of the program, an optimal execution is of paramount importance for most scientific applications. We therefore discuss not only performance on the level of the MPI library, but also several application specific optimizations, e.g., for a sparse, parallel equation solver and an RNA folding code, like latency hiding, prefetching, caching and topology-aware algorithms.     

新式笔记本电脑

有谁相信Intel和MIcrosoft所说的一切吗7我也不相信。所以我对过去两周PC Magazine实验室的结果感到吃惊：配备新型1.6GHz Intel Pentium M CPU的centnno系统堪与配备3.06GHz台式机芯片的笔记本媲美，Centrino系统的电池寿命几乎是近期的高端笔记本的两倍。Centrino笔记本几乎具备我们所希望的以及Intel所声称的一切优点。     

更好的浏览器

就性能而非市场份额来说,Opera一直是MicrosoftInternet Explorer的强有力的替代产品在过去两周,我一直在使用Opera 7.1作为我的主要浏览器。我想说它给我的印象深刻。就性能而非市场份额来说,Opera(www.opera.com) 一直是Microsoft InternetExplorer的强有力的替代产品。我感觉Opera比IE要快,并且它还有一个很棒的下载管理器,在一个单独的屏幕上保留着下载文件。再加上一个弹出式阻截器和带有标签的界面,可以将你打开的页面限定在Opera主窗口内。多元搜索窗口把你的搜索请求发送到Google 和AlltheWeb,然后在并列的窗口显示结果。对O…     

2003年采购指南

现在名牌笔记本电脑也卖到1000美元以下了,你也该想想你的下一台PC是否应该是笔记本     

Using network hacking techniques to disrupt military operational networks

It starts in a small way. An Internet-originated electronic mail message received by various technical ratings and Combat Information Center (CIC) officers on a ship deployed in a very high threat area of the Persian Gulf relays news of a natural disaster involving their families that supposedly occurred during a cookout:     

Personal security

I am sitting on a plane, listening to two executives outline their strategy for a major buyout of a bridge/router vendor. Since I am a geek and understand what they are talking about, it's an interesting set of tactics that they are considering to get the price of the company down to something they want. What's even more interesting is how open they are with what they are talking about and, even more, I know the president of the company which they are planning to acquire. It would be very easy for me to make a phone call when we land and completely trash all their plans.